Abstract
Pre-harvest sprouting (PHS) reduces yields and grain quality, resulting in seriously economic losses in wheat. It has been showed that PHS is significantly correlated to seed dormancy levels. FUSCA3 (FUS3) gene is considered to be the key regulator of seed dormancy. However, little information is available about the function of FUS3 gene (TaFUS3) in wheat. In this study, three homologous genes were identified in wheat grain, and their functions were investigated by gene silencing. Three full-length DNA (3477, 3534 and 3501 bp) and cDNA (1015, 1012 and 1015 bp) sequences encoding a B3 transcription factor, designated TaFUS3-3A, TaFUS3-3B and TaFUS3-3D, were first isolated from common wheat. The transcription of three TaFUS3 genes in seed development and germination process was detected. TaFUS3-3B and TaFUS3-3D had similar expression profiles, and high levels of gene transcripts were detected in seeds at 25 DAP (days after pollination) and after 24 h of imbibition. However, the transcription of TaFUS3-3A was not detected. Silencing of TaFUS3 in common wheat spikes resulted in increased seed germination and PHS. Compared with wild-type, the TaFUS3-silenced plants showed increased expression of genes related to GA biosynthesis and ABA metabolism, and decreased expression of genes associated with ABA biosynthesis. Moreover, silencing of TaFUS3 in wheat plants led to a decrease in embryo sensitivity to ABA and changed the expression of genes involved in ABA signal transduction. The results of gene silencing indicated that TaFUS3 plays a positive role in wheat seed dormancy and PHS-resistance, which might be associated with ABA, GA level and signal transduction.
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Claims 9
Claim · model-written summary
Three TaFUS3 homologs were isolated in wheat.
- Taxon
- common wheat
Triticum aestivume0 - Outcome
- TaFUS3-3A, TaFUS3-3B and TaFUS3-3De0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“designated TaFUS3-3A, TaFUS3-3B and TaFUS3-3D, were first isolated from common wheat”
e1“Pre-harvest sprouting (PHS) reduces yields and grain quality, resulting in seriously economic losses in wheat. It has been showed that PHS is significantly correlated to seed dormancy levels. FUSCA3 (FUS3) gene is considered to be the key regulator of seed dormancy. However, little information is available about the function of FUS3 gene (TaFUS3) in wheat. In this study, three homologous genes were identified in wheat grain, and their functions were investigated by gene silencing. Three full-length DNA (3477, 3534 and 3501 bp) and cDNA (1015, 1012 and 1015 bp) sequences encoding a B3 transcription factor, designated TaFUS3-3A, TaFUS3-3B and TaFUS3-3D, were first isolated from common wheat. The transcription of three TaFUS3 genes in seed development and germination process was detected. TaFUS3-3B and TaFUS3-3D had similar expression profiles, and high levels of gene transcripts were detected in seeds at 25 DAP (days after pollination) and after 24 h of imbibition. However, the transcription of TaFUS3-3A was not detected. Silencing of TaFUS3 in common wheat spikes resulted in increased seed germination and PHS. Compared with wild-type, the TaFUS3-silenced plants showed increased expression of genes related to GA biosynthesis and ABA metabolism, and decreased expression of genes associated with ABA biosynthesis. Moreover, silencing of TaFUS3 in wheat plants led to a decrease in embryo sensitivity to ABA and changed the expression of genes involved in ABA signal transduction. The results of gene silencing indicated that TaFUS3 plays a positive role in wheat seed dormancy and PHS-resistance, which might be associated with ABA, GA level and signal transduction.”
Claim · model-written summary
TaFUS3-3B and TaFUS3-3D had similar expression profiles and high seed transcript levels at 25 DAP and 24 h imbibition.
