Abstract
Ectopic expression of the homeobox gene, NTH15 (Nicotiana tabacum homeobox 15) in transgenic tobacco leads to abnormal leaf and flower morphology, accompanied by a decrease in the content of the active gibberellin, GA1. Quantitative analysis of intermediates in the GA biosynthetic pathway revealed that the step from GA19 to GA20 was blocked in transgenic tobacco plants overexpressing NTH15. To investigate the relationship between the expression of NTH15 and genes involved in GA biosynthesis, we isolated three cDNA clones from tobacco encoding two types of GA 20-oxidase and a 3 beta-hydroxylase. RNA gel blot analysis revealed that the expression of one gene (Ntc12, encoding GA 20-oxidase), which in wild-type tobacco plants was abundantly expressed in leaves, was strongly suppressed in the transformants. The expression level of Ntc12 decreased with increasing severity of phenotype of transgenic tobacco leaves. The abnormal leaf morphology was largely overcome by treatment with GA20 or GA1 but not by GA19. These data strongly suggest that overexpression of NTH15 inhibits the expression of Ntc12, resulting in reduced levels of active GA and abnormal leaf morphology in transgenic tobacco plants. In situ hybridization in wild-type tobacco revealed that expression of Ntc12 occurred mainly in the rib meristem, cells surrounding the procambium and in leaf primordia. Expression was not seen in the tunica, corpus and procambium, tissues in which NTH15 was predominantly expressed. The contrasting expression patterns of these genes may reflect their antagonistic functions in the formation of lateral organs from the shoot apical meristem.
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Claims 15
Claim · model-written summary
Ectopic NTH15 expression causes abnormal tobacco leaf and flower morphology.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- Ectopic expression of the homeobox gene, NTH15e0
- Gene
- NTH15e0
- Outcome
- abnormal leaf and flower morphologye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of the homeobox gene, NTH15 (Nicotiana tabacum homeobox 15) in transgenic tobacco leads to abnormal leaf and flower morphology”
Claim · model-written summary
Ectopic NTH15 expression decreases active gibberellin GA1 content in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Ectopic expression of the homeobox gene, NTH15e0
- Gene
- NTH15e0
- Outcome
- content of the active gibberellin, GA1e0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of the homeobox gene, NTH15 (Nicotiana tabacum homeobox 15) in transgenic tobacco leads to abnormal leaf and flower morphology, accompanied by a decrease in the content of the active gibberellin, GA1”
Claim · model-written summary
The GA19-to-GA20 biosynthetic step was blocked in tobacco overexpressing NTH15.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- overexpressing NTH15e0
- Gene
- NTH15e0
- Outcome
- step from GA19 to GA20e0
- Direction
- blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the step from GA19 to GA20 was blocked in transgenic tobacco plants overexpressing NTH15”
Claim · model-written summary
Three tobacco cDNA clones encoding two GA 20-oxidase types and a 3 beta-hydroxylase were isolated.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- cDNA clonese0
- Value
- threee0
- Qualifier
- encoding two types of GA 20-oxidase and a 3 beta-hydroxylasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated three cDNA clones from tobacco encoding two types of GA 20-oxidase and a 3 beta-hydroxylase”
Claim · model-written summary
Ntc12 was abundantly expressed in wild-type tobacco leaves.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- wild-typee0
- Organ / stage
- leavese0
- Gene
- Ntc12e0
- Outcome
- abundantly expressede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“one gene (Ntc12, encoding GA 20-oxidase), which in wild-type tobacco plants was abundantly expressed in leaves”
Claim · model-written summary
Ntc12 expression was strongly suppressed in NTH15 tobacco transformants.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- overexpressing NTH15e1
- Gene
- Ntc12e0
- Outcome
- expression of one genee0
- Direction
- suppressede0
- Qualifier
- stronglye0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of one gene (Ntc12, encoding GA 20-oxidase), which in wild-type tobacco plants was abundantly expressed in leaves, was strongly suppressed in the transformants”
e1“transgenic tobacco plants overexpressing NTH15”
Claim · model-written summary
