Abstract
Low temperature is a major environmental factor that severely impairs plant growth and productivity. Watermelon ( Citrullus lanatus ) is a chilling-sensitive crop. Grafting of watermelon onto pumpkin rootstock is an effective technique to increase the chilling tolerance of watermelon when exposure to short-time chilling stress. However, the mechanism by which pumpkin rootstock increases chilling tolerance remains poorly understood. Under 10°C/5°C (day/night) chilling stress treatment, pumpkin-grafted watermelon seedlings showed higher chilling tolerance than self-grafted watermelon plants with significantly reduced lipid peroxidation and chilling injury (CI) index. Physiological analysis revealed that pumpkin rootstock grafting led to the notable accumulation of putrescine in watermelon seedlings under chilling conditions. Pre-treat foliar with 1 mM D-arginine (inhibitor of arginine decarboxylase, ADC) increased the electrolyte leakage (EL) of pumpkin-grafted watermelon leaves under chilling stress. This result can be ascribed to the decrease in transcript levels of ADC , ornithine decarboxylase , spermidine synthase , and polyamine oxidase genes involved in the synthesis and metabolism of polyamines. Transcriptome analysis showed that pumpkin rootstock improved chilling tolerance in watermelon seedlings by regulating differential gene expression under chilling stress. Pumpkin-grafted seedling reduced the number and expression level of differential genes in watermelon scion under chilling stress. It specifically increased the up-regulated expression of ADC ( Cla97C11G210580 ), a key gene in the polyamine metabolism pathway, and ultimately promoted the accumulation of putrescine. In conclusion, pumpkin rootstock grafting increased the chilling tolerance of watermelon through transcription adjustments, up regulating the expression level of ADC , and promoting the synthesis of putrescine, which ultimately improved the chilling tolerance of pumpkin-grafted watermelon plants.
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Claims 11
Claim · model-written summary
Pumpkin-grafted watermelon had higher chilling tolerance than self-grafted plants under chilling stress.
- Taxon
- watermelon
Citrullus lanatuse0 - Treatment
- pumpkin-graftede0
- Outcome
- chilling tolerancee0
- Direction
- highere0
- Qualifier
- 10°C/5°C (day/night) chilling stress treatmente0
- Comparator
- self-grafted watermelon plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under 10°C/5°C (day/night) chilling stress treatment, pumpkin-grafted watermelon seedlings showed higher chilling tolerance than self-grafted watermelon plants with significantly reduced lipid peroxidation and chilling injury (CI) index.”
Claim · model-written summary
Pumpkin grafting reduced lipid peroxidation in watermelon under chilling stress.
- Taxon
- watermelonCitrullus lanatuse0
- Treatment
- pumpkin-graftede0
- Outcome
- lipid peroxidatione0
- Direction
- reducede0
- Comparator
- self-grafted watermelon plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pumpkin-grafted watermelon seedlings showed higher chilling tolerance than self-grafted watermelon plants with significantly reduced lipid peroxidation and chilling injury (CI) index”
Claim · model-written summary
Pumpkin grafting reduced the watermelon chilling injury index.
- Taxon
- watermelonCitrullus lanatuse0
- Treatment
- pumpkin-graftede0
- Outcome
- chilling injury (CI) indexe0
- Direction
- reducede0
- Comparator
- self-grafted watermelon plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pumpkin-grafted watermelon seedlings showed higher chilling tolerance than self-grafted watermelon plants with significantly reduced lipid peroxidation and chilling injury (CI) index”
Claim · model-written summary
Pumpkin rootstock grafting increased putrescine accumulation in chilled watermelon seedlings.
- Taxon
- watermelonCitrullus lanatuse0
- Treatment
- pumpkin rootstock graftinge0
- Outcome
- accumulation of putrescinee0
- Direction
- accumulatione0
- Qualifier
- under chilling conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pumpkin rootstock grafting led to the notable accumulation of putrescine in watermelon seedlings under chilling conditions”
Claim · model-written summary
D-arginine pretreatment increased electrolyte leakage in chilled pumpkin-grafted watermelon leaves.
