Abstract
It is widely known that during the reproductive stage (flowering), plants do not root well. Most protocols of shoot regeneration in plants utilize juvenile tissue. Adding these two realities together encouraged us to study the role of florigen in shoot regeneration. Mature tobacco tissue that expresses the endogenous tobacco florigen mRNA regenerates poorly, while juvenile tissue that does not express the florigen regenerates shoots well. Inhibition of Nitric Oxide (NO) synthesis reduced shoot regeneration as well as promoted flowering and increased tobacco florigen level. In contrast, the addition of NO (by way of NO donor) to the tissue increased regeneration, delayed flowering, reduced tobacco florigen mRNA. Ectopic expression of florigen genes in tobacco or tomato decreased regeneration capacity significantly. Overexpression pear PcFT2 gene increased regeneration capacity. During regeneration, florigen mRNA was not changed. We conclude that florigen presence in mature tobacco leaves reduces roots and shoots regeneration and is the possible reason for the age-related decrease in regeneration capacity.
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Claims 11
Claim · model-written summary
Mature tobacco tissue expressing florigen regenerated poorly relative to juvenile tissue.
- Taxon
- tobacco
Nicotiana tabacume0 - Organ / stage
- Mature tobacco tissuee0
- Gene
- tobacco florigen mRNAe0
- Outcome
- regenerates poorlye0
- Direction
- poorlye0
- Comparator
- juvenile tissuee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature tobacco tissue that expresses the endogenous tobacco florigen mRNA regenerates poorly, while juvenile tissue that does not express the florigen regenerates shoots well”
Claim · model-written summary
Inhibiting NO synthesis reduced shoot regeneration.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Inhibition of Nitric Oxide (NO) synthesise0
- Outcome
- shoot regeneratione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition of Nitric Oxide (NO) synthesis reduced shoot regeneration as well as promoted flowering and increased tobacco florigen level”
Claim · model-written summary
Inhibiting NO synthesis promoted flowering.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Inhibition of Nitric Oxide (NO) synthesise0
- Outcome
- floweringe0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition of Nitric Oxide (NO) synthesis reduced shoot regeneration as well as promoted flowering and increased tobacco florigen level”
Claim · model-written summary
Inhibiting NO synthesis increased tobacco florigen level.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Inhibition of Nitric Oxide (NO) synthesise0
- Outcome
- tobacco florigen levele0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition of Nitric Oxide (NO) synthesis reduced shoot regeneration as well as promoted flowering and increased tobacco florigen level”
Claim · model-written summary
NO donor increased regeneration.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- addition of NO (by way of NO donor)e0
- Outcome
- regeneratione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the addition of NO (by way of NO donor) to the tissue increased regeneration, delayed flowering, reduced tobacco florigen mRNA”
e1“Mature tobacco tissue that expresses the endogenous tobacco florigen mRNA”
Claim · model-written summary
NO donor delayed flowering.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- addition of NO (by way of NO donor)e0
- Outcome
- floweringe0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the addition of NO (by way of NO donor) to the tissue increased regeneration, delayed flowering, reduced tobacco florigen mRNA”
Claim · model-written summary
NO donor reduced tobacco florigen mRNA.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- addition of NO (by way of NO donor)e0
- Outcome
- tobacco florigen mRNAe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the addition of NO (by way of NO donor) to the tissue increased regeneration, delayed flowering, reduced tobacco florigen mRNA”
Claim · model-written summary
Ectopic florigen expression in tobacco decreased regeneration capacity.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Ectopic expression of florigen genese0
- Outcome
- regeneration capacitye0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of florigen genes in tobacco or tomato decreased regeneration capacity significantly”
Claim · model-written summary
Ectopic florigen expression in tomato decreased regeneration capacity.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- Ectopic expression of florigen genese0
- Outcome
- regeneration capacitye0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of florigen genes in tobacco or tomato decreased regeneration capacity significantly”
Claim · model-written summary
Overexpressing pear PcFT2 increased regeneration capacity.
- Taxon
- pear
_Poirier_Fleurs_et_feuilles.jpg?width=60)
Pyrus communise0 - Treatment
- Overexpression pear PcFT2 genee0
- Gene
- pear PcFT2 genee0
- Outcome
- regeneration capacitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
e0“Overexpression pear PcFT2 gene increased regeneration capacity”
Claim · model-written summary
Florigen mRNA was unchanged during regeneration.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- florigen mRNAe0
- Direction
- not changede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During regeneration, florigen mRNA was not changed”
e1“Mature tobacco tissue that expresses the endogenous tobacco florigen mRNA”
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See all 11 papers →overexpression · growth · Nicotiana tabacum (tobacco)Positive and negative results
12 papers in the same direction as this paper · 8 in the opposite direction
- positive
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See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.