Ntc12 expression decreased as the severity of transgenic tobacco leaf phenotype increased.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- leavese0
- Gene
- Ntc12e0
- Outcome
- expression level of Ntc12e0
- Direction
- decreasede0
- Qualifier
- with increasing severity of phenotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression level of Ntc12 decreased with increasing severity of phenotype of transgenic tobacco leaves”
Claim · model-written summary
GA20 treatment largely overcame abnormal tobacco leaf morphology.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- treatment with GA20e0
- Outcome
- abnormal leaf morphologye0
- Direction
- overcomee0
- Qualifier
- largelye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The abnormal leaf morphology was largely overcome by treatment with GA20 or GA1 but not by GA19”
t0“tobacco”
Claim · model-written summary
GA1 treatment largely overcame abnormal tobacco leaf morphology.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- GA1e0
- Outcome
- abnormal leaf morphologye0
- Direction
- overcomee0
- Qualifier
- largelye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The abnormal leaf morphology was largely overcome by treatment with GA20 or GA1 but not by GA19”
t0“tobacco”
Claim · model-written summary
GA19 did not overcome abnormal tobacco leaf morphology.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- GA19e0
- Outcome
- abnormal leaf morphologye0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The abnormal leaf morphology was largely overcome by treatment with GA20 or GA1 but not by GA19”
t0“tobacco”
Claim · model-written summary
The data suggest that NTH15 overexpression inhibits Ntc12 expression, reducing active GA and causing abnormal tobacco leaf morphology.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- NTH15e0
- Outcome
- abnormal leaf morphologye0
- Qualifier
- inhibits the expression of Ntc12, resulting in reduced levels of active GAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These data strongly suggest that overexpression of NTH15 inhibits the expression of Ntc12, resulting in reduced levels of active GA and abnormal leaf morphology in transgenic tobacco plants”
Claim · model-written summary
Wild-type tobacco Ntc12 expression occurred mainly in rib meristem, cells surrounding procambium and leaf primordia.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- wild-typee0
- Organ / stage
- rib meristem, cells surrounding the procambium and in leaf primordiae0
- Gene
- Ntc12e0
- Outcome
- expression of Ntc12e0
- Qualifier
- mainlye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In situ hybridization in wild-type tobacco revealed that expression of Ntc12 occurred mainly in the rib meristem, cells surrounding the procambium and in leaf primordia”
Claim · model-written summary
Ntc12 expression was absent from tunica, corpus and procambium, where NTH15 was predominantly expressed.
- Taxon
- tobaccoNicotiana tabacume1
- Organ / stage
- tunica, corpus and procambiume0
- Gene
- Ntc12e1
- Outcome
- Expression was not seene0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression was not seen in the tunica, corpus and procambium, tissues in which NTH15 was predominantly expressed”
e1“In situ hybridization in wild-type tobacco revealed that expression of Ntc12”
Claim · model-written summary
NTH15 was predominantly expressed in tobacco tunica, corpus and procambium.
- Taxon
- tobaccoNicotiana tabacume1
- Organ / stage
- tunica, corpus and procambiume0
- Gene
- NTH15e0
- Outcome
- predominantly expressede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression was not seen in the tunica, corpus and procambium, tissues in which NTH15 was predominantly expressed”
e1“In situ hybridization in wild-type tobacco”
Claim · model-written summary
Contrasting gene expression patterns may reflect antagonistic Ntc12 and NTH15 functions in lateral organ formation from the tobacco shoot apical meristem.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- shoot apical meristeme0
- Gene
- Ntc12e1
- Outcome
- formation of lateral organse0
- Qualifier
- antagonistic functionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The contrasting expression patterns of these genes may reflect their antagonistic functions in the formation of lateral organs from the shoot apical meristem”
e1“expression of Ntc12 occurred mainly”
e2“NTH15 was predominantly expressed”
t0“tobacco”
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