Abstract
Long non-coding RNAs (lncRNAs) play important roles in response to biotic and abiotic stress through acting as competing endogenous RNAs (ceRNAs) to decoy mature miRNAs. However, whether this mechanism is involved in cotton salt stress response remains unknown. We report the characterization of an endogenous lncRNA, lncRNA354, whose expression was reduced in salt-treated cotton and was localized at the nucleus and cytoplasm. Using endogenous target mimic (eTM) analysis, we predicted that lncRNA354 had a potential binding site for miR160b. Transient expression in tobacco demonstrated that lncRNA354 was a miR160b eTM and attenuated miR160b suppression of its target genes, including auxin response factors (ARFs). Silencing or overexpressing lncRNA354 affected the expression of miR160b and target ARFs. Silencing lncRNA354 and targets GhARF17/18 resulted in taller cotton plants and enhanced the resistant to salt stress. Overexpression of lncRNA354 and targets GhARF17/18 in Arabidopsis led to dwarf plants, decreased root dry weight and reduced salt tolerance. Our results show that the lncRNA354-miR160b effect on GhARF17/18 expression may modulate auxin signalling and thus affect growth. These results also shed new light on a mechanism of lncRNA-associated responses to salt stress.
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Claims 9
Claim · model-written summary
lncRNA354 expression was reduced in salt-treated cotton.
- Taxon
- cotton
Gossypium hirsutume0 - Treatment
- salt-treatede0
- Gene
- lncRNA354e0
- Outcome
- expressione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We report the characterization of an endogenous lncRNA, lncRNA354, whose expression was reduced in salt-treated cotton and was localized at the nucleus and cytoplasm”
Claim · model-written summary
lncRNA354 acts as a miR160b eTM and attenuates miR160b suppression of its target ARF genes.
Verbatim
t0“upland cotton”
e1“Transient expression in tobacco demonstrated that lncRNA354 was a miR160b eTM and attenuated miR160b suppression of its target genes, including auxin response factors (ARFs)”
Claim · model-written summary
Silencing or overexpressing lncRNA354 affected the expression of miR160b and target ARFs in cotton.
- Taxon
- upland cottont0
- Treatment
- Silencing or overexpressing lncRNA354e2
- Gene
- lncRNA354e2
- Outcome
- expression of miR160b and target ARFse2
Not reportedComparatorDirection
Verbatim
t0“upland cotton”
e2“Silencing or overexpressing lncRNA354 affected the expression of miR160b and target ARFs”
Claim · model-written summary
Silencing lncRNA354 and GhARF17/18 resulted in taller cotton plants.
- Taxon
- cottonGossypium hirsutume3
- Treatment
- Silencing lncRNA354 and targets GhARF17/18e3
- Gene
- GhARF17/18e3
- Outcome
- cotton plantse3
- Direction
- tallere3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Silencing lncRNA354 and targets GhARF17/18 resulted in taller cotton plants and enhanced the resistant to salt stress”
Claim · model-written summary
Silencing lncRNA354 and GhARF17/18 enhanced resistance to salt stress in cotton.
- Taxon
- cottonGossypium hirsutume3
- Treatment
- Silencing lncRNA354 and targets GhARF17/18e3
- Gene
- GhARF17/18e3
- Outcome
- resistant to salt stresse3
- Direction
- enhancede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Silencing lncRNA354 and targets GhARF17/18 resulted in taller cotton plants and enhanced the resistant to salt stress”
Claim · model-written summary
Overexpression of lncRNA354 and GhARF17/18 in Arabidopsis led to dwarf plants.
- Taxon
- Arabidopsis
Arabidopsise4 - Treatment
- Overexpression of lncRNA354 and targets GhARF17/18e4
- Gene
- GhARF17/18e4
- Outcome
- plantse4
- Direction
- dwarfe4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Overexpression of lncRNA354 and targets GhARF17/18 in Arabidopsis led to dwarf plants, decreased root dry weight and reduced salt tolerance”
Claim · model-written summary
Overexpression of lncRNA354 and GhARF17/18 in Arabidopsis decreased root dry weight.
- Taxon
- ArabidopsisArabidopsise4
- Treatment
- Overexpression of lncRNA354 and targets GhARF17/18e4
- Outcome
- root dry weighte4
- Direction
- decreasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Overexpression of lncRNA354 and targets GhARF17/18 in Arabidopsis led to dwarf plants, decreased root dry weight and reduced salt tolerance”
Claim · model-written summary
Overexpression of lncRNA354 and GhARF17/18 in Arabidopsis reduced salt tolerance.
- Taxon
- ArabidopsisArabidopsise4
- Treatment
- Overexpression of lncRNA354 and targets GhARF17/18e4
- Gene
- GhARF17/18e4
- Outcome
- salt tolerancee4
- Direction
- reducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Overexpression of lncRNA354 and targets GhARF17/18 in Arabidopsis led to dwarf plants, decreased root dry weight and reduced salt tolerance”
Claim · model-written summary
The lncRNA354-miR160b effect on GhARF17/18 may modulate auxin signalling and thus affect growth in cotton.
Verbatim
t0“upland cotton”
e5“Our results show that the lncRNA354-miR160b effect on GhARF17/18 expression may modulate auxin signalling and thus affect growth”
The same questions in other papers
loss of function · disease · Gossypium hirsutum (cotton)Positive and negative results
23 papers in the same direction as this paper · 60 in the opposite direction
- negative
- negative
- negative
80 papers more in this group
See all 84 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.