Paper · 2021 · PLoS ONE

Response of essential oil hemp (Cannabis sativa L.) growth, biomass, and cannabinoid profiles to varying fertigation rates

Steven L. Anderson; Brian Pearson; Roger Kjelgren; et al.

11
claims
22
verbatim quotes
72
citations
2
research areas
✓
Open access · cc-by

Abstract

Five essential oil hemp (Cannabis sativa L.) cultivars (Cherry Blossom, Cherry Blossom (Tuan), Berry Blossom, Cherry Wine, and Cherry Blossom × Trump) were treated with six fertigation treatments to quantify the effects of synthetic fertilizer rates and irrigation electrical conductivity on plant growth, biomass accumulation, and cannabinoid profiles. Irrigation water was injected with a commercial 20-20-20 fertilizer at rates of 0, 50, 150, 300, 450, and 600 ppm nitrogen equating to 0.33 (control), 0.54, 0.96, 1.59, 2.22, and 2.85 dS m-1, respectively. Plants were grown under artificial lighting (18 hr) to maintain vegetative growth for eight weeks, followed by an eight-week flowering period. High linear relationship between chlorophyll concentrations and SPAD-502 measurements validated the utilization of SPAD meters to rapidly identify nutrient deficiency in essential oil hemp. Cultivars expressed significant variation in plant height and cannabinoid profiles (% dry mass), in concurrence with limited biomass and cannabinoid (g per plant) yield variation. Cherry Blossom was the best performing cultivar and Cherry Wine was the least productive. Variation in plant growth, biomass, and cannabinoid concentrations were affected to a greater extent by fertilizer rates. Optimal fertilizer rates were observed at 50 ppm N, while increased fertilizer rates significantly reduced plant growth, biomass accumulation, and cannabinoid concentrations. Increased fertilizer rates (> 300 ppm N) resulted in compliant THC levels (< 0.3%), although when coupled with biomass reductions resulted in minimal cannabinoid yields. Additionally, CBD concentration demonstrated higher sensitivity to increased fertilizer rates (> 300 ppm N) compared to THC and CBG (> 450 ppm N). The results of this study can serve as a guide when using fertigation methods on essential oil hemp cultivars; although results may differ with cultivar selection, environmental conditions, and management practices.

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Claims 11

Associationobservedoriginal resultmeasured

Claim · model-written summary

Chlorophyll concentrations were highly linearly related to SPAD measurements in hemp.

Taxon
Cannabis sativa L.Cannabis sativat0
Outcome
chlorophyll concentrationse0
Direction
High lineare0
Comparator
SPAD-502 measurementse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“High linear relationship between chlorophyll concentrations and SPAD-502 measurements”

✓ located verbatim in the source · abstract, characters 703–788 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 32–50 · show in text ↑
Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Cultivars differed significantly in plant height and cannabinoid profiles.

Taxon
Cannabis sativa L.Cannabis sativat0
Outcome
plant height and cannabinoid profilese0
Direction
variatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Cultivars expressed significant variation in plant height and cannabinoid profiles (% dry mass)”

✓ located verbatim in the source · abstract, characters 893–988 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 32–50 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Cultivars showed limited variation in biomass and per-plant cannabinoid yield.

Taxon
Cannabis sativa L.Cannabis sativat0
Outcome
biomass and cannabinoid (g per plant) yield variatione0
Direction
limitede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“limited biomass and cannabinoid (g per plant) yield variation”

✓ located verbatim in the source · abstract, characters 1010–1071 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 32–50 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Cherry Blossom performed best among the cultivars.

Taxon
Cannabis sativa L.Cannabis sativat0
Material
Cherry Blossome0
Outcome
best performing cultivare0
Direction
beste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Cherry Blossom was the best performing cultivar”

✓ located verbatim in the source · abstract, characters 1073–1120 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 32–50 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Cherry Wine was least productive among the cultivars.

Taxon
Cannabis sativa L.Cannabis sativat0
Material
Cherry Winee0
Outcome
least productivee0
Direction
leaste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Cherry Wine was the least productive”

✓ located verbatim in the source · abstract, characters 1125–1161 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 32–50 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Fertilizer rates affected hemp growth, biomass and cannabinoid concentrations more than cultivar variation.

Taxon
Cannabis sativa L.Cannabis sativat0
Treatment
fertilizer ratese0
Outcome
plant growth, biomass, and cannabinoid concentrationse0
Direction
greater extente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Variation in plant growth, biomass, and cannabinoid concentrations were affected to a greater extent by fertilizer rates.”

✓ located verbatim in the source · abstract, characters 1163–1284 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 32–50 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The observed optimal fertilizer rate was 50 ppm N.

Taxon
Cannabis sativa L.Cannabis sativat0
Outcome
Optimal fertilizer ratese0
Value
50 ppm Ne0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Optimal fertilizer rates were observed at 50 ppm N”

✓ located verbatim in the source · abstract, characters 1285–1335 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 32–50 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Higher fertilizer rates significantly reduced plant growth, biomass accumulation and cannabinoid concentrations.

Taxon
Cannabis sativa L.Cannabis sativat0
Treatment
increased fertilizer ratese0
Outcome
plant growth, biomass accumulation, and cannabinoid concentrationse0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“increased fertilizer rates significantly reduced plant growth, biomass accumulation, and cannabinoid concentrations.”

✓ located verbatim in the source · abstract, characters 1343–1459 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 32–50 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Fertilizer rates above 300 ppm N produced compliant THC levels below 0.3%.

Taxon
Cannabis sativa L.Cannabis sativat0
Treatment
Increased fertilizer rates (> 300 ppm N)e0
Outcome
THC levelse0
Value
< 0.3%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Increased fertilizer rates (> 300 ppm N) resulted in compliant THC levels (< 0.3%)”

✓ located verbatim in the source · abstract, characters 1460–1542 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 32–50 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

At fertilizer rates above 300 ppm N, biomass loss resulted in minimal cannabinoid yields.

Taxon
Cannabis sativa L.Cannabis sativat0
Outcome
cannabinoid yieldse0
Direction
minimale0
Qualifier
biomass reductionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“when coupled with biomass reductions resulted in minimal cannabinoid yields”

✓ located verbatim in the source · abstract, characters 1553–1628 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 32–50 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

CBD concentration was sensitive to fertilizer rates above 300 ppm N, versus THC and CBG above 450 ppm N.

Taxon
Cannabis sativa L.Cannabis sativat0
Treatment
increased fertilizer rates (> 300 ppm N)e0
Outcome
CBD concentratione0
Direction
higher sensitivitye0
Qualifier
> 450 ppm Ne0
Comparator
THC and CBGe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“CBD concentration demonstrated higher sensitivity to increased fertilizer rates (> 300 ppm N) compared to THC and CBG (> 450 ppm N)”

✓ located verbatim in the source · abstract, characters 1644–1775 · show in text ↑
t0

“Cannabis sativa L.”

✓ located verbatim in the source · title, characters 32–50 · show in text ↑

fertilization · growth · Cannabis sativaResults differ

21 papers: 16 positive, 5 negative or no effect

4 papers in the same direction as this paper · 16 in the opposite direction

  1. positive
  2. positive
  3. no effect

17 papers more in this group

See all 21 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.