Paper · 2021 · Acta Scientific Agriculture

Callus Induction from Leaf and Stem Explants of Selected Turkish Genotypes of Common Bean

Asli Küçükrecep; Dilek Tekdal

2
claims
4
verbatim quotes
3
citations
1
research areas
✓
Open access

Abstract

Common bean (Phaseolus vulgaris L.), a species belonging to the family Fabaceae, is one of the most critical legumes consumed and grown globally. Common bean ranks first among grain legumes in terms of cultivation area and production in the world. However, the bean is recalcitrant, making obtaining this plant via in vitro culture techniques a challenge. Due to changing climatic factors and drought conditions that may happen in the future, it is essential to produce plants that make up human food in vitro conditions. With this goal, the effect of different concentrations of 2,4-D and Kinetin and their possible combinations on callus induction in leaf and stem explants of three-bean genotypes (Bitlis117, Gynk, and nceler) were evaluated. It aimed to determine the best medium that promotes callus formation in different bean genotypes and tissue types. Callogenesis was successfully promoted from leaf and stem explants of all three genotypes tested. The highest callus ratio was obtained from stem explants of Bitlis117 cultured on the MS medium, including 2,4-D (0.5mg L -1 ) and Kinetin (2.0 mg L -1 ).

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Claims 2

Observationobservedoriginal resultmeasured

Claim · model-written summary

Callogenesis was successfully promoted from leaf and stem explants of all three bean genotypes.

Taxon
Common BeanPhaseolus vulgarist0
Organ / stage
leaf and stem explantse2
Outcome
Callogenesise2
Direction
successfully promotede2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Common Bean”

✓ located verbatim in the source · title, characters 78–89 · show in text ↑
e2

“Callogenesis was successfully promoted from leaf and stem explants of all three genotypes tested”

✓ located verbatim in the source · abstract, characters 861–957 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Stem explants of Bitlis117 on MS medium with 2,4-D and kinetin gave the highest callus ratio.

Taxon
Common BeanPhaseolus vulgarist0
Material
Bitlis117e4
Organ / stage
stem explantse4
Treatment
MS medium, including 2,4-D (0.5mg L -1 ) and Kinetin (2.0 mg L -1 )e4
Outcome
callus ratioe4
Direction
higheste4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Common Bean”

✓ located verbatim in the source · title, characters 78–89 · show in text ↑
e4

“The highest callus ratio was obtained from stem explants of Bitlis117 cultured on the MS medium, including 2,4-D (0.5mg L -1 ) and Kinetin (2.0 mg L -1 )”

✓ located verbatim in the source · abstract, characters 959–1112 · show in text ↑

2,4-D · growth · Phaseolus vulgaris (common bean)Results differ

18 papers: 9 positive, 9 negative or no effect

8 papers in the same direction as this paper · 9 in the opposite direction

  1. negative
    Only 2,4-D changed chloroplast morphology and internal structure after 24 h, and only in light.

    The Effect of 2,4-Dichlorophenoxyacetic Acid and Related Compounds on the Fine Structure of the Primary Leaves ofPhaseolus vulgaris · 1970 · compared with 2-chlorophenoxyacetic acid (2-CPA), 4-chlorophenoxyacetic acid (4-CPA), 2,6-dichlorophenoxyacetic acid (2,6-D)

    Opposite direction

  2. negative
  3. negative

14 papers more in this group

See all 18 papers →

kinetin · growth · Phaseolus vulgaris (common bean)Positive and negative results

9 papers: 6 positive, 3 negative or no effect

5 papers in the same direction as this paper · 3 in the opposite direction

  1. negative
  2. negative
  3. no effect

5 papers more in this group

See all 9 papers →

in vitro · growth · Phaseolus vulgaris (common bean)Positive and negative results

18 papers: 11 positive, 7 negative or no effect

10 papers in the same direction as this paper · 7 in the opposite direction

  1. negative
  2. negative
  3. negative

14 papers more in this group

See all 18 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.