Paper · 2021 · Microorganisms

Effect of Colonization of Trichoderma harzianum on Growth Development and CBD Content of Hemp (Cannabis sativa L.)

Ioanna Kakabouki; Alexandros Tataridas; Antonios Mavroeidis; et al.

9
claims
17
verbatim quotes
47
citations
3
research areas
✓
Open access · cc-by

Abstract

Trichoderma harzianum, as a natural endophytic biocontrol agent, can ameliorate plant development, nutrient uptake, and resistance to biotic and abiotic stresses. This study aimed to investigate the effect of Trichoderma harzianum inoculation on agronomical and quality characteristics of two monoecious hemp (Cannabis sativa L.) varieties, Fedora 17 and Felina. A greenhouse pot experiment was conducted in a completely randomized design of two treatments of Trichoderma harzianum with a low and high dose of the fungus (T1 and T2). The significance of differences between treatments was estimated by using a Fisher’s test with a significance level p = 0.05. The root density of both varieties was significantly affected by treatments, and higher values were recorded in Fedora 17 (2.32 mm cm−3). The Arbuscular Mycorrhizal Fungi (AMF) colonization of the root system and the soil emission of CO2 were higher after the inoculation of Trichoderma harzianum. The highest values of plant height and dry weight were noticed for T2, especially in variety Felina. Trichoderma harzianum positively influenced characteristics of inflorescences such as their number, fresh weight moisture, and compactness in both varieties, while the dry weight, length, and dry yield of inflorescences were not improved. Finally, the fertigation of Trichoderma harzianum in hemp plants was beneficial by increasing the cannabidiol (CBD) content, especially in T2 treatment (4 × 1012 CFU kg−1).

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 9

Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Hemp root density differed significantly among inoculation treatments and was higher in Fedora 17.

Material
Fedora 17e0
Treatment
treatmentse0
Outcome
root densitye0
Direction
highere0
Value
2.32 mm cm−3e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa subsp. sativa
Cannabis sativa subsp. sativa · TaxonNo machine-readable author provided. Bastique assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The root density of both varieties was significantly affected by treatments, and higher values were recorded in Fedora 17 (2.32 mm cm−3)”

✓ located verbatim in the source · abstract, characters 660–796 · show in text ↑
t0

“Hemp (Cannabis sativa L.)”

✓ located verbatim in the source · title, characters 89–114 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Trichoderma inoculation increased AMF colonization of hemp roots.

Treatment
inoculation of Trichoderma harzianume0
Interactor
Arbuscular Mycorrhizal Fungie0symbiont
Second interactor
Trichoderma harzianumTrichoderma harzianume0biocontrol_agent
Outcome
Arbuscular Mycorrhizal Fungi (AMF) colonization of the root systeme0
Direction
highere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa subsp. sativa
Cannabis sativa subsp. sativa · TaxonNo machine-readable author provided. Bastique assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · Second interactorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The Arbuscular Mycorrhizal Fungi (AMF) colonization of the root system and the soil emission of CO2 were higher after the inoculation of Trichoderma harzianum”

✓ located verbatim in the source · abstract, characters 798–956 · show in text ↑
t0

“Hemp (Cannabis sativa L.)”

✓ located verbatim in the source · title, characters 89–114 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Trichoderma inoculation increased soil CO2 emission.

Treatment
inoculation of Trichoderma harzianume0
Interactor
Trichoderma harzianumTrichoderma harzianume0biocontrol_agent
Outcome
soil emission of CO2e0
Direction
highere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa subsp. sativa
Cannabis sativa subsp. sativa · TaxonNo machine-readable author provided. Bastique assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · InteractorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“the soil emission of CO2 were higher after the inoculation of Trichoderma harzianum”

✓ located verbatim in the source · abstract, characters 873–956 · show in text ↑
t0

“Hemp (Cannabis sativa L.)”

✓ located verbatim in the source · title, characters 89–114 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The high Trichoderma dose gave the highest hemp plant height, especially in Felina.

Material
Felinae0
Treatment
T2e0
Interactor
Trichoderma harzianumTrichoderma harzianumt0biocontrol_agent
Outcome
plant heighte0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa subsp. sativa
Cannabis sativa subsp. sativa · TaxonNo machine-readable author provided. Bastique assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · InteractorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The highest values of plant height and dry weight were noticed for T2, especially in variety Felina”

✓ located verbatim in the source · abstract, characters 958–1057 · show in text ↑
t0

“Trichoderma harzianum on Growth Development and CBD Content of Hemp”

✓ located verbatim in the source · title, characters 26–93 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The high Trichoderma dose gave the highest hemp dry weight, especially in Felina.

