Abstract
Of 10 indica rice varieties assessed, Annada showed the best in vitro culture response in respect of callus induction, plantlet regeneration and number of plantlets/seed callus. Other high yielding varieties, viz. Kasturi, IR 72 and Karnal Local, also displayed appreciable performance. Callus induced and proliferated in dark and gave high plantlet regeneration and more plantlets/seed callus across genotypes. Among three shock treatments, viz. heat (37 °C for overnight), cold (4 °C for over night) and dehydration, dehydration significantly enhanced the plantlet regeneration and number of plantlets/seed callus. Of 3-dehydration treatments (6, 12 and 24 h duration), the maximum plantlet regeneration and number of plantlets/seed callus were observed in the calli dehydrated for 12 h. Of three tested media, viz. MS, LS, and N 6, MS emerged the best for callus induction, plantlet regeneration and number of plantlets/seed callus. In contrast, among the modified media formulations, modified N6 [5 m M (NH 4)2 SO 4] produced maximum plantlets/seed callus across varieties. Increased (5 mM) (NH 4)2SO 4 content in the medium was found to be better than the higher dose of KNO 3 or its normal level in the respective media formulations. Across genotypes, 2 mg L -1 2,4-D in the callus induction medium (CIM) was the most suitable for callus induction. However, 2,4-D in combination with other growth regulators, such as 2 mg L -1 2,4-D and 1 mg L -1 Kn, performed better. In regeneration medium (RM), 2 mg L -1 BAP with 1 mg L -1 Kn and 0.5 mg L -1 NAA, or 2 mg L -1 BAP with 0.5 mg L -1 NAA, were optimum for the maximum plantlet regeneration. Across varieties, coconut water (10%) was found to be the most effective to enhance the in vitro culture response as evident from prolific callus induction, increased plantlet regeneration and more plantlets/seed callus.
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Claims 15
Claim · model-written summary
Of 10 indica rice varieties, Annada showed the best in vitro culture response for callus induction, plantlet regeneration and number of plantlets per seed callus.
- Taxon
- indica rice
Oryza sativa Indica Groupt0 - Material
- Annadae1
- Outcome
- callus induction, plantlet regeneration and number of plantlets/seed calluse1
- Direction
- beste1
- Comparator
- 10 indica rice varietiese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice”
e1“Of 10 indica rice varieties assessed, Annada showed the best in vitro culture response in respect of callus induction, plantlet regeneration and number of plantlets/seed callus”
Claim · model-written summary
The high yielding variety Kasturi also displayed appreciable in vitro performance.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Material
- Kasturie1
- Outcome
- appreciable performancee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice”
e1“Other high yielding varieties, viz. Kasturi, IR 72 and Karnal Local, also displayed appreciable performance”
Claim · model-written summary
The high yielding variety IR 72 also displayed appreciable in vitro performance.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Material
- IR 72e1
- Outcome
- appreciable performancee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice”
e1“Other high yielding varieties, viz. Kasturi, IR 72 and Karnal Local, also displayed appreciable performance”
Claim · model-written summary
The high yielding variety Karnal Local also displayed appreciable in vitro performance.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Material
- Karnal Locale1
- Outcome
- appreciable performancee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice”
e1“Other high yielding varieties, viz. Kasturi, IR 72 and Karnal Local, also displayed appreciable performance”
Claim · model-written summary
Callus induced and proliferated in the dark gave high plantlet regeneration and more plantlets per seed callus across genotypes.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- in darke1
- Outcome
- plantlet regeneration and more plantlets/seed calluse1
- Direction
- highe1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice”
e1“Callus induced and proliferated in dark and gave high plantlet regeneration and more plantlets/seed callus across genotypes”
Claim · model-written summary
Among heat, cold and dehydration shock treatments, dehydration significantly enhanced plantlet regeneration and number of plantlets per seed callus.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- dehydratione1
- Outcome
- plantlet regeneration and number of plantlets/seed calluse1
- Direction
- enhancede1
- Comparator
- heat (37 °C for overnight), cold (4 °C for over night)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice”
e1“Among three shock treatments, viz. heat (37 °C for overnight), cold (4 °C for over night) and dehydration, dehydration significantly enhanced the plantlet regeneration and number of plantlets/seed callus”
