Abstract
Abstract The STOREKEEPER (STK) family of DNA-binding proteins work as transcription factors and the ectopic expression of two stk-like genes from Arabidopsis thaliana, stk01 (At1g61730) and stk03 (At4g00238), in tobacco increased the number of vegetative internodes and promoted plant and leaf size, stem diameter and sturdiness. The development of these plants started with rosette formation while pronounced shoot elongation and flowering was delayed. Moreover, when the STK01 and STK03 proteins were fused to the Herpes Simplex Virus VP16 transcriptional activation domain and expressed in tobacco the vigorous storekeeper-phenotype did not appear indicating that transgenic STK-like proteins in part worked as repressors of tobacco reproductive development. Furthermore, Yeast Two-Hybrid screenings proved that STK01 and STK03 can form homodimers and heterodimers with further members of the STKlike family. Therefore, we assume that interactions between transgenic Arabidopsis STKs and resident tobacco STKs could have contributed to the observed developmental changes in transgenic tobacco. Our findings open up promising applications for overexpression of stk-like genes in crops that benefit from increased sturdiness and vegetative organ development, such as tobacco in molecular farming approaches, biomass-based energy crops and medicinal plants that produce bioactive compounds in leaves.
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Claims 9
Claim · model-written summary
Ectopic stk01 and stk03 expression increased tobacco vegetative internode number.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- ectopic expression of two stk-like genese0
- Gene
- stk01 (At1g61730) and stk03 (At4g00238)e0
- Outcome
- number of vegetative internodese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ectopic expression of two stk-like genes from Arabidopsis thaliana, stk01 (At1g61730) and stk03 (At4g00238), in tobacco increased the number of vegetative internodes”
Claim · model-written summary
Ectopic stk-like expression promoted tobacco plant and leaf size and stem diameter.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- ectopic expression of two stk-like genese0
- Gene
- stk01 (At1g61730) and stk03 (At4g00238)e0
- Outcome
- plant and leaf size, stem diametere0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ectopic expression of two stk-like genes from Arabidopsis thaliana, stk01 (At1g61730) and stk03 (At4g00238), in tobacco increased the number of vegetative internodes and promoted plant and leaf size, stem diameter and sturdiness.”
Claim · model-written summary
Ectopic stk-like expression promoted tobacco sturdiness.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- storekeeper-transgenict0
- Outcome
- sturdinesse0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promoted plant and leaf size, stem diameter and sturdiness.”
t0“Increased vegetative development and sturdiness of storekeeper-transgenic tobacco”
Claim · model-written summary
The transgenic tobacco plants began development with rosette formation.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- rosettee0
- Outcome
- started with rosette formatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The development of these plants started with rosette formation”
t0“Increased vegetative development and sturdiness of storekeeper-transgenic tobacco”
Claim · model-written summary
Shoot elongation and flowering were delayed in transgenic tobacco.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- shoote0
- Outcome
- shoot elongation and flowering was delayede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pronounced shoot elongation and flowering was delayed.”
t0“Increased vegetative development and sturdiness of storekeeper-transgenic tobacco”
Claim · model-written summary
VP16-fused STK01 and STK03 failed to produce the vigorous phenotype in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- fused to the Herpes Simplex Virus VP16 transcriptional activation domaine0
- Gene
- STK01 and STK03 proteinse0
- Outcome
- vigorous storekeeper-phenotype did not appeare0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when the STK01 and STK03 proteins were fused to the Herpes Simplex Virus VP16 transcriptional activation domain and expressed in tobacco the vigorous storekeeper-phenotype did not appear”
Claim · model-written summary
STK01 and STK03 formed homodimers and heterodimers with other STK family members.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- STK01 and STK03e0
- Outcome
- form homodimers and heterodimerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“STK01 and STK03 can form homodimers and heterodimers with further members of the STKlike family.”
t0“Increased vegetative development and sturdiness of storekeeper-transgenic tobacco”
Claim · model-written summary
The authors inferred that transgenic STK-like proteins partly repress tobacco reproductive development.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- STK-like proteinse0
- Outcome
- repressors of tobacco reproductive developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic STK-like proteins in part worked as repressors of tobacco reproductive development.”
Claim · model-written summary
The authors proposed interactions among Arabidopsis and resident tobacco STKs as a contributor to developmental changes.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- Arabidopsis STKs and resident tobacco STKse0
- Outcome
- developmental changese0
- Qualifier
- could have contributede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“interactions between transgenic Arabidopsis STKs and resident tobacco STKs could have contributed to the observed developmental changes in transgenic tobacco.”
The same questions in other papers
overexpression · growth · Nicotiana tabacum (tobacco)Positive and negative results
7 papers in the same direction as this paper · 13 in the opposite direction
- negative
- negative
- negative
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.