Paper · 2023 · PLoS ONE

Floral hemp (Cannabis sativa L.) responses to nitrogen fertilization under field conditions in the high desert

Mona M. Farnisa; Glenn C. Miller; Juan K. Q. Solomon; et al.

14
claims
28
verbatim quotes
13
citations
3
research areas
✓
Open access · cc-by

Abstract

While most studies on floral hemp (Cannabis sativa L.) concur that additions of nitrogen (N) increase plant growth, the performance of floral hemp is heavily influenced by environmental conditions, management and cultivar selection. In regions with a short growing season, the availability of soil N may determine plant developmental rates, final inflorescence biomass and cannabinoid concentrations, but no studies have addressed this for field-grown hemp under high-desert conditions. This field study evaluated the effect of no supplemental N and N fertilization at 90 kg ha-1 on three hemp cultivars (Berry Blossom, Red Bordeaux, and Tahoe Cinco) in Northern Nevada. N increased plant height, canopy cover, stem diameter and shoot biomass, but other physiological parameters were dependent on cultivar. For instance, inflorescence biomass and inflorescence-to-shoot ratio in Red Bordeaux was not affected by N fertilization. Similarly, cannabinoid concentrations were affected by timing of harvest and cultivar but not by N treatment. We evaluated the use of a SPAD meter for ease of determining leaf N deficiency, and correlations with leaf chlorophyll content showed that the SPAD meter was a reliable tool in two cultivars but not in Tahoe Cinco. N treatment increased overall CBD yield, which was driven by increases in inflorescence biomass. Tahoe Cinco was the best CBD yielding cultivar, as it maintained a high inflorescence-to-shoot ratio regardless of N treatment. Our study suggests that even though hemp may have a positive response to soil N management, adjustments based on genotype by environment interaction should be aimed at maximizing cannabinoid yield either by increasing biomass and/or CBD concentrations as long as THC levels are within the permissible <0.3% for U.S. industrial hemp cultivation.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 14

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Nitrogen increased height, canopy cover and stem diameter of floral hemp.

Taxon
floral hemp (Cannabis sativa L.)Cannabis sativat0
Treatment
Ne0
Outcome
plant height, canopy cover, stem diametere0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“N increased plant height, canopy cover, stem diameter and shoot biomass, but other physiological parameters were dependent on cultivar.”

✓ located verbatim in the source · abstract, characters 671–806 · show in text ↑
t0

“floral hemp (Cannabis sativa L.)”

✓ located after whitespace/quote normalisation · title, characters 0–32 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Nitrogen increased floral hemp shoot biomass.

Taxon
floral hemp (Cannabis sativa L.)Cannabis sativat0
Treatment
Ne0
Outcome
shoot biomasse0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“N increased plant height, canopy cover, stem diameter and shoot biomass, but other physiological parameters were dependent on cultivar.”

✓ located verbatim in the source · abstract, characters 671–806 · show in text ↑
t0

“floral hemp (Cannabis sativa L.)”

✓ located after whitespace/quote normalisation · title, characters 0–32 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Other physiological responses to nitrogen depended on cultivar.

Taxon
floral hemp (Cannabis sativa L.)Cannabis sativat0
Treatment
Ne0
Outcome
other physiological parameterse0
Qualifier
dependent on cultivare0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“N increased plant height, canopy cover, stem diameter and shoot biomass, but other physiological parameters were dependent on cultivar.”

✓ located verbatim in the source · abstract, characters 671–806 · show in text ↑
t0

“floral hemp (Cannabis sativa L.)”

✓ located after whitespace/quote normalisation · title, characters 0–32 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Nitrogen fertilization did not affect Red Bordeaux inflorescence biomass or inflorescence-to-shoot ratio.

Taxon
floral hemp (Cannabis sativa L.)Cannabis sativat0
Material
Red Bordeauxe0
Treatment
N fertilizatione0
Outcome
inflorescence biomass and inflorescence-to-shoot ratioe0
Direction
not affectede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“For instance, inflorescence biomass and inflorescence-to-shoot ratio in Red Bordeaux was not affected by N fertilization.”

✓ located verbatim in the source · abstract, characters 807–928 · show in text ↑
t0

“floral hemp (Cannabis sativa L.)”

✓ located after whitespace/quote normalisation · title, characters 0–32 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Cannabinoid concentrations varied with harvest timing.

Taxon
floral hemp (Cannabis sativa L.)Cannabis sativat0
Treatment
timing of harveste0
Outcome
cannabinoid concentrationse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Similarly, cannabinoid concentrations were affected by timing of harvest and cultivar but not by N treatment.”

✓ located verbatim in the source · abstract, characters 929–1038 · show in text ↑
t0

“floral hemp (Cannabis sativa L.)”

✓ located after whitespace/quote normalisation · title, characters 0–32 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Cannabinoid concentrations varied with cultivar.

Taxon
floral hemp (Cannabis sativa L.)Cannabis sativat0
Material
cultivare0
Outcome
cannabinoid concentrationse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Similarly, cannabinoid concentrations were affected by timing of harvest and cultivar but not by N treatment.”

✓ located verbatim in the source · abstract, characters 929–1038 · show in text ↑
t0

“floral hemp (Cannabis sativa L.)”

✓ located after whitespace/quote normalisation · title, characters 0–32 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Nitrogen treatment did not affect cannabinoid concentrations.

Taxon
floral hemp (Cannabis sativa L.)Cannabis sativat0
Treatment
N treatmente0
Outcome
cannabinoid concentrationse0
Direction
note0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Similarly, cannabinoid concentrations were affected by timing of harvest and cultivar but not by N treatment.”

