Abstract
Carbon and nitrogen antagonistically regulate multiple developmental processes. However, the molecular mechanism affecting nitrogen metabolism by sucrose transport remains poorly defined. Previously, we noted that Oryza sativa DNA BINDING WITH ONE FINGER 11 ( OsDOF11 ) mediated sucrose transport by binding to the promoter regions of Sucrose Transporter 1 ( SUT1 ), Oryza sativa Sugars Will Eventually be Exported Transporters 11 (OsSWEET11) , and OsSWEET14 . Here, we note that OsDOF11 promotes nitrogen uptake and then maintains the ratio of fresh weight to dry weight in seedling plants and the effective leaf blade at flowering stages. Mutants of the sucrose transporter gene OsSWEET14 displayed a phenotype similar to that of OsDOF11 . By microarray analysis and qRT-PCR in OsDOF11 mutant plants, OsDOF11 affected the transcription level of amino acid metabolism-related genes. We further found that mainly amino acid contents were reduced in flag leaves but increased in seeds. Both sugar and organic nitrogen changes caused the ratio of fresh weight to dry weight to decrease in OsDOF11 mutant seedling plants and mature leaves, which might result in vigorous reduced metabolic activity and become less susceptible to stress. These results demonstrated that OsDOF11 affected nitrogen metabolism by sugar distribution in rice, which provided new insight that OsDOF11 coordinated with C and N balance to maintain plant growth activity.
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Claims 8
Claim · model-written summary
OsDOF11 promoted nitrogen uptake in seedlings and flowering leaf blades.
- Taxon
- Rice
Oryza sativat0 - Organ / stage
- seedling plants and the effective leaf blade at flowering stagese0
- Gene
- OsDOF11e0
- Outcome
- nitrogen uptakee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsDOF11 promotes nitrogen uptake and then maintains the ratio of fresh weight to dry weight in seedling plants and the effective leaf blade at flowering stages”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
OsDOF11 maintained the fresh to dry weight ratio.
- Taxon
- RiceOryza sativat0
- Organ / stage
- seedling plants and the effective leaf blade at flowering stagese0
- Gene
- OsDOF11e0
- Outcome
- ratio of fresh weight to dry weighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsDOF11 promotes nitrogen uptake and then maintains the ratio of fresh weight to dry weight in seedling plants and the effective leaf blade at flowering stages”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
OsSWEET14 mutants had a phenotype similar to OsDOF11 mutants.
- Taxon
- RiceOryza sativat0
- Gene
- OsSWEET14e0
- Outcome
- phenotype similar to that of OsDOF11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutants of the sucrose transporter gene OsSWEET14 displayed a phenotype similar to that of OsDOF11”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
OsDOF11 affected transcription of amino acid metabolism genes.
- Taxon
- RiceOryza sativat0
- Gene
- OsDOF11e0
- Outcome
- transcription level of amino acid metabolism-related genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsDOF11 affected the transcription level of amino acid metabolism-related genes”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
Amino acid contents were reduced in flag leaves.
- Taxon
- RiceOryza sativat0
- Organ / stage
- flag leavese0
- Outcome
- amino acid contentse0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mainly amino acid contents were reduced in flag leaves but increased in seeds”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
Amino acid contents increased in seeds.
- Taxon
- RiceOryza sativat0
- Organ / stage
- seedse0
- Outcome
- amino acid contentse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“amino acid contents were reduced in flag leaves but increased in seeds”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
Sugar and organic nitrogen changes decreased the fresh to dry weight ratio in OsDOF11 mutants.
- Taxon
- RiceOryza sativat0
- Gene
- OsDOF11e0
- Outcome
- ratio of fresh weight to dry weighte0
- Direction
- decreasee0
- Qualifier
- sugar and organic nitrogen changese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both sugar and organic nitrogen changes caused the ratio of fresh weight to dry weight to decrease in OsDOF11 mutant seedling plants and mature leaves”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
The authors suggested reduced metabolic activity and stress susceptibility in OsDOF11 mutants.
- Taxon
- RiceOryza sativat0
- Gene
- OsDOF11e1
- Outcome
- reduced metabolic activity and become less susceptible to stresse0
- Qualifier
- might resulte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which might result in vigorous reduced metabolic activity and become less susceptible to stress”
e1“OsDOF11 mutant seedling plants and mature leaves”
t0“Rice (Oryza sativa L.)”