Abstract
Histidine triad nucleotide binding protein 1(HINT1) plays an important role in many biological processes especially in cell biology, and they have been found in a wide variety of species. However, the functional attributes of HINT1 homologues in plants have not yet been reported. We have previously isolated the full-length cDNA of an HINT1 homologue from maize (Zea mays L.), designated as Zm-HINT1. Subcellular localization was analysed after particle-mediated transient transformation of onion epidermal cells. The result revealed that Zm-HINT1 was targeted to the nucleus. Based on real-time PCR ana- lysis, the expression level of Zm-HINT1 increased signif- icantly after salicylic acid or jasmonic acid treatments. To further investigate the potential physiological role of Zm- HINT1, a binary vector was constructed for Zm-HINT1 synthesis and transgenic Arabidopsis lines were generated by Agrobacterium-mediated transformation. Transgenic Zm-HINT1 plants showed increased resistance to Fusar- ium graminearum compared to the wild type and control plants. Futhermore, real-time PCR results revealed that the pathogen defense genes pathogenesis-related 4 (PR-4) and a peroxidase were upregulated by Zm-HINT1 overexpres- sion. Thus, our data demonstrated that Zm-HINT1 induced antifungal activity against Fusarium graminearum and it may be useful for crop breeding in improving biotic stress tolerance.
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Claims 6
Claim · model-written summary
Zm-HINT1 localized to the nucleus in onion epidermal cells.
- Taxon
- onion
Allium cepae1 - Organ / stage
- epidermal cellse1
- Gene
- Zm-HINT1e0
- Outcome
- targeted to the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zm-HINT1 was targeted to the nucleus”
e1“onion epidermal cells”
Claim · model-written summary
Salicylic acid treatment significantly increased Zm-HINT1 expression.
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression level of Zm-HINT1 increased signif- icantly after salicylic acid or jasmonic acid treatments”
e1“maize (Zea mays L.)”
Claim · model-written summary
Jasmonic acid treatment significantly increased Zm-HINT1 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression level of Zm-HINT1 increased signif- icantly after salicylic acid or jasmonic acid treatments”
e1“maize (Zea mays L.)”
Claim · model-written summary
Transgenic Arabidopsis overexpressing Zm-HINT1 had greater Fusarium resistance than controls.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Treatment
- Transgenic Zm-HINT1e0
- Interactor
- Fusar- ium graminearume0pathogen
- Gene
- Zm-HINT1e0
- Outcome
- resistance to Fusar- ium graminearume0
- Direction
- increasede0
- Comparator
- wild type and control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“Transgenic Zm-HINT1 plants showed increased resistance to Fusar- ium graminearum compared to the wild type and control plants”
Claim · model-written summary
Zm-HINT1 overexpression upregulated PR-4.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Zm-HINT1 overexpres- sione0
- Gene
- pathogenesis-related 4 (PR-4)e0
- Outcome
- pathogenesis-related 4 (PR-4)e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“pathogen defense genes pathogenesis-related 4 (PR-4) and a peroxidase were upregulated by Zm-HINT1 overexpres- sion”
Claim · model-written summary
Zm-HINT1 overexpression upregulated a peroxidase.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Zm-HINT1 overexpres- sione0
- Gene
- a peroxidasee0
- Outcome
- a peroxidasee0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“pathogen defense genes pathogenesis-related 4 (PR-4) and a peroxidase were upregulated by Zm-HINT1 overexpres- sion”
The same questions in other papers
salicylic acid · molecular: expression · Zea mays (maize)Positive and negative results
8 papers in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
7 papers more in this group
See all 11 papers →jasmonic acid · molecular: expression · Zea mays (maize)Results agree
6 papers in the same direction as this paper
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.