Abstract
Plumule of four rice varieties viz., TRY 2, TRY 3, ADT 53 and CO 52 were studied for callus induction and regeneration using different concentrations and combinations of plant growth regulators in MS medium. The combination of MS medium with 2,4-D (2.0 mg/l) + Kn (0.5 mg/l) showed maximum callus induction frequency in all four varieties ranged from 93.33 to 76.67 compared to other growth regulator combinations. Upon subculture of the callus, MS medium with NAA (0.5 mg/l) + BAP (2.0 mg/l) was found to be superior for greening of calli and regeneration of shoots, followed by MS + NAA (0.5 mg/l) + BAP (1.5 mg/l). Among the four varieties TRY 2 and TRY 3 showed positive responses for regeneration.
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Claims 3
Claim · model-written summary
MS medium with 2,4-D (2.0 mg/l) and Kn (0.5 mg/l) gave the maximum callus induction frequency across four varieties, ranging from 93.33 to 76.67, versus other regulator combinations.
- Taxon
- rice
Oryza sativat0 - Treatment
- MS medium with 2,4-D (2.0 mg/l) + Kn (0.5 mg/l)e0
- Outcome
- callus induction frequencye0
- Direction
- maximume0
- Value
- 93.33 to 76.67e0
- Comparator
- other growth regulator combinationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The combination of MS medium with 2,4-D (2.0 mg/l) + Kn (0.5 mg/l) showed maximum callus induction frequency in all four varieties ranged from 93.33 to 76.67 compared to other growth regulator combinations”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
MS medium with NAA (0.5 mg/l) and BAP (2.0 mg/l) was superior for callus greening and shoot regeneration to the 1.5 mg/l BAP combination.
- Taxon
- riceOryza sativat0
- Treatment
- MS medium with NAA (0.5 mg/l) + BAP (2.0 mg/l)e0
- Outcome
- greening of calli and regeneration of shootse0
- Direction
- superiore0
- Comparator
- MS + NAA (0.5 mg/l) + BAP (1.5 mg/l)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium with NAA (0.5 mg/l) + BAP (2.0 mg/l) was found to be superior for greening of calli and regeneration of shoots, followed by MS + NAA (0.5 mg/l) + BAP (1.5 mg/l)”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
TRY 2 and TRY 3 responded positively to regeneration.
- Taxon
- riceOryza sativat0
- Material
- TRY 2 and TRY 3e0
- Outcome
- positive responses for regeneratione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among the four varieties TRY 2 and TRY 3 showed positive responses for regeneration”
t0“rice (Oryza sativa L.)”
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