Abstract
Current study determined the effects of different cultivation media and some factors on in vitro callus induction and shoot regeneration of Pakaumpuel rice (Oryza sativa L.), the Thai rice landrace. Pakaumpuel’s dehusked seeds were surface sterilized by 20% Sodium hypoclorite for 20 mins before washed and cultured on Murashige and Skoog (MS) medium in various concentrations of BAP (0, 0.1, 0.5 and 1 mg/l) and 2,4-D (0, 1, 2 and 3 mg/l) and cultivated in off -light or dark cycle condition for 15 days. Results showed that MS medium with 2 mg/l 2,4-D incubated in light condition provided the highest callus induction percentage (80 %). For shoot regeneration, calli were desiccated by using silica gel for 0, 30, 90 and 180 minutes prior to culture on MS medium with 1 mg/l NAA and various concentrations of BAP (0, 1, 2, 3 and 4 mg/l) for 30 days. The results revealed that desiccation periods had no effect on regeneration percentage and number of shoot per callus, but had effects on shoot length and number of leaves per shoot. Therefore, MS medium with 1 mg/l NAA and 3 mg/l BAP induced the highest regeneration percentage (53.33 %) and number of shoot per callus (4.40 shoots), while the longest shoot (12.82 cm) and the highest number of leaves per shoot (3.00 leaves) were found on the culture on this medium after desiccation for 90 minutes. This discovery can be applied for micropropagation of other Thai rice landraces.
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Claims 7
Claim · model-written summary
MS medium with 2 mg/l 2,4-D in light gave the highest callus induction percentage (80 %).
- Taxon
- Oryza sativa L.
Oryza sativae0 - Material
- Pakaumpuele0
- Treatment
- MS medium with 2 mg/l 2,4-D incubated in light conditione1
- Outcome
- callus induction percentagee1
- Direction
- higheste1
- Value
- 80 %e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“effects of different cultivation media and some factors on in vitro callus induction and shoot regeneration of Pakaumpuel rice (Oryza sativa L.)”
e1“MS medium with 2 mg/l 2,4-D incubated in light condition provided the highest callus induction percentage (80 %)”
Claim · model-written summary
MS medium with 1 mg/l NAA and 3 mg/l BAP gave the highest shoot regeneration percentage (53.33 %).
- Taxon
- Oryza sativa L.Oryza sativae0
- Material
- Pakaumpuele0
- Treatment
- MS medium with 1 mg/l NAA and 3 mg/l BAPe2
- Outcome
- regeneration percentagee2
- Direction
- higheste2
- Value
- 53.33 %e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“effects of different cultivation media and some factors on in vitro callus induction and shoot regeneration of Pakaumpuel rice (Oryza sativa L.)”
e2“MS medium with 1 mg/l NAA and 3 mg/l BAP induced the highest regeneration percentage (53.33 %) and number of shoot per callus (4.40 shoots)”
Claim · model-written summary
MS medium with 1 mg/l NAA and 3 mg/l BAP gave the highest number of shoots per callus (4.40).
- Taxon
- Oryza sativa L.Oryza sativae0
- Material
- Pakaumpuele0
- Treatment
- MS medium with 1 mg/l NAA and 3 mg/l BAPe2
- Outcome
- number of shoot per calluse2
- Direction
- higheste2
- Value
- 4.40 shootse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“effects of different cultivation media and some factors on in vitro callus induction and shoot regeneration of Pakaumpuel rice (Oryza sativa L.)”
e2“MS medium with 1 mg/l NAA and 3 mg/l BAP induced the highest regeneration percentage (53.33 %) and number of shoot per callus (4.40 shoots)”
Claim · model-written summary
Callus desiccation periods had no effect on regeneration percentage or number of shoots per callus.
- Taxon
- Oryza sativa L.Oryza sativae0
- Material
- Pakaumpuele0
- Treatment
- desiccation periodse3
- Outcome
- regeneration percentage and number of shoot per calluse3
- Direction
- no effecte3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“effects of different cultivation media and some factors on in vitro callus induction and shoot regeneration of Pakaumpuel rice (Oryza sativa L.)”
e3“desiccation periods had no effect on regeneration percentage and number of shoot per callus”
Claim · model-written summary
Callus desiccated for 90 minutes gave the longest shoots (12.82 cm).
- Taxon
- Oryza sativa L.Oryza sativae0
- Material
- Pakaumpuele0
- Treatment
- desiccation for 90 minutese4
- Outcome
- longest shoote4
- Direction
- longeste4
- Value
- 12.82 cme4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“effects of different cultivation media and some factors on in vitro callus induction and shoot regeneration of Pakaumpuel rice (Oryza sativa L.)”
e4“the longest shoot (12.82 cm) and the highest number of leaves per shoot (3.00 leaves) were found on the culture on this medium after desiccation for 90 minutes”
Claim · model-written summary
Callus desiccated for 90 minutes gave the highest number of leaves per shoot (3.00).
- Taxon
- Oryza sativa L.Oryza sativae0
- Material
- Pakaumpuele0
- Treatment
- desiccation for 90 minutese4
- Outcome
- number of leaves per shoote4
- Direction
- higheste4
- Value
- 3.00 leavese4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“effects of different cultivation media and some factors on in vitro callus induction and shoot regeneration of Pakaumpuel rice (Oryza sativa L.)”
e4“the longest shoot (12.82 cm) and the highest number of leaves per shoot (3.00 leaves) were found on the culture on this medium after desiccation for 90 minutes”
Claim · model-written summary
The shoot regeneration method can be applied to micropropagation of other Thai rice landraces.
Verbatim
e5“This discovery can be applied for micropropagation of other Thai rice landraces”
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