✓ located verbatim in the source · abstract, characters 929–1038 · show in text ↑
t0

“floral hemp (Cannabis sativa L.)”

✓ located after whitespace/quote normalisation · title, characters 0–32 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

A SPAD meter reliably indicated leaf nitrogen deficiency in two cultivars.

Taxon
floral hemp (Cannabis sativa L.)Cannabis sativat0
Outcome
leaf N deficiencye0
Value
two cultivarse0
Qualifier
reliable toole0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“We evaluated the use of a SPAD meter for ease of determining leaf N deficiency, and correlations with leaf chlorophyll content showed that the SPAD meter was a reliable tool in two cultivars but not in Tahoe Cinco.”

✓ located verbatim in the source · abstract, characters 1039–1253 · show in text ↑
t0

“floral hemp (Cannabis sativa L.)”

✓ located after whitespace/quote normalisation · title, characters 0–32 · show in text ↑
Method / resourceobservedoriginal resultnegativemeasured

Claim · model-written summary

The SPAD meter was unreliable for Tahoe Cinco.

Taxon
floral hemp (Cannabis sativa L.)Cannabis sativat0
Material
Tahoe Cincoe0
Outcome
leaf N deficiencye0
Value
two cultivarse0
Qualifier
not in Tahoe Cincoe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“We evaluated the use of a SPAD meter for ease of determining leaf N deficiency, and correlations with leaf chlorophyll content showed that the SPAD meter was a reliable tool in two cultivars but not in Tahoe Cinco.”

✓ located verbatim in the source · abstract, characters 1039–1253 · show in text ↑
t0

“floral hemp (Cannabis sativa L.)”

✓ located after whitespace/quote normalisation · title, characters 0–32 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Nitrogen treatment increased overall CBD yield.

Taxon
floral hemp (Cannabis sativa L.)Cannabis sativat0
Treatment
N treatmente0
Outcome
overall CBD yielde0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“N treatment increased overall CBD yield, which was driven by increases in inflorescence biomass.”

✓ located verbatim in the source · abstract, characters 1254–1350 · show in text ↑
t0

“floral hemp (Cannabis sativa L.)”

✓ located after whitespace/quote normalisation · title, characters 0–32 · show in text ↑
Mechanismauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Higher inflorescence biomass drove the increase in CBD yield.

Taxon
floral hemp (Cannabis sativa L.)Cannabis sativat0
Outcome
overall CBD yielde0
Direction
increasede0
Qualifier
increases in inflorescence biomasse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“N treatment increased overall CBD yield, which was driven by increases in inflorescence biomass.”

✓ located verbatim in the source · abstract, characters 1254–1350 · show in text ↑
t0

“floral hemp (Cannabis sativa L.)”

✓ located after whitespace/quote normalisation · title, characters 0–32 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Tahoe Cinco yielded the most CBD.

Taxon
floral hemp (Cannabis sativa L.)Cannabis sativat0
Material
Tahoe Cincoe0
Outcome
CBD yieldinge0
Direction
beste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Tahoe Cinco was the best CBD yielding cultivar, as it maintained a high inflorescence-to-shoot ratio regardless of N treatment.”

✓ located verbatim in the source · abstract, characters 1351–1478 · show in text ↑
t0

“floral hemp (Cannabis sativa L.)”

✓ located after whitespace/quote normalisation · title, characters 0–32 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Tahoe Cinco maintained a high inflorescence-to-shoot ratio regardless of nitrogen treatment.

Taxon
floral hemp (Cannabis sativa L.)Cannabis sativat0
Material
Tahoe Cincoe0
Outcome
inflorescence-to-shoot ratioe0
Direction
highe0
Qualifier
regardless of N treatmente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Tahoe Cinco was the best CBD yielding cultivar, as it maintained a high inflorescence-to-shoot ratio regardless of N treatment.”

✓ located verbatim in the source · abstract, characters 1351–1478 · show in text ↑
t0

“floral hemp (Cannabis sativa L.)”

✓ located after whitespace/quote normalisation · title, characters 0–32 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Management should account for genotype by environment interaction to maximize cannabinoid yield while keeping THC below the permissible limit.

Taxon
floral hemp (Cannabis sativa L.)Cannabis sativat0
Outcome
maximizing cannabinoid yielde0
Qualifier
THC levels are within the permissible <0.3%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Our study suggests that even though hemp may have a positive response to soil N management, adjustments based on genotype by environment interaction should be aimed at maximizing cannabinoid yield either by increasing biomass and/or CBD concentrations as long as THC levels are within the permissible <0.3% for U.S. industrial hemp cultivation.”

✓ located verbatim in the source · abstract, characters 1479–1823 · show in text ↑
t0

“floral hemp (Cannabis sativa L.)”

✓ located after whitespace/quote normalisation · title, characters 0–32 · show in text ↑

fertilization · growth · Cannabis sativaResults differ

21 papers: 16 positive, 5 negative or no effect

4 papers in the same direction as this paper · 16 in the opposite direction

  1. positive
  2. positive
  3. negative

17 papers more in this group

See all 21 papers →

nitrogen · molecular: metabolites · Cannabis sativaPositive and negative results

5 papers: 3 positive, 2 negative or no effect

1 paper in the same direction as this paper · 3 in the opposite direction

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

nitrogen · growth · Cannabis sativaPositive and negative results

14 papers: 11 positive, 3 negative or no effect

10 papers in the same direction as this paper · 3 in the opposite direction

  1. positive
  2. positive
  3. positive

10 papers more in this group

See all 14 papers →

nitrogen · yield · Cannabis sativaPositive and negative results

16 papers: 15 positive, 1 negative or no effect

14 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. positive
  3. positive

12 papers more in this group

See all 16 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.