Abstract
The Citrus tristeza virus （CTV） uses 3 silencing suppressor genes, p20, p23 and p25, to resist the attacks from its Citrus hosts. Inactivating these genes is therefore obviously a potential defensive option in addition to the current control strat- egies including aphid management and the use of mild strain cross protection. In this study, we cloned partial DNA frag- ments from the three genes, and used them to construct vectors for expressing hairpin RNAs （hpRNAs）. To facilitate the formation of hpRNAs, the constructs were introduced in a loop structure. Following transformation of sour orange （Citrus aurantium） with these constructs, 8 p20 hpRNA （hp20） and 1 p25 hpRNA （hp25） expressing lines were obtained. The 7 hp20 transgenic lines were further characterized. Their reactions to CTV were tested following inoculation with CT14A and/ or TR-L5＂I4, both of which are severe strains. Results showed that 3 lines （hp20-5, hp20-6 and hp20-8） were completely resistant to TR-L514 under greenhouse conditions for no detectable viral load was found in their leaves by PCR. However, they exhibited only partial suppression of TR-L514 under screen house conditions since the virus was detected in their leaves, though 2 months later compared to non-transgenic controls. Further tests showed that hp20-5 was tolerant also to CT14A under screen house conditions. The growth of hp20-5 was much better than others including the controls that were concurrently challenged with CT14A. These results showed that expressing p20 hpRNAwas sufficient to confer sour orange with CTV resistance/tolerance.
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Claims 6
Claim · model-written summary
Eight p20 hpRNA and one p25 hpRNA expressing lines of sour orange were obtained.
- Taxon
- Citrus aurantium
Citrus ×aurantiumt0 - Outcome
- expressing linese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“8 p20 hpRNA （hp20） and 1 p25 hpRNA （hp25） expressing lines were obtained”
t0“Citrus aurantium”
Claim · model-written summary
Three p20 hpRNA lines were completely resistant to TR-L514 under greenhouse conditions, with no detectable viral load in leaves by PCR.
- Taxon
- Citrus aurantiumCitrus ×aurantiumt0
- Interactor
- Citrus tristeza virusCitrus tristeza virust1pathogen
- Outcome
- completely resistant to TR-L514e2
- Qualifier
- no detectable viral load was found in their leaves by PCRe2
- Setting
- greenhouse conditionse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“were completely resistant to TR-L514 under greenhouse conditions for no detectable viral load was found in their leaves by PCR”
t0“Citrus aurantium”
t1“Citrus tristeza virus”
Claim · model-written summary
Under screen house conditions the p20 hpRNA lines gave only partial suppression of TR-L514, with the virus detected in leaves.
- Taxon
- Citrus aurantiumCitrus ×aurantiumt0
- Interactor
- Citrus tristeza virusCitrus tristeza virust1pathogen
- Outcome
- partial suppression of TR-L514e3
- Qualifier
- the virus was detected in their leavese3
- Setting
- screen house conditionse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“they exhibited only partial suppression of TR-L514 under screen house conditions since the virus was detected in their leaves, though 2 months later compared to non-transgenic controls”
t0“Citrus aurantium”
t1“Citrus tristeza virus”
Claim · model-written summary
Line hp20-5 was tolerant to CT14A under screen house conditions.
- Taxon
- Citrus aurantiumCitrus ×aurantiumt0
- Interactor
- CT14Ae6pathogen
- Outcome
- tolerant also to CT14Ae6
- Setting
- screen house conditionse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Further tests showed that hp20-5 was tolerant also to CT14A under screen house conditions”
t0“Citrus aurantium”
Claim · model-written summary
Line hp20-5 grew much better than others, including the controls challenged with CT14A.
- Taxon
- Citrus aurantiumCitrus ×aurantiumt0
- Treatment
- challenged with CT14Ae4
- Interactor
- CT14Ae4pathogen
- Outcome
- growthe4
- Direction
- much bettere4
- Comparator
- the controlse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The growth of hp20-5 was much better than others including the controls that were concurrently challenged with CT14A”
t0“Citrus aurantium”
Claim · model-written summary
Expressing p20 hpRNA was sufficient to confer sour orange with CTV resistance or tolerance.
- Taxon
- Citrus aurantiumCitrus ×aurantiumt0
- Interactor
- CTVe5pathogen
- Outcome
- CTV resistance/tolerancee5
- Qualifier
- expressing p20 hpRNAe5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“These results showed that expressing p20 hpRNAwas sufficient to confer sour orange with CTV resistance/tolerance”
t0“Citrus aurantium”
The same questions in other papers
infection · growth · Citrus x aurantium (sour orange)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.