- Taxon
- wheatTriticum aestivume1
- Organ / stage
- seedse0
- Gene
- TaFUS3-3B and TaFUS3-3De0
- Outcome
- high levels of gene transcriptse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaFUS3-3B and TaFUS3-3D had similar expression profiles, and high levels of gene transcripts were detected in seeds at 25 DAP (days after pollination) and after 24 h of imbibition”
e1“Pre-harvest sprouting (PHS) reduces yields and grain quality, resulting in seriously economic losses in wheat. It has been showed that PHS is significantly correlated to seed dormancy levels. FUSCA3 (FUS3) gene is considered to be the key regulator of seed dormancy. However, little information is available about the function of FUS3 gene (TaFUS3) in wheat. In this study, three homologous genes were identified in wheat grain, and their functions were investigated by gene silencing. Three full-length DNA (3477, 3534 and 3501 bp) and cDNA (1015, 1012 and 1015 bp) sequences encoding a B3 transcription factor, designated TaFUS3-3A, TaFUS3-3B and TaFUS3-3D, were first isolated from common wheat. The transcription of three TaFUS3 genes in seed development and germination process was detected. TaFUS3-3B and TaFUS3-3D had similar expression profiles, and high levels of gene transcripts were detected in seeds at 25 DAP (days after pollination) and after 24 h of imbibition. However, the transcription of TaFUS3-3A was not detected. Silencing of TaFUS3 in common wheat spikes resulted in increased seed germination and PHS. Compared with wild-type, the TaFUS3-silenced plants showed increased expression of genes related to GA biosynthesis and ABA metabolism, and decreased expression of genes associated with ABA biosynthesis. Moreover, silencing of TaFUS3 in wheat plants led to a decrease in embryo sensitivity to ABA and changed the expression of genes involved in ABA signal transduction. The results of gene silencing indicated that TaFUS3 plays a positive role in wheat seed dormancy and PHS-resistance, which might be associated with ABA, GA level and signal transduction.”
Claim · model-written summary
TaFUS3-3A transcription was not detected.
- Taxon
- wheatTriticum aestivume1
- Gene
- TaFUS3-3Ae0
- Outcome
- transcription of TaFUS3-3A was not detectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcription of TaFUS3-3A was not detected”
e1“Pre-harvest sprouting (PHS) reduces yields and grain quality, resulting in seriously economic losses in wheat. It has been showed that PHS is significantly correlated to seed dormancy levels. FUSCA3 (FUS3) gene is considered to be the key regulator of seed dormancy. However, little information is available about the function of FUS3 gene (TaFUS3) in wheat. In this study, three homologous genes were identified in wheat grain, and their functions were investigated by gene silencing. Three full-length DNA (3477, 3534 and 3501 bp) and cDNA (1015, 1012 and 1015 bp) sequences encoding a B3 transcription factor, designated TaFUS3-3A, TaFUS3-3B and TaFUS3-3D, were first isolated from common wheat. The transcription of three TaFUS3 genes in seed development and germination process was detected. TaFUS3-3B and TaFUS3-3D had similar expression profiles, and high levels of gene transcripts were detected in seeds at 25 DAP (days after pollination) and after 24 h of imbibition. However, the transcription of TaFUS3-3A was not detected. Silencing of TaFUS3 in common wheat spikes resulted in increased seed germination and PHS. Compared with wild-type, the TaFUS3-silenced plants showed increased expression of genes related to GA biosynthesis and ABA metabolism, and decreased expression of genes associated with ABA biosynthesis. Moreover, silencing of TaFUS3 in wheat plants led to a decrease in embryo sensitivity to ABA and changed the expression of genes involved in ABA signal transduction. The results of gene silencing indicated that TaFUS3 plays a positive role in wheat seed dormancy and PHS-resistance, which might be associated with ABA, GA level and signal transduction.”
Claim · model-written summary
Silencing TaFUS3 increased wheat seed germination and pre-harvest sprouting.
