Abstract
Five rice cultivars viz., ASD 16, White Ponni, Pusa Basmati 1, Pusa Sugandh 4 and Pusa Sugandh 5 belonging to subspecies indica were compared for its ability in callus formation and regeneration. In this experiment, the different parameters viz., the effect of hormones (2,4-D and kinetin), organic supplement (coconut milk O1-CM 100 mll-1, O2-CM 75 mll-1, O3-CM 50 mll-1), explants (seed and immature embryo), media (MS and N6), carbon source (sucrose and maltose) using five genotypes on callus response was studied. The effect of hardening methods was also assessed. Results showed that for enhanced callus induction was with MS medium supplemented with 2.0 mgl-1 2, 4-D + 0.5 mgl-1 kinetin + 30 gl-1 maltose irrespective of explants used. Addition of 100 ml l-1 coconut milk was found have improvement in callus response. The performance of immature embryo was better than seed for callus induction, emrbyogenic callus formation, rhizogenic callus formation and regeneration. MS media provided superiority over N6. Among the genotypes Pusa Basmati 1 rendered outstanding performance in callus behavior. The treatment combination MS + 2.5 mgl-1 BAP + 0.5 mgl-1 NAA + 1.0 mgl-1 KN gave the highest organogenesis response and regeneration of plantlets. Hardening in mist chamber was recognized as the best method to give the highest per cent of regenerated plant lets.
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Claims 11
Claim · model-written summary
MS medium with 2,4-D, kinetin and maltose enhanced callus induction across explants.
- Taxon
- rice
Oryza sativat0 - Treatment
- MS medium supplemented with 2.0 mgl-1 2, 4-D + 0.5 mgl-1 kinetin + 30 gl-1 maltosee0
- Outcome
- callus inductione0
- Direction
- enhancede0
- Qualifier
- irrespective of explants usede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhanced callus induction was with MS medium supplemented with 2.0 mgl-1 2, 4-D + 0.5 mgl-1 kinetin + 30 gl-1 maltose irrespective of explants used”
t0“Rice”
Claim · model-written summary
Adding coconut milk improved callus response.
- Taxon
- riceOryza sativat0
- Treatment
- 100 ml l-1 coconut milke0
- Outcome
- callus responsee0
- Direction
- improvemente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of 100 ml l-1 coconut milk was found have improvement in callus response”
t0“Rice”
Claim · model-written summary
Immature embryos performed better than seeds for callus induction.
- Taxon
- riceOryza sativat0
- Treatment
- immature embryoe0
- Outcome
- callus inductione0
- Direction
- bettere0
- Comparator
- seede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The performance of immature embryo was better than seed for callus induction, emrbyogenic callus formation, rhizogenic callus formation and regeneration”
t0“Rice”
Claim · model-written summary
Immature embryos performed better than seeds for embryogenic callus formation.
- Taxon
- riceOryza sativat0
- Treatment
- immature embryoe0
- Outcome
- emrbyogenic callus formatione0
- Direction
- bettere0
- Comparator
- seede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The performance of immature embryo was better than seed for callus induction, emrbyogenic callus formation, rhizogenic callus formation and regeneration”
t0“Rice”
Claim · model-written summary
Immature embryos performed better than seeds for rhizogenic callus formation.
- Taxon
- riceOryza sativat0
- Treatment
- immature embryoe0
- Outcome
- rhizogenic callus formatione0
- Direction
- bettere0
- Comparator
- seede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The performance of immature embryo was better than seed for callus induction, emrbyogenic callus formation, rhizogenic callus formation and regeneration”
t0“Rice”
Claim · model-written summary
Immature embryos performed better than seeds for regeneration.
- Taxon
- riceOryza sativat0
- Treatment
- immature embryoe0
- Outcome
- regeneratione0
- Direction
- bettere0
- Comparator
- seede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The performance of immature embryo was better than seed for callus induction, emrbyogenic callus formation, rhizogenic callus formation and regeneration”
t0“Rice”
Claim · model-written summary
MS medium was superior to N6 for callus response.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS media provided superiority over N6”
t0“Rice”
Claim · model-written summary
Pusa Basmati 1 showed outstanding callus performance among genotypes.
- Taxon
- riceOryza sativat0
- Material
- Pusa Basmati 1e0
- Outcome
- callus behaviore0
- Direction
- outstandinge0
- Comparator
- genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among the genotypes Pusa Basmati 1 rendered outstanding performance in callus behavior”
t0“Rice”
Claim · model-written summary
The BAP, NAA and KN combination gave the highest organogenesis response.
- Taxon
- riceOryza sativat0
- Treatment
- MS + 2.5 mgl-1 BAP + 0.5 mgl-1 NAA + 1.0 mgl-1 KNe0
- Outcome
- organogenesis responsee0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The treatment combination MS + 2.5 mgl-1 BAP + 0.5 mgl-1 NAA + 1.0 mgl-1 KN gave the highest organogenesis response and regeneration of plantlets”
t0“Rice”
Claim · model-written summary
The BAP, NAA and KN combination gave the highest plantlet regeneration.
- Taxon
- riceOryza sativat0
- Treatment
- MS + 2.5 mgl-1 BAP + 0.5 mgl-1 NAA + 1.0 mgl-1 KNe0
- Outcome
- regeneration of plantletse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The treatment combination MS + 2.5 mgl-1 BAP + 0.5 mgl-1 NAA + 1.0 mgl-1 KN gave the highest organogenesis response and regeneration of plantlets”
t0“Rice”
Claim · model-written summary
Mist-chamber hardening gave the highest percentage of regenerated plantlets.
- Taxon
- riceOryza sativat0
- Treatment
- Hardening in mist chambere0
- Outcome
- per cent of regenerated plant letse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hardening in mist chamber was recognized as the best method to give the highest per cent of regenerated plant lets”
t0“Rice”
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