Claim · model-written summary
Among 6, 12 and 24 h dehydration treatments, calli dehydrated for 12 h gave the maximum plantlet regeneration and number of plantlets per seed callus.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- dehydrated for 12 he1
- Outcome
- plantlet regeneration and number of plantlets/seed calluse1
- Direction
- maximume1
- Comparator
- 3-dehydration treatmentse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice”
e1“Of 3-dehydration treatments (6, 12 and 24 h duration), the maximum plantlet regeneration and number of plantlets/seed callus were observed in the calli dehydrated for 12 h”
Claim · model-written summary
Of the MS, LS and N6 media, MS was the best for callus induction, plantlet regeneration and number of plantlets per seed callus.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- MSe1
- Outcome
- callus induction, plantlet regeneration and number of plantlets/seed calluse1
- Direction
- beste1
- Comparator
- LS, and N 6e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice”
e1“Of three tested media, viz. MS, LS, and N 6, MS emerged the best for callus induction, plantlet regeneration and number of plantlets/seed callus”
Claim · model-written summary
Among modified media formulations, modified N6 with 5 mM (NH4)2SO4 produced the maximum plantlets per seed callus across varieties.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- modified N6 [5 m M (NH 4)2 SO 4]e1
- Outcome
- plantlets/seed calluse1
- Direction
- maximume1
- Comparator
- modified media formulationse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice”
e1“among the modified media formulations, modified N6 [5 m M (NH 4)2 SO 4] produced maximum plantlets/seed callus across varieties”
Claim · model-written summary
Increased (5 mM) (NH4)2SO4 content in the medium was better than the higher dose of KNO3 or its normal level in the respective media formulations.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- Increased (5 mM) (NH 4)2SO4 contente1
- Outcome
- bettere1
- Direction
- bettere1
- Comparator
- higher dose of KNO 3 or its normal levele1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice”
e1“Increased (5 mM) (NH 4)2SO4 content in the medium was found to be better than the higher dose of KNO 3 or its normal level in the respective media formulations”
Claim · model-written summary
Across genotypes, 2 mg L-1 2,4-D in the callus induction medium was the most suitable for callus induction.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- 2 mg L -1 2,4-De1
- Outcome
- callus inductione1
- Direction
- most suitablee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice”
e1“Across genotypes, 2 mg L -1 2,4-D in the callus induction medium (CIM) was the most suitable for callus induction”
Claim · model-written summary
2,4-D in combination with other growth regulators, such as 2 mg L-1 2,4-D and 1 mg L-1 Kn, performed better.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- 2 mg L -1 2,4-D and 1 mg L -1 Kne1
- Outcome
- performed bettere1
- Direction
- performed bettere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice”
e1“2,4-D in combination with other growth regulators, such as 2 mg L -1 2,4-D and 1 mg L -1 Kn, performed better”
Claim · model-written summary
In regeneration medium, 2 mg L-1 BAP with 1 mg L-1 Kn and 0.5 mg L-1 NAA was optimum for maximum plantlet regeneration.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- 2 mg L -1 BAP with 1 mg L -1 Kn and 0.5 mg L -1 NAAe1
- Outcome
- maximum plantlet regeneratione1
- Direction
- optimume1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice”
e1“2 mg L -1 BAP with 1 mg L -1 Kn and 0.5 mg L -1 NAA, or 2 mg L -1 BAP with 0.5 mg L -1 NAA, were optimum for the maximum plantlet regeneration”
Claim · model-written summary
In regeneration medium, 2 mg L-1 BAP with 0.5 mg L-1 NAA was optimum for maximum plantlet regeneration.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- 2 mg L -1 BAP with 0.5 mg L -1 NAAe1
- Outcome
- maximum plantlet regeneratione1
- Direction
- optimume1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice”
e1“2 mg L -1 BAP with 0.5 mg L -1 NAA, were optimum for the maximum plantlet regeneration”
Claim · model-written summary
Across varieties, coconut water (10%) was the most effective in enhancing the in vitro culture response, with prolific callus induction, increased plantlet regeneration and more plantlets per seed callus.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- coconut water (10%)e1
- Outcome
- in vitro culture responsee1
- Direction
- most effectivee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice”
e1“coconut water (10%) was found to be the most effective to enhance the in vitro culture response”
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