Abstract
Sweet potato (lpomoea batatas) is a type of dicotyledon plant that was consumed as an important crop in several countries such as Africa and China. However, most of people only use up the tuber of sweet potato but the other parts such as leaves and stems are usually discarded. Based on previous study, the stems of sweet potato plant actually contain secondary metabolite. It is related to a research conducted by Muhamad Ridzuan (2017) that was found the presented of unknown alkaloid in stems of wild sweet potato plant. As reported, alkaloid may be used in treatment of ovarian cancer. Therefore, the comparison of alkaloid that presented within the callus induced from this study and the wild type plant will be done. The purpose of this research is to induce the callus from stem of sweet potato by using different types of hormones such as 2,4-dichlorophenoxyacetic acid (2,4-D), napthaleneacetic acid (NAA) and indole-3-acetic acid (IAA) at different concentrations. Other than that, the aim of this study is to compare the callus induction from stem of sweet potato by different types of phytohormone such as, 2,4-dichlorophenoxyacetic acid (2,4-D), napthaleneacetic acid (NAA) and indole- 3-acetic acid (IAA) at different concentrations (1.0, 2.0, 3.0, 4.0 and 5.0 mg/L). The explants were cultured in media containing of phytohormone and was observed in 30 days. Results obtained shown that the best hormone induced callus was NAA, followed by 2,4-D. Unfortunately, there was no callus induced through lAA. Based on concentration, the highest percentage callus was induced by NAA at 1.0 and 4.0 mg/L (100%). Meanwhile, the percentage of callus induced by 2,4-D was at 5.0 mg/L concentration (6.67%). For the narrowed down concentration of 2,4-D, the highest percentage of callus (66.67%) was induced at concentration 1.8 mg/L. After 4 days, yellowish colour of callus was obtained from the sample supplemented with all phytohormones. According to the result, the presented of secondary metabolites such as alkaloid can be detected through Thin Layer Chromatography (TLC) from the callus induced. Therefore, the optimization of callus growth by using different media and formulation of concentration phytohormone can be done. In addition, the concentration of the alkaloid can be obtained through High Performance Liquid Chromatography (HPLC).
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Claims 7
Claim · model-written summary
NAA was the best hormone for callus induction, followed by 2,4-D.
- Taxon
- sweet potato (Ipomoea batatas)
_Flower.jpg?width=60)
Ipomoea batatast0 - Treatment
- NAAe0
- Outcome
- induced calluse0
- Direction
- beste0
- Comparator
- 2,4-De0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Flower.jpg?width=500)
Verbatim
e0“the best hormone induced callus was NAA, followed by 2,4-D”
t0“sweet potato (Ipomoea batatas)”
Claim · model-written summary
IAA did not induce callus.
- Taxon
- sweet potato (Ipomoea batatas)Ipomoea batatast0
- Treatment
- lAAe0
- Outcome
- callus inducede0
- Direction
- noe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was no callus induced through lAA”
t0“sweet potato (Ipomoea batatas)”
Claim · model-written summary
NAA at 1.0 and 4.0 mg/L induced the highest callus percentage, 100%.
- Taxon
- sweet potato (Ipomoea batatas)Ipomoea batatast0
- Treatment
- NAA at 1.0 and 4.0 mg/Le0
- Outcome
- percentage calluse0
- Direction
- higheste0
- Value
- 100%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest percentage callus was induced by NAA at 1.0 and 4.0 mg/L (100%)”
t0“sweet potato (Ipomoea batatas)”
Claim · model-written summary
At 5.0 mg/L, 2,4-D induced 6.67% callus.
- Taxon
- sweet potato (Ipomoea batatas)Ipomoea batatast0
- Treatment
- 2,4-D was at 5.0 mg/Le0
- Outcome
- percentage of calluse0
- Value
- 6.67%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the percentage of callus induced by 2,4-D was at 5.0 mg/L concentration (6.67%)”
t0“sweet potato (Ipomoea batatas)”
Claim · model-written summary
At 1.8 mg/L, 2,4-D induced the highest callus percentage, 66.67%, in the narrowed concentration range.
- Taxon
- sweet potato (Ipomoea batatas)Ipomoea batatast0
- Treatment
- 2,4-De1
- Outcome
- percentage of calluse0
- Direction
- higheste0
- Value
- 66.67%e0
- Qualifier
- concentration 1.8 mg/Le0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest percentage of callus (66.67%) was induced at concentration 1.8 mg/L”
e1“For the narrowed down concentration of 2,4-D”
t0“sweet potato (Ipomoea batatas)”
Claim · model-written summary
Callus from all phytohormone-supplemented samples was yellowish after four days.
- Taxon
- sweet potato (Ipomoea batatas)Ipomoea batatast0
- Treatment
- all phytohormonese0
- Outcome
- yellowish colour of calluse0
- Qualifier
- After 4 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 4 days, yellowish colour of callus was obtained from the sample supplemented with all phytohormones”
t0“sweet potato (Ipomoea batatas)”
Claim · model-written summary
TLC detected alkaloid in induced callus.
- Taxon
- sweet potato (Ipomoea batatas)Ipomoea batatast0
- Organ / stage
- calluse0
- Outcome
- alkaloid can be detectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“secondary metabolites such as alkaloid can be detected through Thin Layer Chromatography (TLC) from the callus induced”
t0“sweet potato (Ipomoea batatas)”
The same questions in other papers
2,4-D · growth · Ipomoea batatas (sweet potato)Positive and negative results
3 papers in the same direction as this paper · 3 in the opposite direction
- negative
- no effect
- negative
3 papers more in this group
See all 7 papers →1-naphthaleneacetic acid · growth · Ipomoea batatas (sweet potato)Positive and negative results
10 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.