Abstract
Myrosinases are β-thioglucoside glucosidases that are unique to the Brassicales order. These enzymes hydrolyze glucosinolates to produce compounds that have direct antibiotic effects or that function as signaling molecules in the plant immune system, protecting plants from pathogens and insect pests. However, the effects of jasmonic acid (JA), a plant hormone that is crucial for plant disease resistance, on myrosinase activity remain unclear. Here, we systematically studied the effects of JA on myrosinase activity and explored the associated internal transcriptional regulation mechanisms. Exogenous application of JA significantly increased myrosinase activity, while the inhibition of endogenous JA biosynthesis and signaling reduced myrosinase activity. In addition, some myrosinase genes in Arabidopsis (Arabidopsis thaliana) were upregulated by JA. Further genetic and biochemical evidence showed that transcription factor FAMA interacted with a series of JASMONATE ZIM-DOMAIN proteins and affected JA-mediated myrosinase activity. However, among the JA-upregulated myrosinase genes, only THIOGLUCOSIDE GLUCOHYDROLASE 1 (TGG1) was positively regulated by FAMA. Further biochemical analysis showed that FAMA bound to the TGG1 promoter to directly mediate TGG1 expression in conjunction with Mediator complex subunit 8 (MED8). Together, our results provide evidence that JA acts as an important signal upstream of the FAMA/MED8-TGG1 pathway to positively regulate myrosinase activity in Arabidopsis.
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Claims 8
Claim · model-written summary
Exogenous JA increased myrosinase activity.
- Taxon
- Arabidopsis
Arabidopsise1 - Treatment
- Exogenous application of JAe0
- Outcome
- myrosinase activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous application of JA significantly increased myrosinase activity”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Inhibiting endogenous JA biosynthesis and signaling reduced myrosinase activity.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- inhibition of endogenous JA biosynthesis and signalinge0
- Outcome
- myrosinase activitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the inhibition of endogenous JA biosynthesis and signaling reduced myrosinase activity”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
JA upregulated some Arabidopsis myrosinase genes.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- JAe0
- Gene
- myrosinase genese0
- Outcome
- myrosinase genese0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“some myrosinase genes in Arabidopsis (Arabidopsis thaliana) were upregulated by JA”
Claim · model-written summary
FAMA interacted with JASMONATE ZIM-DOMAIN proteins.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- FAMAe0
- Outcome
- interacted with a series of JASMONATE ZIM-DOMAIN proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcription factor FAMA interacted with a series of JASMONATE ZIM-DOMAIN proteins”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
FAMA affected JA-mediated myrosinase activity.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- FAMAe0
- Outcome
- JA-mediated myrosinase activitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FAMA interacted with a series of JASMONATE ZIM-DOMAIN proteins and affected JA-mediated myrosinase activity”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
FAMA positively regulated TGG1 among the JA-upregulated myrosinase genes.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- THIOGLUCOSIDE GLUCOHYDROLASE 1 (TGG1)e0
- Outcome
- THIOGLUCOSIDE GLUCOHYDROLASE 1 (TGG1)e0
- Direction
- positively regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“among the JA-upregulated myrosinase genes, only THIOGLUCOSIDE GLUCOHYDROLASE 1 (TGG1) was positively regulated by FAMA”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
FAMA bound the TGG1 promoter and mediated TGG1 expression with MED8.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- TGG1e0
- Outcome
- TGG1 expressione0
- Qualifier
- FAMA bound to the TGG1 promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FAMA bound to the TGG1 promoter to directly mediate TGG1 expression in conjunction with Mediator complex subunit 8 (MED8)”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
JA acts upstream of the FAMA/MED8-TGG1 pathway to positively regulate myrosinase activity.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- FAMA/MED8-TGG1e0
- Outcome
- myrosinase activitye0
- Direction
- positively regulatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JA acts as an important signal upstream of the FAMA/MED8-TGG1 pathway to positively regulate myrosinase activity in Arabidopsis”
The same questions in other papers
jasmonic acid · molecular: expression · ArabidopsisPositive and negative results
20 papers in the same direction as this paper · 13 in the opposite direction
- positive
- negative
- no effect
30 papers more in this group
See all 34 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.