- Taxon
- common wheatTriticum aestivume0
- Treatment
- Silencing of TaFUS3e0
- Gene
- TaFUS3e0
- Outcome
- seed germination and PHSe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of TaFUS3 in common wheat spikes resulted in increased seed germination and PHS”
e1“Pre-harvest sprouting (PHS) reduces yields and grain quality, resulting in seriously economic losses in wheat. It has been showed that PHS is significantly correlated to seed dormancy levels. FUSCA3 (FUS3) gene is considered to be the key regulator of seed dormancy. However, little information is available about the function of FUS3 gene (TaFUS3) in wheat. In this study, three homologous genes were identified in wheat grain, and their functions were investigated by gene silencing. Three full-length DNA (3477, 3534 and 3501 bp) and cDNA (1015, 1012 and 1015 bp) sequences encoding a B3 transcription factor, designated TaFUS3-3A, TaFUS3-3B and TaFUS3-3D, were first isolated from common wheat. The transcription of three TaFUS3 genes in seed development and germination process was detected. TaFUS3-3B and TaFUS3-3D had similar expression profiles, and high levels of gene transcripts were detected in seeds at 25 DAP (days after pollination) and after 24 h of imbibition. However, the transcription of TaFUS3-3A was not detected. Silencing of TaFUS3 in common wheat spikes resulted in increased seed germination and PHS. Compared with wild-type, the TaFUS3-silenced plants showed increased expression of genes related to GA biosynthesis and ABA metabolism, and decreased expression of genes associated with ABA biosynthesis. Moreover, silencing of TaFUS3 in wheat plants led to a decrease in embryo sensitivity to ABA and changed the expression of genes involved in ABA signal transduction. The results of gene silencing indicated that TaFUS3 plays a positive role in wheat seed dormancy and PHS-resistance, which might be associated with ABA, GA level and signal transduction.”
Claim · model-written summary
TaFUS3 silencing increased expression of genes for GA biosynthesis and ABA metabolism.
- Taxon
- wheatTriticum aestivume1
- Treatment
- TaFUS3-silencede0
- Outcome
- expression of genes related to GA biosynthesis and ABA metabolisme0
- Direction
- increasede0
- Comparator
- wild-typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaFUS3-silenced plants showed increased expression of genes related to GA biosynthesis and ABA metabolism”
e1“Pre-harvest sprouting (PHS) reduces yields and grain quality, resulting in seriously economic losses in wheat. It has been showed that PHS is significantly correlated to seed dormancy levels. FUSCA3 (FUS3) gene is considered to be the key regulator of seed dormancy. However, little information is available about the function of FUS3 gene (TaFUS3) in wheat. In this study, three homologous genes were identified in wheat grain, and their functions were investigated by gene silencing. Three full-length DNA (3477, 3534 and 3501 bp) and cDNA (1015, 1012 and 1015 bp) sequences encoding a B3 transcription factor, designated TaFUS3-3A, TaFUS3-3B and TaFUS3-3D, were first isolated from common wheat. The transcription of three TaFUS3 genes in seed development and germination process was detected. TaFUS3-3B and TaFUS3-3D had similar expression profiles, and high levels of gene transcripts were detected in seeds at 25 DAP (days after pollination) and after 24 h of imbibition. However, the transcription of TaFUS3-3A was not detected. Silencing of TaFUS3 in common wheat spikes resulted in increased seed germination and PHS. Compared with wild-type, the TaFUS3-silenced plants showed increased expression of genes related to GA biosynthesis and ABA metabolism, and decreased expression of genes associated with ABA biosynthesis. Moreover, silencing of TaFUS3 in wheat plants led to a decrease in embryo sensitivity to ABA and changed the expression of genes involved in ABA signal transduction. The results of gene silencing indicated that TaFUS3 plays a positive role in wheat seed dormancy and PHS-resistance, which might be associated with ABA, GA level and signal transduction.”
Claim · model-written summary
TaFUS3 silencing decreased expression of genes for ABA biosynthesis.
