Abstract
Drought is a natural hazard that affects crops by inducing water stress. Water stress, induced by drought accounts for more loss in crop yield than all the other causes combined. With the increasing frequency and intensity of droughts worldwide, it is essential to develop drought-resistant crops to ensure food security. In this paper, we model multiple drought signaling pathways in Arabidopsis using Bayesian networks to identify potential regulators of drought-responsive reporter genes. Genetically intervening at these regulators can help develop drought-resistant crops. We create the Bayesian network model from the biological literature and determine its parameters from publicly available data. We conduct inference on this model using a stochastic simulation technique known as likelihood weighting to determine the best regulators of drought-responsive reporter genes. Our analysis reveals that activating MYC2 or inhibiting ATAF1 are the best single node intervention strategies to regulate the drought-responsive reporter genes. Additionally, we observe simultaneously activating MYC2 and inhibiting ATAF1 is a better strategy. The Bayesian network model indicated that MYC2 and ATAF1 are possible regulators of the drought response. Validation experiments showed that ATAF1 negatively regulated the drought response. Thus intervening at ATAF1 has the potential to create drought-resistant crops.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Simulated MYC2 activation was among the best single-node interventions for drought-responsive reporter genes.
- Taxon
- Arabidopsis
Arabidopsise1 - Treatment
- activating MYC2e0
- Gene
- MYC2e0
- Outcome
- drought-responsive reporter genese0
- Value
- best single node intervention strategiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“activating MYC2 or inhibiting ATAF1 are the best single node intervention strategies to regulate the drought-responsive reporter genes”
e1“drought signaling pathways in Arabidopsis”
Claim · model-written summary
Simulated ATAF1 inhibition was among the best single-node interventions for drought-responsive reporter genes.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- inhibiting ATAF1e0
- Gene
- ATAF1e0
- Outcome
- drought-responsive reporter genese0
- Value
- best single node intervention strategiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“activating MYC2 or inhibiting ATAF1 are the best single node intervention strategies to regulate the drought-responsive reporter genes”
e1“drought signaling pathways in Arabidopsis”
Claim · model-written summary
Combined MYC2 activation and ATAF1 inhibition performed better than single-node interventions.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- simultaneously activating MYC2 and inhibiting ATAF1e0
- Outcome
- drought-responsive reporter genese2
- Direction
- bettere0
- Comparator
- best single node intervention strategiese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“simultaneously activating MYC2 and inhibiting ATAF1 is a better strategy”
e1“drought signaling pathways in Arabidopsis”
e2“best single node intervention strategies to regulate the drought-responsive reporter genes”
Claim · model-written summary
The Bayesian model identified MYC2 and ATAF1 as possible drought response regulators.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- MYC2 and ATAF1e0
- Outcome
- possible regulators of the drought responsee0
- Value
- possible regulatorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Bayesian network model indicated that MYC2 and ATAF1 are possible regulators of the drought response.”
e1“drought signaling pathways in Arabidopsis”
Claim · model-written summary
Validation experiments found ATAF1 negatively regulated drought response.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- ATAF1e0
- Outcome
- drought responsee0
- Direction
- negatively regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Validation experiments showed that ATAF1 negatively regulated the drought response.”
e1“drought signaling pathways in Arabidopsis”