Abstract
The bacterial plant pathogen Pseudomonas syringae uses a type III protein secretion system to inject type III effectors into plant cells. Primary targets of these effectors appear to be effector-triggered immunity (ETI) and pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI). The type III effector HopG1 is a suppressor of ETI that is broadly conserved in bacterial plant pathogens. Here we show that HopG1 from P. syringae pv. tomato DC3000 also suppresses PTI. Interestingly, HopG1 localizes to plant mitochondria, suggesting that its suppression of innate immunity may be linked to a perturbation of mitochondrial function. While HopG1 possesses no obvious mitochondrial signal peptide, its N-terminal two-thirds was sufficient for mitochondrial localization. A HopG1-GFP fusion lacking HopG1's N-terminal 13 amino acids was not localized to the mitochondria reflecting the importance of the N-terminus for targeting. Constitutive expression of HopG1 in Arabidopsis thaliana, Nicotiana tabacum (tobacco) and Lycopersicon esculentum (tomato) dramatically alters plant development resulting in dwarfism, increased branching and infertility. Constitutive expression of HopG1 in planta leads to reduced respiration rates and an increased basal level of reactive oxygen species. These findings suggest that HopG1's target is mitochondrial and that effector/target interaction promotes disease by disrupting mitochondrial functions.
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Claims 10
Claim · model-written summary
HopG1 from Pseudomonas syringae pv. tomato DC3000 suppresses PAMP-triggered immunity in plants.
- Taxon
- plante0
- Treatment
- HopG1e1
- Interactor
- P. syringae pv. tomato DC3000
Pseudomonas syringae pv. tomatoe1pathogen - Gene
- HopG1e1
- Outcome
- PTIe1
- Direction
- suppressese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inject type III effectors into plant cells”
e1“Here we show that HopG1 from P. syringae pv. tomato DC3000 also suppresses PTI”
Claim · model-written summary
HopG1 localizes to plant mitochondria.
Verbatim
e0“inject type III effectors into plant cells”
e2“Interestingly, HopG1 localizes to plant mitochondria, suggesting that its suppression of innate immunity may be linked to a perturbation of mitochondrial function”
Claim · model-written summary
The N-terminal two-thirds of HopG1 is sufficient for mitochondrial localization.
Verbatim
e0“inject type III effectors into plant cells”
e3“While HopG1 possesses no obvious mitochondrial signal peptide, its N-terminal two-thirds was sufficient for mitochondrial localization”
Claim · model-written summary
A HopG1-GFP fusion lacking the N-terminal 13 amino acids was not localized to the mitochondria.
Verbatim
e0“inject type III effectors into plant cells”
e4“A HopG1-GFP fusion lacking HopG1's N-terminal 13 amino acids was not localized to the mitochondria”
Claim · model-written summary
Constitutive expression of HopG1 in Arabidopsis thaliana dramatically altered plant development, causing dwarfism, increased branching and infertility.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae5 - Treatment
- Constitutive expression of HopG1e5
- Gene
- HopG1e5
- Outcome
- plant developmente5
- Qualifier
- dwarfism, increased branching and infertilitye5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Constitutive expression of HopG1 in Arabidopsis thaliana, Nicotiana tabacum (tobacco) and Lycopersicon esculentum (tomato) dramatically alters plant development resulting in dwarfism, increased branching and infertility”
Claim · model-written summary
Constitutive expression of HopG1 in Nicotiana tabacum dramatically altered plant development, causing dwarfism, increased branching and infertility.
- Taxon
- Nicotiana tabacum
Nicotiana tabacume5 - Treatment
- Constitutive expression of HopG1e5
- Gene
- HopG1e5
- Outcome
- plant developmente5
- Qualifier
- dwarfism, increased branching and infertilitye5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Constitutive expression of HopG1 in Arabidopsis thaliana, Nicotiana tabacum (tobacco) and Lycopersicon esculentum (tomato) dramatically alters plant development resulting in dwarfism, increased branching and infertility”
Claim · model-written summary
Constitutive expression of HopG1 in Lycopersicon esculentum dramatically altered plant development, causing dwarfism, increased branching and infertility.
- Taxon
- Lycopersicon esculentum
Solanum lycopersicume5 - Treatment
- Constitutive expression of HopG1e5
- Gene
- HopG1e5
- Outcome
- plant developmente5
- Qualifier
- dwarfism, increased branching and infertilitye5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Constitutive expression of HopG1 in Arabidopsis thaliana, Nicotiana tabacum (tobacco) and Lycopersicon esculentum (tomato) dramatically alters plant development resulting in dwarfism, increased branching and infertility”
Claim · model-written summary
Constitutive expression of HopG1 in planta reduced respiration rates.
- Taxon
- plante0
- Treatment
- Constitutive expression of HopG1e6
- Gene
- HopG1e6
- Outcome
- respiration ratese6
- Direction
- reducede6
Not reportedComparator
Verbatim
e0“inject type III effectors into plant cells”
e6“Constitutive expression of HopG1 in planta leads to reduced respiration rates and an increased basal level of reactive oxygen species”
Claim · model-written summary
Constitutive expression of HopG1 in planta increased the basal level of reactive oxygen species.
- Taxon
- plante0
- Treatment
- Constitutive expression of HopG1e6
- Gene
- HopG1e6
- Outcome
- basal level of reactive oxygen speciese6
- Direction
- increasede6
Not reportedComparator
Verbatim
e0“inject type III effectors into plant cells”
e6“Constitutive expression of HopG1 in planta leads to reduced respiration rates and an increased basal level of reactive oxygen species”
Claim · model-written summary
The authors interpret that the effector-target interaction promotes disease by disrupting mitochondrial functions.
Verbatim
e0“inject type III effectors into plant cells”
e7“effector/target interaction promotes disease by disrupting mitochondrial functions”
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See all 30 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.