- Taxon
- wheatTriticum aestivume1
- Treatment
- TaFUS3-silencede1
- Outcome
- expression of genes associated with ABA biosynthesise0
- Direction
- decreasede0
- Comparator
- wild-typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“decreased expression of genes associated with ABA biosynthesis”
e1“Pre-harvest sprouting (PHS) reduces yields and grain quality, resulting in seriously economic losses in wheat. It has been showed that PHS is significantly correlated to seed dormancy levels. FUSCA3 (FUS3) gene is considered to be the key regulator of seed dormancy. However, little information is available about the function of FUS3 gene (TaFUS3) in wheat. In this study, three homologous genes were identified in wheat grain, and their functions were investigated by gene silencing. Three full-length DNA (3477, 3534 and 3501 bp) and cDNA (1015, 1012 and 1015 bp) sequences encoding a B3 transcription factor, designated TaFUS3-3A, TaFUS3-3B and TaFUS3-3D, were first isolated from common wheat. The transcription of three TaFUS3 genes in seed development and germination process was detected. TaFUS3-3B and TaFUS3-3D had similar expression profiles, and high levels of gene transcripts were detected in seeds at 25 DAP (days after pollination) and after 24 h of imbibition. However, the transcription of TaFUS3-3A was not detected. Silencing of TaFUS3 in common wheat spikes resulted in increased seed germination and PHS. Compared with wild-type, the TaFUS3-silenced plants showed increased expression of genes related to GA biosynthesis and ABA metabolism, and decreased expression of genes associated with ABA biosynthesis. Moreover, silencing of TaFUS3 in wheat plants led to a decrease in embryo sensitivity to ABA and changed the expression of genes involved in ABA signal transduction. The results of gene silencing indicated that TaFUS3 plays a positive role in wheat seed dormancy and PHS-resistance, which might be associated with ABA, GA level and signal transduction.”
Claim · model-written summary
TaFUS3 silencing decreased embryo sensitivity to ABA.
- Taxon
- wheatTriticum aestivume0
- Treatment
- silencing of TaFUS3e0
- Outcome
- embryo sensitivity to ABAe0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of TaFUS3 in wheat plants led to a decrease in embryo sensitivity to ABA”
e1“Pre-harvest sprouting (PHS) reduces yields and grain quality, resulting in seriously economic losses in wheat. It has been showed that PHS is significantly correlated to seed dormancy levels. FUSCA3 (FUS3) gene is considered to be the key regulator of seed dormancy. However, little information is available about the function of FUS3 gene (TaFUS3) in wheat. In this study, three homologous genes were identified in wheat grain, and their functions were investigated by gene silencing. Three full-length DNA (3477, 3534 and 3501 bp) and cDNA (1015, 1012 and 1015 bp) sequences encoding a B3 transcription factor, designated TaFUS3-3A, TaFUS3-3B and TaFUS3-3D, were first isolated from common wheat. The transcription of three TaFUS3 genes in seed development and germination process was detected. TaFUS3-3B and TaFUS3-3D had similar expression profiles, and high levels of gene transcripts were detected in seeds at 25 DAP (days after pollination) and after 24 h of imbibition. However, the transcription of TaFUS3-3A was not detected. Silencing of TaFUS3 in common wheat spikes resulted in increased seed germination and PHS. Compared with wild-type, the TaFUS3-silenced plants showed increased expression of genes related to GA biosynthesis and ABA metabolism, and decreased expression of genes associated with ABA biosynthesis. Moreover, silencing of TaFUS3 in wheat plants led to a decrease in embryo sensitivity to ABA and changed the expression of genes involved in ABA signal transduction. The results of gene silencing indicated that TaFUS3 plays a positive role in wheat seed dormancy and PHS-resistance, which might be associated with ABA, GA level and signal transduction.”
Claim · model-written summary
TaFUS3 silencing changed ABA signal transduction gene expression.
