Abstract
Enhancement of callus induction and its regeneration efficiency through in vitro techniques has been optimized for 2 abiotic stresses (salt and air desiccation) using 3 rice genotypes viz. BR10, BRRI dhan32 and BRRI dhan47. The highest frequency of callus induction was obtained for BRRI dhan32 (64.44%) in MS medium supplemented with 2, 4-D (2.5 mgL(-1)) and Kin (1.0 mgL(-1)). Different concentrations of NaCl (2.9, 5.9, 8.8 and 11.7 gL(-1)) were used and its effect was recorded on the basis of viability of calli (VC), relative growth rate (RGR), tolerance index (TI) and relative water content (RWC). It was observed that in all cases BRRI dhan47 showed highest performance on tolerance to VC (45.33%), RGR (1.03%), TI (0.20%) and RWC (10.23%) with 11.7 gL(-1) NaCl. Plant regeneration capability was recorded after partial air desiccation pretreatment to calli for 15, 30, 45 and 60 h. In this case BRRI dhan32 gave maximum number of regeneration (76.19%) when 4 weeks old calli were desiccated for 45 h. It was observed that air desiccation was 2-3 folds more effective for enhancing green plantlet regeneration compared to controls. Furthermore, desiccated calli also showed the better capability to survive in NaCl induced abiotic stress; and gave 1.9 fold (88.80%) increased regeneration in 11.7 gL(-1) salt level for BRRI dhan47. Analysis of variance (ANOVA) showed that the genotypes, air desiccation and NaCl had significant effect on plant regeneration at P < 0.01.
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Claims 12
Claim · model-written summary
BRRI dhan32 had the highest callus induction frequency of 64.44% in supplemented MS medium.
- Taxon
- rice
Oryza sativat0 - Material
- BRRI dhan32e0
- Treatment
- MS medium supplemented with 2, 4-D (2.5 mgL(-1)) and Kin (1.0 mgL(-1))e0
- Outcome
- frequency of callus inductione0
- Direction
- higheste0
- Value
- 64.44%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest frequency of callus induction was obtained for BRRI dhan32 (64.44%) in MS medium supplemented with 2, 4-D (2.5 mgL(-1)) and Kin (1.0 mgL(-1)).”
t0“rice”
Claim · model-written summary
BRRI dhan47 had the highest callus viability under 11.7 g/L NaCl at 45.33%.
- Taxon
- riceOryza sativat0
- Material
- BRRI dhan47e0
- Treatment
- 11.7 gL(-1) NaCle0
- Outcome
- VCe0
- Direction
- higheste0
- Value
- 45.33%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BRRI dhan47 showed highest performance on tolerance to VC (45.33%), RGR (1.03%), TI (0.20%) and RWC (10.23%) with 11.7 gL(-1) NaCl.”
t0“rice”
Claim · model-written summary
BRRI dhan47 had the highest relative growth rate under 11.7 g/L NaCl at 1.03%.
- Taxon
- riceOryza sativat0
- Material
- BRRI dhan47e0
- Treatment
- 11.7 gL(-1) NaCle0
- Outcome
- RGRe0
- Direction
- higheste0
- Value
- 1.03%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BRRI dhan47 showed highest performance on tolerance to VC (45.33%), RGR (1.03%), TI (0.20%) and RWC (10.23%) with 11.7 gL(-1) NaCl.”
t0“rice”
Claim · model-written summary
BRRI dhan47 had the highest tolerance index under 11.7 g/L NaCl at 0.20%.
- Taxon
- riceOryza sativat0
- Material
- BRRI dhan47e0
- Treatment
- 11.7 gL(-1) NaCle0
- Outcome
- TIe0
- Direction
- higheste0
- Value
- 0.20%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BRRI dhan47 showed highest performance on tolerance to VC (45.33%), RGR (1.03%), TI (0.20%) and RWC (10.23%) with 11.7 gL(-1) NaCl.”
t0“rice”
Claim · model-written summary
BRRI dhan47 had the highest relative water content under 11.7 g/L NaCl at 10.23%.
- Taxon
- riceOryza sativat0
- Material
- BRRI dhan47e0
- Treatment
- 11.7 gL(-1) NaCle0
- Outcome
- RWCe0
- Direction
- higheste0
- Value
- 10.23%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BRRI dhan47 showed highest performance on tolerance to VC (45.33%), RGR (1.03%), TI (0.20%) and RWC (10.23%) with 11.7 gL(-1) NaCl.”
t0“rice”
Claim · model-written summary
BRRI dhan32 reached 76.19% regeneration after 45 hours of desiccation of four-week-old calli.
- Taxon
- riceOryza sativat0
- Material
- BRRI dhan32e0
- Treatment
- desiccated for 45 he0
- Outcome
- regeneratione0
- Direction
- maximume0
- Value
- 76.19%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BRRI dhan32 gave maximum number of regeneration (76.19%) when 4 weeks old calli were desiccated for 45 h.”
t0“rice”
Claim · model-written summary
Air desiccation increased green plantlet regeneration two- to three-fold versus controls.
- Taxon
- riceOryza sativat0
- Treatment
- air desiccatione0
- Outcome
- green plantlet regeneratione0
- Direction
- enhancinge0
- Value
- 2-3 foldse0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“air desiccation was 2-3 folds more effective for enhancing green plantlet regeneration compared to controls.”
t0“rice”
Claim · model-written summary
Desiccated rice calli survived NaCl stress better.
- Taxon
- riceOryza sativat0
- Treatment
- desiccatede0
- Outcome
- capability to survive in NaCl induced abiotic stresse0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“desiccated calli also showed the better capability to survive in NaCl induced abiotic stress;”
t0“rice”
Claim · model-written summary
At 11.7 g/L salt, desiccation increased BRRI dhan47 regeneration 1.9 fold to 88.80%.
- Taxon
- riceOryza sativat0
- Material
- BRRI dhan47e0
- Treatment
- 11.7 gL(-1) salt levele0
- Outcome
- regeneratione0
- Direction
- increasede0
- Value
- 1.9 fold (88.80%)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gave 1.9 fold (88.80%) increased regeneration in 11.7 gL(-1) salt level for BRRI dhan47.”
t0“rice”
Claim · model-written summary
Rice genotype significantly affected regeneration.
- Taxon
- riceOryza sativat0
- Material
- genotypese0
- Outcome
- plant regeneratione0
- Direction
- effecte0
- Value
- P < 0.01e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of variance (ANOVA) showed that the genotypes, air desiccation and NaCl had significant effect on plant regeneration at P < 0.01.”
t0“rice”
Claim · model-written summary
Air desiccation significantly affected rice plant regeneration.
- Taxon
- riceOryza sativat0
- Treatment
- air desiccatione0
- Outcome
- plant regeneratione0
- Direction
- effecte0
- Value
- P < 0.01e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of variance (ANOVA) showed that the genotypes, air desiccation and NaCl had significant effect on plant regeneration at P < 0.01.”
t0“rice”
Claim · model-written summary
NaCl significantly affected rice plant regeneration.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of variance (ANOVA) showed that the genotypes, air desiccation and NaCl had significant effect on plant regeneration at P < 0.01.”
t0“rice”
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See all 296 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.