Abstract
The grain filling of inferior spikelets is much less complete than that of superior spikelets in rice cultivars with large panicles and numerous spikelets and is promoted by moderate soil drying (MD) post-anthesis. A growing body of evidence has shown that microRNAs function in regulating grain development. However, little is known about the mechanism of microRNA control of grain filling of inferior spikelets in response to MD. In this study, grain filling of inferior spikelets was promoted by MD treatment in Nipponbare. Small-RNA profiling at the most active grain-filling stage was conducted in inferior spikelets under control (CK) and MD treatment. Of 521 known and 128 novel miRNAs, 38 known and 9 novel miRNAs were differentially expressed between the CK and MD treatments. Target genes of differentially expressed miRNAs were involved in multiple developmental and signaling pathways associated with catalytic activity, carbohydrate metabolism, and other functions. Both miR1861 and miR397 were upregulated by MD, leading to a decrease in OsSBDCP1 and OsLAC, two negative regulators of SSIIIa activity and BR signaling, respectively. In contrast, miR1432 abundance was reduced by MD, resulting in upregulation of OsACOT and thus an elevated content of both ABA and IAA. These results suggest that both starch synthesis and phytohormone biosynthesis are regulated by differentially expressed miRNAs in inferior spikelets in response to MD treatment. Our results suggest the molecular mechanisms by which miRNAs regulate grain filling in inferior spikelets of rice under moderate soil drying, providing potential application in agriculture to increase rice yields by genetic approaches.
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Claims 12
Claim · model-written summary
Moderate soil drying promoted grain filling of inferior spikelets in Nipponbare.
- Taxon
- rice
Oryza sativat0 - Material
- Nipponbaree0
- Organ / stage
- inferior spikeletse0
- Treatment
- MD treatmente0
- Outcome
- grain fillinge0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“grain filling of inferior spikelets was promoted by MD treatment in Nipponbare”
t0“rice inferior spikelets”
Claim · model-written summary
Of the identified microRNAs, 38 known microRNAs differed between control and soil drying.
- Taxon
- riceOryza sativat0
- Outcome
- miRNAse0
- Value
- 38 knowne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Of 521 known and 128 novel miRNAs, 38 known and 9 novel miRNAs were differentially expressed between the CK and MD treatments”
t0“rice inferior spikelets”
Claim · model-written summary
Nine novel microRNAs differed between control and soil drying.
- Taxon
- riceOryza sativat0
- Outcome
- novel miRNAse0
- Value
- 9 novele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“9 novel miRNAs were differentially expressed between the CK and MD treatments”
t0“rice inferior spikelets”
Claim · model-written summary
Target genes of the differentially expressed microRNAs were involved in developmental and signaling pathways.
- Taxon
- riceOryza sativat0
- Outcome
- involved in multiple developmental and signaling pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Target genes of differentially expressed miRNAs were involved in multiple developmental and signaling pathways associated with catalytic activity, carbohydrate metabolism, and other functions”
t0“rice inferior spikelets”
Claim · model-written summary
Moderate drying upregulated miR1861.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both miR1861 and miR397 were upregulated by MD”
t0“rice inferior spikelets”
Claim · model-written summary
Moderate drying upregulated miR397.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both miR1861 and miR397 were upregulated by MD”
t0“rice inferior spikelets”
Claim · model-written summary
Moderate drying decreased OsSBDCP1 downstream of microRNA upregulation.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both miR1861 and miR397 were upregulated by MD, leading to a decrease in OsSBDCP1 and OsLAC”
t0“rice inferior spikelets”
Claim · model-written summary
Moderate drying decreased OsLAC downstream of microRNA upregulation.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both miR1861 and miR397 were upregulated by MD, leading to a decrease in OsSBDCP1 and OsLAC”
t0“rice inferior spikelets”
Claim · model-written summary
Moderate drying reduced miR1432 abundance.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR1432 abundance was reduced by MD”
t0“rice inferior spikelets”
Claim · model-written summary
Moderate drying upregulated OsACOT following lower miR1432 abundance.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR1432 abundance was reduced by MD, resulting in upregulation of OsACOT”
t0“rice inferior spikelets”
Claim · model-written summary
Moderate drying elevated ABA content.
- Taxon
- riceOryza sativat0
- Treatment
- MDe1
- Gene
- OsACOTe1
- Outcome
- elevated content of both ABA and IAAe0
- Direction
- elevatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“thus an elevated content of both ABA and IAA”
e1“miR1432 abundance was reduced by MD, resulting in upregulation of OsACOT”
t0“rice inferior spikelets”
Claim · model-written summary
The authors suggest differentially expressed microRNAs regulate starch synthesis and phytohormone biosynthesis in drying treated inferior spikelets.
- Taxon
- riceOryza sativat0
- Organ / stage
- inferior spikeletse0
- Treatment
- MD treatmente0
- Outcome
- starch synthesis and phytohormone biosynthesise0
- Qualifier
- regulated by differentially expressed miRNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both starch synthesis and phytohormone biosynthesis are regulated by differentially expressed miRNAs in inferior spikelets in response to MD treatment”
t0“rice inferior spikelets”
The same questions in other papers
processing · yield · Oryza sativa (rice)Positive and negative results
87 papers in the same direction as this paper · 21 in the opposite direction · 5 reporting no difference from an alternative
- positive
- negative
- no effect
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