Abstract
Arsenic is one of the most dangerous metalloids, and silicon is a helpful element supporting plants to withstand stress. In this study, three factors were considered, including rice accessions with three different lines, including Lsi1-RNAi line (LE-R), Lsi1 overexpression line (LE-OE), and their wild type (LE-WT), and silicon and arsenic treatments with two different levels. Analysis of variance in dry weight biomass, protein content, arsenic, and silicon concentration has shown a significant interaction between three factors. Further analysis showed that the silicon concentration of all rice seedlings under silicon treatments increased significantly. The LE-OE line has shown a higher ability to absorb silicon in hydroponic conditions than the wild type, and when the seedlings were exposed to arsenic, the concentration of arsenic in all lines increased significantly. Adding silicon to over-expressed rice lines with the Lsi1 gene creates better arsenic resistance than their wild type. These findings confirmed antagonism between silicon and arsenic, and seedlings exposed to arsenic showed a reduction in silicon concentration in all rice lines. RNA-seq analysis showed 106 differentially expressed genes in the LE-OE line, including 75 up-regulated genes and 31 down-regulated genes. DEGs in the LE-R line were 449 genes, including 190 up-regulated and 259 down-regulated genes. Adding treatment has changed the expression of Calcium-binding EGF domain-containing, Os10g0530500, Os05g0240200 in both LE-OE and LE-R roots. They showed that transgenic cultivars were more resistant to arsenic than wild-type, especially when silicon was added to the culture medium.
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Claims 14
Claim · model-written summary
The three factors interacted significantly for biomass, protein, arsenic and silicon concentrations.
- Taxon
- rice
Oryza sativae1 - Treatment
- silicon and arsenic treatmentse1
- Outcome
- dry weight biomass, protein content, arsenic, and silicon concentratione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of variance in dry weight biomass, protein content, arsenic, and silicon concentration has shown a significant interaction between three factors”
e1“rice accessions with three different lines, including Lsi1-RNAi line (LE-R), Lsi1 overexpression line (LE-OE), and their wild type (LE-WT), and silicon and arsenic treatments”
Claim · model-written summary
Silicon treatment significantly increased seedling silicon concentration.
- Taxon
- riceOryza sativae0
- Treatment
- silicon treatmentse0
- Outcome
- silicon concentratione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the silicon concentration of all rice seedlings under silicon treatments increased significantly”
Claim · model-written summary
LE-OE absorbed more silicon than wild type in hydroponics.
- Taxon
- riceOryza sativae1
- Material
- LE-OE linee0
- Outcome
- ability to absorb silicone0
- Direction
- highere0
- Comparator
- wild typee0
- Setting
- hydroponic conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The LE-OE line has shown a higher ability to absorb silicon in hydroponic conditions than the wild type”
e1“rice accessions”
Claim · model-written summary
Arsenic exposure significantly increased arsenic concentration in all lines.
- Taxon
- riceOryza sativae1
- Treatment
- exposed to arsenice0
- Outcome
- concentration of arsenice0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when the seedlings were exposed to arsenic, the concentration of arsenic in all lines increased significantly”
e1“rice accessions”
Claim · model-written summary
Added silicon gave overexpressing lines better arsenic resistance than wild type.
- Taxon
- riceOryza sativae0
- Treatment
- Adding silicone0
- Gene
- Lsi1e0
- Outcome
- arsenic resistancee0
- Direction
- bettere0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Adding silicon to over-expressed rice lines with the Lsi1 gene creates better arsenic resistance than their wild type”
Claim · model-written summary
Arsenic exposure reduced silicon concentration in all rice lines.
- Taxon
- riceOryza sativae0
- Treatment
- exposed to arsenice0
- Outcome
- silicon concentratione0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seedlings exposed to arsenic showed a reduction in silicon concentration in all rice lines”
Claim · model-written summary
LE-OE had 106 differentially expressed genes.
- Taxon
- riceOryza sativae1
- Material
- LE-OE linee0
- Outcome
- differentially expressed genese0
- Value
- 106e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA-seq analysis showed 106 differentially expressed genes in the LE-OE line, including 75 up-regulated genes and 31 down-regulated genes”
e1“rice accessions”
Claim · model-written summary
LE-OE had 75 up-regulated genes.
- Taxon
- riceOryza sativae2
- Material
- LE-OE linee1
- Outcome
- up-regulated genese0
- Value
- 75e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“including 75 up-regulated genes and 31 down-regulated genes”
e1“RNA-seq analysis showed 106 differentially expressed genes in the LE-OE line”
e2“rice accessions”
Claim · model-written summary
LE-OE had 31 down-regulated genes.
- Taxon
- riceOryza sativae2
- Material
- LE-OE linee1
- Outcome
- down-regulated genese0
- Value
- 31e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“including 75 up-regulated genes and 31 down-regulated genes”
e1“RNA-seq analysis showed 106 differentially expressed genes in the LE-OE line”
e2“rice accessions”
Claim · model-written summary
LE-R had 449 differentially expressed genes.
- Taxon
- riceOryza sativae1
- Material
- LE-R linee0
- Outcome
- DEGse0
- Value
- 449 genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DEGs in the LE-R line were 449 genes, including 190 up-regulated and 259 down-regulated genes”
e1“rice accessions”
Claim · model-written summary
LE-R had 190 up-regulated genes.
- Taxon
- riceOryza sativae1
- Material
- LE-R linee0
- Outcome
- up-regulatede0
- Value
- 190e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DEGs in the LE-R line were 449 genes, including 190 up-regulated and 259 down-regulated genes”
e1“rice accessions”
Claim · model-written summary
LE-R had 259 down-regulated genes.
- Taxon
- riceOryza sativae1
- Material
- LE-R linee0
- Outcome
- down-regulated genese0
- Value
- 259e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DEGs in the LE-R line were 449 genes, including 190 up-regulated and 259 down-regulated genes”
e1“rice accessions”
Claim · model-written summary
Treatment changed expression of three named genes in both root lines.
- Taxon
- riceOryza sativae1
- Organ / stage
- rootse0
- Gene
- Calcium-binding EGF domain-containing, Os10g0530500, Os05g0240200e0
- Outcome
- expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Adding treatment has changed the expression of Calcium-binding EGF domain-containing, Os10g0530500, Os05g0240200 in both LE-OE and LE-R roots”
e1“rice accessions”
Claim · model-written summary
Transgenic cultivars were more arsenic resistant than wild type, especially with silicon.
- Taxon
- riceOryza sativae1
- Material
- transgenic cultivarse0
- Outcome
- resistant to arsenice0
- Direction
- moree0
- Qualifier
- especially when silicon was added to the culture mediume0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic cultivars were more resistant to arsenic than wild-type, especially when silicon was added to the culture medium”
e1“rice accessions”
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