- Taxon
- watermelonCitrullus lanatuse0
- Organ / stage
- leavese0
- Treatment
- 1 mM D-argininee0
- Outcome
- electrolyte leakage (EL)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pre-treat foliar with 1 mM D-arginine (inhibitor of arginine decarboxylase, ADC) increased the electrolyte leakage (EL) of pumpkin-grafted watermelon leaves under chilling stress”
Claim · model-written summary
D-arginine pretreatment decreased transcripts of polyamine synthesis and metabolism genes in chilled watermelon.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- D-argininee1
- Outcome
- transcript levels of ADC , ornithine decarboxylase , spermidine synthase , and polyamine oxidase genese0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This result can be ascribed to the decrease in transcript levels of ADC , ornithine decarboxylase , spermidine synthase , and polyamine oxidase genes involved in the synthesis and metabolism of polyamines.”
t0“Grafting Watermelon Onto Pumpkin Increases Chilling Tolerance by Up Regulating Arginine Decarboxylase to Increase Putrescine Biosynthesis”
e1“Pre-treat foliar with 1 mM D-arginine (inhibitor of arginine decarboxylase, ADC) increased the electrolyte leakage (EL) of pumpkin-grafted watermelon leaves under chilling stress”
Claim · model-written summary
Pumpkin rootstock regulated differential gene expression in chilled watermelon seedlings.
- Taxon
- watermelonCitrullus lanatuse0
- Treatment
- pumpkin rootstocke0
- Outcome
- chilling tolerancee0
- Qualifier
- regulating differential gene expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pumpkin rootstock improved chilling tolerance in watermelon seedlings by regulating differential gene expression under chilling stress”
Claim · model-written summary
Pumpkin grafting reduced the number of differential genes in chilled watermelon scions.
- Taxon
- watermelonCitrullus lanatuse0
- Organ / stage
- scione0
- Treatment
- Pumpkin-graftede0
- Outcome
- numbere0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pumpkin-grafted seedling reduced the number and expression level of differential genes in watermelon scion under chilling stress”
Claim · model-written summary
Pumpkin grafting reduced the expression level of differential genes in chilled watermelon scions.
- Taxon
- watermelonCitrullus lanatuse0
- Organ / stage
- scione0
- Treatment
- Pumpkin-graftede0
- Outcome
- expression level of differential genese0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pumpkin-grafted seedling reduced the number and expression level of differential genes in watermelon scion under chilling stress”
Claim · model-written summary
Pumpkin grafting increased up-regulated ADC expression in chilled watermelon.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- pumpkint0
- Gene
- ADC ( Cla97C11G210580 )e0
- Outcome
- expression of ADCe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It specifically increased the up-regulated expression of ADC ( Cla97C11G210580 ), a key gene in the polyamine metabolism pathway, and ultimately promoted the accumulation of putrescine.”
t0“Grafting Watermelon Onto Pumpkin Increases Chilling Tolerance by Up Regulating Arginine Decarboxylase to Increase Putrescine Biosynthesis”
Claim · model-written summary
Increased ADC expression promoted putrescine accumulation in pumpkin-grafted watermelon.
- Taxon
- watermelonCitrullus lanatust0
- Gene
- ADC ( Cla97C11G210580 )e0
- Outcome
- accumulation of putrescinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased the up-regulated expression of ADC ( Cla97C11G210580 ), a key gene in the polyamine metabolism pathway, and ultimately promoted the accumulation of putrescine”
t0“Grafting Watermelon Onto Pumpkin Increases Chilling Tolerance by Up Regulating Arginine Decarboxylase to Increase Putrescine Biosynthesis”
The same questions in other papers
grafting · molecular: metabolites · Citrullus lanatus (watermelon)Results differ
12 papers in the same direction as this paper · 6 in the opposite direction · 1 reporting no difference from an alternative
- no effect
- negative
- no effect
16 papers more in this group
See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.