- Taxon
- wheatTriticum aestivume1
- Treatment
- silencing of TaFUS3e1
- Outcome
- expression of genes involved in ABA signal transductione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“changed the expression of genes involved in ABA signal transduction”
e1“Pre-harvest sprouting (PHS) reduces yields and grain quality, resulting in seriously economic losses in wheat. It has been showed that PHS is significantly correlated to seed dormancy levels. FUSCA3 (FUS3) gene is considered to be the key regulator of seed dormancy. However, little information is available about the function of FUS3 gene (TaFUS3) in wheat. In this study, three homologous genes were identified in wheat grain, and their functions were investigated by gene silencing. Three full-length DNA (3477, 3534 and 3501 bp) and cDNA (1015, 1012 and 1015 bp) sequences encoding a B3 transcription factor, designated TaFUS3-3A, TaFUS3-3B and TaFUS3-3D, were first isolated from common wheat. The transcription of three TaFUS3 genes in seed development and germination process was detected. TaFUS3-3B and TaFUS3-3D had similar expression profiles, and high levels of gene transcripts were detected in seeds at 25 DAP (days after pollination) and after 24 h of imbibition. However, the transcription of TaFUS3-3A was not detected. Silencing of TaFUS3 in common wheat spikes resulted in increased seed germination and PHS. Compared with wild-type, the TaFUS3-silenced plants showed increased expression of genes related to GA biosynthesis and ABA metabolism, and decreased expression of genes associated with ABA biosynthesis. Moreover, silencing of TaFUS3 in wheat plants led to a decrease in embryo sensitivity to ABA and changed the expression of genes involved in ABA signal transduction. The results of gene silencing indicated that TaFUS3 plays a positive role in wheat seed dormancy and PHS-resistance, which might be associated with ABA, GA level and signal transduction.”
Claim · model-written summary
TaFUS3 was interpreted to promote dormancy and sprouting resistance.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaFUS3e0
- Outcome
- seed dormancy and PHS-resistancee0
- Qualifier
- might be associated with ABA, GA level and signal transductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaFUS3 plays a positive role in wheat seed dormancy and PHS-resistance, which might be associated with ABA, GA level and signal transduction”
e1“Pre-harvest sprouting (PHS) reduces yields and grain quality, resulting in seriously economic losses in wheat. It has been showed that PHS is significantly correlated to seed dormancy levels. FUSCA3 (FUS3) gene is considered to be the key regulator of seed dormancy. However, little information is available about the function of FUS3 gene (TaFUS3) in wheat. In this study, three homologous genes were identified in wheat grain, and their functions were investigated by gene silencing. Three full-length DNA (3477, 3534 and 3501 bp) and cDNA (1015, 1012 and 1015 bp) sequences encoding a B3 transcription factor, designated TaFUS3-3A, TaFUS3-3B and TaFUS3-3D, were first isolated from common wheat. The transcription of three TaFUS3 genes in seed development and germination process was detected. TaFUS3-3B and TaFUS3-3D had similar expression profiles, and high levels of gene transcripts were detected in seeds at 25 DAP (days after pollination) and after 24 h of imbibition. However, the transcription of TaFUS3-3A was not detected. Silencing of TaFUS3 in common wheat spikes resulted in increased seed germination and PHS. Compared with wild-type, the TaFUS3-silenced plants showed increased expression of genes related to GA biosynthesis and ABA metabolism, and decreased expression of genes associated with ABA biosynthesis. Moreover, silencing of TaFUS3 in wheat plants led to a decrease in embryo sensitivity to ABA and changed the expression of genes involved in ABA signal transduction. The results of gene silencing indicated that TaFUS3 plays a positive role in wheat seed dormancy and PHS-resistance, which might be associated with ABA, GA level and signal transduction.”
The same questions in other papers
loss of function · quality: postharvest · Triticum aestivum (wheat)Positive and negative results
6 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- no effect
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.