Abstract
High temperature negatively affects the plants. In particular, under the heat stress he production of reactive oxygen species increases in the plant cell. It leads to the development of oxidative stress. The formation of carbonyl groups in proteins is a marker of oxidative damage of plant cells. Plants have a defense system that consists of soluble carbohydrates such as sucrose and glucose and antioxidant enzymes, including catalase. Carbohydrates have protective mechanisms and can activate different signaling pathways with following changes in gene expression. Despite the data available, information on the effects of sucrose and glucose on the oxidative modification of proteins under heat stress is insufficient. The aim of our work was to study the role of sucrose and glucose for the carbonyl groups content in cat2cat3 knockout plants of A. thaliana under heat stress. We used 7-week-old Arabidopsis thaliana plants of wild-type and knockout cat2cat3 line, which lacks the expression of two catalase genes – cat2 and cat3. Plants were grown under 16-hour light day at a temperature of + 20°C and an illumination of 2.5 kL. Heat treatment was performed on a water bath in glass flasks with 15-20 leaves which were incubated in 1 mm potassium phosphate buffer without carbohydrates and with addition of sucrose or glucose (1% final concentration) during 2 and 4 hours at the +37°C and +44° C. The content of carbonyl groups and total protein was determined photometrically. It has been shown that intact knockout cat2cat3 plants have a higher content of carbonyl groups, which indicates chronic oxidative stress. Addition of exogenous sucrose or glucose to the incubation buffer had a protective effect during 4 hours of stress. Carbonyl groups formation in wild type decreased under the +37ºС and +44ºС, while in the cat2сat3 line only under the moderate (+37ºС) heat stress. In the knockout mutant alternative ways of defense are exhausted under +44ºC.
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Claims 6
Claim · model-written summary
Intact cat2cat3 knockout plants had more protein carbonyl groups, indicating chronic oxidative stress.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Material
- cat2cat3e0
- Outcome
- content of carbonyl groupse0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“intact knockout cat2cat3 plants have a higher content of carbonyl groups, which indicates chronic oxidative stress.”
t0“Arabidopsis thaliana cat2cat3 knockout mutant”
Claim · model-written summary
Exogenous sucrose protected plants during four hours of heat stress.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- exogenous sucrosee0
- Outcome
- protective effecte0
- Qualifier
- 4 hours of stresse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of exogenous sucrose or glucose to the incubation buffer had a protective effect during 4 hours of stress.”
t0“Arabidopsis thaliana cat2cat3 knockout mutant”
Claim · model-written summary
Exogenous glucose protected plants during four hours of heat stress.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- glucosee0
- Outcome
- protective effecte0
- Qualifier
- 4 hours of stresse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of exogenous sucrose or glucose to the incubation buffer had a protective effect during 4 hours of stress.”
t0“Arabidopsis thaliana cat2cat3 knockout mutant”
Claim · model-written summary
Carbonyl group formation decreased in wild type at +37ºС and +44ºС.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- wild typee0
- Treatment
- +37ºС and +44ºСe0
- Outcome
- Carbonyl groups formatione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Carbonyl groups formation in wild type decreased under the +37ºС and +44ºС”
t0“Arabidopsis thaliana cat2cat3 knockout mutant”
Claim · model-written summary
Carbonyl group formation in cat2сat3 decreased only under moderate +37ºС heat stress.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- cat2сat3e0
- Treatment
- moderate (+37ºС) heat stresse0
- Outcome
- Carbonyl groups formatione1
- Direction
- decreasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the cat2сat3 line only under the moderate (+37ºС) heat stress.”
e1“Carbonyl groups formation in wild type decreased under the +37ºС and +44ºС”
t0“Arabidopsis thaliana cat2cat3 knockout mutant”
Claim · model-written summary
The knockout mutant exhausted alternative defenses at +44ºC.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- knockout mutante0
- Outcome
- alternative ways of defense are exhaustede0
- Qualifier
- under +44ºCe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the knockout mutant alternative ways of defense are exhausted under +44ºC.”
t0“Arabidopsis thaliana cat2cat3 knockout mutant”
The same questions in other papers
heat · molecular: metabolites · Arabidopsis thaliana (thale cress)Positive and negative results
2 papers in the same direction as this paper · 5 in the opposite direction
- no effect
- negative
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.