Abstract
The plant cell wall (CW) is an outer cell skeleton that plays an important role in plant growth and protection against both biotic and abiotic stresses. Signals and molecules produced during host–pathogen interactions have been proven to be involved in plant stress responses initiating signal pathways. Based on our previous research findings, the present study explored the possibility of additively or synergistically increasing plant stress resistance by stacking beneficial genes. In order to prove our hypothesis, we generated transgenic Arabidopsis plants constitutively overexpressing three different Aspergillus nidulans CW-modifying enzymes: a xylan acetylesterase, a rhamnogalacturonan acetylesterase and a feruloylesterase. The two acetylesterases were expressed either together or in combination with the feruloylesterase to study the effect of CW polysaccharide deacetylation and deferuloylation on Arabidopsis defense reactions against a fungal pathogen, Botrytis cinerea. The transgenic Arabidopsis plants expressing two acetylesterases together showed higher CW deacetylation and increased resistance to B. cinerea in comparison to wild-type (WT) Col-0 and plants expressing single acetylesterases. While the expression of feruloylesterase alone compromised plant resistance, coexpression of feruloylesterase together with either one of the two acetylesterases restored plant resistance to the pathogen. These CW modifications induced several defense-related genes in uninfected healthy plants, confirming their impact on plant resistance. These results demonstrated that coexpression of complementary CW-modifying enzymes in different combinations have an additive effect on plant stress response by constitutively priming the plant defense pathways. These findings might be useful for generating valuable crops with higher protections against biotic stresses.
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Claims 6
Claim · model-written summary
Coexpressing two acetylesterases increased cell wall deacetylation versus wild type and single enzyme lines.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- expressing two acetylesterases togethere0
- Interactor
- B. cinerea
Botrytis cinereae0pathogen - Outcome
- CW deacetylatione0
- Direction
- highere0
- Comparator
- wild-type (WT) Col-0 and plants expressing single acetylesterasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic Arabidopsis plants expressing two acetylesterases together showed higher CW deacetylation and increased resistance to B. cinerea in comparison to wild-type (WT) Col-0 and plants expressing single acetylesterases”
Claim · model-written summary
Coexpressing two acetylesterases increased resistance to Botrytis versus wild type and single enzyme lines.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- expressing two acetylesterases togethere0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Outcome
- resistance to B. cinereae0
- Direction
- increasede0
- Comparator
- wild-type (WT) Col-0 and plants expressing single acetylesterasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic Arabidopsis plants expressing two acetylesterases together showed higher CW deacetylation and increased resistance to B. cinerea in comparison to wild-type (WT) Col-0 and plants expressing single acetylesterases”
Claim · model-written summary
Feruloylesterase expression alone compromised Arabidopsis resistance.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- expression of feruloylesterase alonee0
- Interactor
- Botrytis cinereaBotrytis cinereae1pathogen
- Outcome
- plant resistancee0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of feruloylesterase alone compromised plant resistance”
e1“Arabidopsis defense reactions against a fungal pathogen, Botrytis cinerea”
Claim · model-written summary
Feruloylesterase coexpression with either acetylesterase restored resistance.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- coexpression of feruloylesterase together with either one of the two acetylesterasese0
- Interactor
- Botrytis cinereaBotrytis cinereae1pathogen
- Outcome
- plant resistance to the pathogene0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“coexpression of feruloylesterase together with either one of the two acetylesterases restored plant resistance to the pathogen”
e1“Arabidopsis defense reactions against a fungal pathogen, Botrytis cinerea”
Claim · model-written summary
Cell wall modifications induced several defense related genes in uninfected plants.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- CW modificationse0
- Outcome
- defense-related genese0
- Direction
- inducede0
- Qualifier
- uninfected healthy plantse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These CW modifications induced several defense-related genes in uninfected healthy plants”
e1“transgenic Arabidopsis plants expressing”
Claim · model-written summary
The authors interpret combined enzyme expression as additively priming plant defense.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- coexpression of complementary CW-modifying enzymese0
- Outcome
- plant stress responsee0
- Qualifier
- constitutively priming the plant defense pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“coexpression of complementary CW-modifying enzymes in different combinations have an additive effect on plant stress response by constitutively priming the plant defense pathways”
e1“transgenic Arabidopsis plants expressing”
The same questions in other papers
transgenic · Botrytis cinerea · ArabidopsisPositive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
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