Material
Felinae0
Treatment
T2e0
Interactor
Trichoderma harzianumTrichoderma harzianumt0biocontrol_agent
Outcome
dry weighte0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa subsp. sativa
Cannabis sativa subsp. sativa · TaxonNo machine-readable author provided. Bastique assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · InteractorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The highest values of plant height and dry weight were noticed for T2, especially in variety Felina”

✓ located verbatim in the source · abstract, characters 958–1057 · show in text ↑
t0

“Trichoderma harzianum on Growth Development and CBD Content of Hemp”

✓ located verbatim in the source · title, characters 26–93 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Trichoderma improved hemp inflorescence number, fresh weight and moisture.

Organ / stage
inflorescencese0
Treatment
Trichoderma harzianume0
Interactor
Trichoderma harzianumTrichoderma harzianume0biocontrol_agent
Outcome
number, fresh weight moisturee0
Direction
positively influencede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa subsp. sativa
Cannabis sativa subsp. sativa · TaxonNo machine-readable author provided. Bastique assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · InteractorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Trichoderma harzianum positively influenced characteristics of inflorescences such as their number, fresh weight moisture, and compactness in both varieties”

✓ located verbatim in the source · abstract, characters 1059–1215 · show in text ↑
t0

“Hemp (Cannabis sativa L.)”

✓ located verbatim in the source · title, characters 89–114 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Trichoderma improved hemp inflorescence compactness.

Organ / stage
inflorescencese0
Treatment
Trichoderma harzianume0
Interactor
Trichoderma harzianumTrichoderma harzianume0biocontrol_agent
Outcome
compactnesse0
Direction
positively influencede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa subsp. sativa
Cannabis sativa subsp. sativa · TaxonNo machine-readable author provided. Bastique assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · InteractorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Trichoderma harzianum positively influenced characteristics of inflorescences such as their number, fresh weight moisture, and compactness in both varieties”

✓ located verbatim in the source · abstract, characters 1059–1215 · show in text ↑
t0

“Hemp (Cannabis sativa L.)”

✓ located verbatim in the source · title, characters 89–114 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Trichoderma did not improve hemp inflorescence dry weight, length or dry yield.

Treatment
Trichoderma harzianumt0
Interactor
Trichoderma harzianumTrichoderma harzianumt0biocontrol_agent
Outcome
dry weight, length, and dry yield of inflorescencese0
Direction
not improvede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa subsp. sativa
Cannabis sativa subsp. sativa · TaxonNo machine-readable author provided. Bastique assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · InteractorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“the dry weight, length, and dry yield of inflorescences were not improved”

✓ located verbatim in the source · abstract, characters 1223–1296 · show in text ↑
t0

“Trichoderma harzianum on Growth Development and CBD Content of Hemp”

✓ located verbatim in the source · title, characters 26–93 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Trichoderma fertigation increased hemp cannabidiol content, especially at the high dose.

Treatment
fertigation of Trichoderma harzianume0
Interactor
Trichoderma harzianumTrichoderma harzianume0biocontrol_agent
Outcome
cannabidiol (CBD) contente0
Direction
increasinge0
Qualifier
T2 treatment (4 × 1012 CFU kg−1)e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa subsp. sativa
Cannabis sativa subsp. sativa · TaxonNo machine-readable author provided. Bastique assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · InteractorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“the fertigation of Trichoderma harzianum in hemp plants was beneficial by increasing the cannabidiol (CBD) content, especially in T2 treatment (4 × 1012 CFU kg−1)”

✓ located verbatim in the source · abstract, characters 1307–1469 · show in text ↑

Trichoderma harzianum · yield · Cannabis sativaPositive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

Trichoderma harzianum · growth · Cannabis sativaResults agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

inoculation · disease · Cannabis sativaPositive and negative results

7 papers: 2 positive, 5 negative or no effect

1 paper in the same direction as this paper · 5 in the opposite direction

  1. negative
  2. negative
  3. negative

3 papers more in this group

See all 7 papers →

fertilization · molecular: metabolites · Cannabis sativaPositive and negative results

8 papers: 4 positive, 4 negative or no effect

3 papers in the same direction as this paper · 4 in the opposite direction

  1. no effect
  2. no effect
  3. no effect

4 papers more in this group

See all 8 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.