Abstract
Partial resistance to the biotrophic fungal pathogen Claviceps purpurea, causal agent of ergot, has been found that colocates with mutant alleles of the wheat Reduced height (Rht) loci on chromosomes 4B and 4D. These Rht loci represent the wheat orthologs of the Arabidopsis Della genes. To investigate the role of the Rht mutant DELLA proteins in ergot resistance, we assessed C. purpurea infection in wheat near-isogenic lines (NILs) carrying the gibberellic acid (GA)-insensitive semidwarf alleles Rht-B1b and Rht-D1b and the severe dwarf alleles Rht-B1c and Rht-D1c. NILs of the GA-sensitive alleles Rht8 (chromosome 2D) and Rht12 (chromosome 5A) were also included. A general trend toward increased resistance to C. purpurea, with smaller and lighter sclerotia, was observed on the NILs Rht-B1b, Rht-D1b, Rht-B1c, and Rht-D1c, and also on Rht8. Levels of the bioactive GA 4 and the auxin indole-3-acetic acid increased after inoculation with C. purpurea, following similar patterns and implicating a potential auxin-mediated induction of GA biosynthesis. In contrast, jasmonic acid (JA) levels fell in the parental lines ‘Mercia’ and ‘Maris Huntsman’ after inoculation with C. purpurea, but increased in all the Rht-mutant NILs. Inoculation with C. purpurea did not show any informative changes in the levels of salicylic acid. Our results suggest that GA-mediated degradation of the DELLA proteins and down-regulation of JA-signaling pathways supports infection of wheat by C. purpurea. As these responses are generally associated with necrotrophic fungal pathogens, we propose that the biotroph C. purpurea may have a necrotrophic growth stage.
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Claims 10
Claim · model-written summary
Several Rht mutant lines and Rht8 tended toward increased ergot resistance with smaller, lighter sclerotia.
- Taxon
- Wheat
Triticum aestivumt0 - Material
- NILs Rht-B1b, Rht-D1b, Rht-B1c, and Rht-D1c, and also on Rht8e0
- Interactor
- C. purpurea
Claviceps purpureae0pathogen - Outcome
- resistance to C. purpureae0
- Direction
- increasede0
- Qualifier
- smaller and lighter sclerotiae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“A general trend toward increased resistance to C. purpurea, with smaller and lighter sclerotia, was observed on the NILs Rht-B1b, Rht-D1b, Rht-B1c, and Rht-D1c, and also on Rht8.”
Claim · model-written summary
C. purpurea sclerotia were smaller on these mutant lines.
- Taxon
- WheatTriticum aestivumt0
- Material
- NILs Rht-B1b, Rht-D1b, Rht-B1c, and Rht-D1c, and also on Rht8e0
- Interactor
- C. purpureaClaviceps purpureae0pathogen
- Outcome
- sclerotiae0
- Direction
- smallere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“increased resistance to C. purpurea, with smaller and lighter sclerotia, was observed on the NILs Rht-B1b, Rht-D1b, Rht-B1c, and Rht-D1c, and also on Rht8.”
Claim · model-written summary
C. purpurea sclerotia were lighter on these mutant lines.
- Taxon
- WheatTriticum aestivumt0
- Material
- NILs Rht-B1b, Rht-D1b, Rht-B1c, and Rht-D1c, and also on Rht8e0
- Interactor
- C. purpureaClaviceps purpureae0pathogen
- Outcome
- sclerotiae0
- Direction
- lightere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“increased resistance to C. purpurea, with smaller and lighter sclerotia, was observed on the NILs Rht-B1b, Rht-D1b, Rht-B1c, and Rht-D1c, and also on Rht8.”
Claim · model-written summary
GA 4 increased after C. purpurea inoculation.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- inoculation with C. purpureae0
- Interactor
- C. purpureaClaviceps purpureae0pathogen
- Outcome
- GA 4e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“Levels of the bioactive GA 4 and the auxin indole-3-acetic acid increased after inoculation with C. purpurea”
Claim · model-written summary
indole-3-acetic acid increased after C. purpurea inoculation.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- inoculation with C. purpureae0
- Interactor
- C. purpureaClaviceps purpureae0pathogen
- Outcome
- indole-3-acetic acide0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“Levels of the bioactive GA 4 and the auxin indole-3-acetic acid increased after inoculation with C. purpurea”
Claim · model-written summary
Jasmonic acid fell after inoculation in parental lines.
- Taxon
- WheatTriticum aestivumt0
- Material
- ‘Mercia’ and ‘Maris Huntsman’e0
- Treatment
- inoculation with C. purpureae0
- Interactor
- C. purpureaClaviceps purpureae0pathogen
- Outcome
- jasmonic acid (JA) levelse0
- Direction
- felle0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“jasmonic acid (JA) levels fell in the parental lines ‘Mercia’ and ‘Maris Huntsman’ after inoculation with C. purpurea”
Claim · model-written summary
Jasmonic acid increased after inoculation in all Rht mutant lines.
- Taxon
- WheatTriticum aestivumt0
- Material
- Rht-mutant NILse0
- Treatment
- inoculation with C. purpureae0
- Interactor
- C. purpureaClaviceps purpureae0pathogen
- Outcome
- jasmonic acid (JA) levelse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“jasmonic acid (JA) levels fell in the parental lines ‘Mercia’ and ‘Maris Huntsman’ after inoculation with C. purpurea, but increased in all the Rht-mutant NILs”
Claim · model-written summary
Inoculation showed no informative salicylic acid change.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- Inoculation with C. purpureae0
- Interactor
- C. purpureaClaviceps purpureae0pathogen
- Outcome
- levels of salicylic acide0
- Direction
- did not show any informative changese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“Inoculation with C. purpurea did not show any informative changes in the levels of salicylic acid.”
Claim · model-written summary
Authors suggest GA-mediated DELLA degradation and JA pathway down-regulation support infection.
- Taxon
- wheatTriticum aestivume0
- Interactor
- C. purpureaClaviceps purpureae0pathogen
- Gene
- DELLA proteinse0
- Outcome
- infection of wheat by C. purpureae0
- Qualifier
- down-regulation of JA-signaling pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“GA-mediated degradation of the DELLA proteins and down-regulation of JA-signaling pathways supports infection of wheat by C. purpurea”
Claim · model-written summary
Authors propose a necrotrophic growth stage for C. purpurea.
- Taxon
- WheatTriticum aestivumt0
- Interactor
- C. purpureaClaviceps purpureae0pathogen
- Outcome
- necrotrophic growth stagee0
- Qualifier
- may havee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e0“we propose that the biotroph C. purpurea may have a necrotrophic growth stage”
The same questions in other papers
inoculation · physiology: hormones · Triticum aestivum (wheat)Positive and negative results
16 in the opposite direction
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- positive
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See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.