Paper · 2022 · Journal of Tropical Life Science

Utilization of Various Organic Wastes as Liquid Biofertilizer Carrier Agents towards Viability of Bacteria and Green Bean Growth

Novi Arfarita; Tsuyoshi Imai; Cahyo Prayogo

5
claims
10
verbatim quotes
2
citations
2
research areas
✓
Open access · cc-by-nc

Abstract

The problem in the production of biofertilizers is that raw materials are cheap, easy to get and apply. Another problem is determining the viability of the consortium microbes in a biofertilizer formulation. This study aims to determine the bacterial viability of various liquid media originating from organic waste as a liquid biofertilizer carrier. Three indigenous bacterial strains under consortium for phosphate soluble (Pantoea ananatis strain 53 (BC32)), non-symbiostic Nitrogen fixation (Bacillus licheniformis strain S45) and stabilizing soil aggregate (Pseudomonas plecoglossidastrain PR19) were added to liquid biofertilizer. The study evaluated 10 treatments using a randomized design with three replicates. The treatments are as follows: Peptone,molasses, Compost wash from seaweed waste, Vermiwash, molasses + glycerol, compost wash from seaweed waste + glycerol, vermiwash + glycerol, molasses + PEG (PolyEthylene Glycols)1%, compost wash seaweed waste + PEG 1%, and Vermiwash + PEG 1%. This biofertilizer formulation (liquid) was kept for 16 weeks at optimumpH 5.5. VP3 (Vermiwash made from vermicompost + PEG 1%) treatment showed thebest viability of bacterial strains during the 16-week storage period. The pathogenicitytest using green bean seeds Vima-1 showed that all liquid formulations of biological fertilizers with the three consortium bacterial isolates did not show signs of diseases and demonstrated better growth than the control treatment. Compared to other treatments, the best growth of bacterial strains was detected with MP2 (Molasses + glycerol) treatment. Formulations using vermiwash and PEG appear to maintain bacterial viability in the formulation effectively. However, the formulation of molasses and glycerol exerts a stimulating effect on sprouts growth.

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Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The VP3 formulation (vermiwash from vermicompost with PEG 1%) showed the best viability of bacterial strains during 16 weeks of storage.

Taxon
green beant0
Treatment
Vermiwash made from vermicompost + PEG 1%e0
Interactor
bacterial strainse0symbiont
Outcome
viability of bacterial strainse0
Direction
beste0

Not reportedComparator

Verbatim

t0

“Green Bean Growth”

✓ located verbatim in the source · title, characters 111–128 · show in text ↑
e0

“VP3 (Vermiwash made from vermicompost + PEG 1%) treatment showed thebest viability of bacterial strains during the 16-week storage period”

✓ located verbatim in the source · abstract, characters 1082–1219 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The molasses and glycerol formulation gave the best growth of bacterial strains compared with other treatments.

Taxon
green beant0
Treatment
Molasses + glycerole1
Interactor
bacterial strainse1symbiont
Outcome
growth of bacterial strainse1
Direction
beste1
Comparator
other treatmentse1

Verbatim

t0

“Green Bean Growth”

✓ located verbatim in the source · title, characters 111–128 · show in text ↑
e1

“Compared to other treatments, the best growth of bacterial strains was detected with MP2 (Molasses + glycerol) treatment”

✓ located verbatim in the source · abstract, characters 1470–1590 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

All liquid formulations with the three consortium bacterial isolates gave better green bean growth than the control treatment.

Taxon
green beane2
Treatment
liquid formulations of biological fertilizers with the three consortium bacterial isolatese2
Interactor
three consortium bacterial isolatese2symbiont
Outcome
growthe2
Direction
bettere2
Comparator
control treatmente2

Verbatim

t0

“Green Bean Growth”

✓ located verbatim in the source · title, characters 111–128 · show in text ↑
e2

“The pathogenicity test using green bean seeds Vima-1 showed that all liquid formulations of biological fertilizers with the three consortium bacterial isolates did not show signs of diseases and demonstrated better growth than the control treatment”

✓ located after whitespace/quote normalisation · abstract, characters 1221–1468 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The molasses and glycerol formulation has a stimulating effect on sprout growth of green bean.

Taxon
green beant0
Treatment
the formulation of molasses and glycerole3
Outcome
sprouts growthe3
Direction
stimulating effecte3

Not reportedComparator

Verbatim

t0

“Green Bean Growth”

✓ located verbatim in the source · title, characters 111–128 · show in text ↑
e3

“the formulation of molasses and glycerol exerts a stimulating effect on sprouts growth”

✓ located verbatim in the source · abstract, characters 1709–1795 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

In the pathogenicity test, green bean seedlings treated with the liquid formulations did not show signs of disease.

Taxon
green beant0
Outcome
signs of diseasese4

Verbatim

t0

“Green Bean Growth”

✓ located verbatim in the source · title, characters 111–128 · show in text ↑
e4

“did not show signs of diseases”

✓ located verbatim in the source · abstract, characters 1380–1410 · show in text ↑

glycerol · growth · Phaseolus vulgaris (common bean)Positive and negative results

3 papers: 1 positive, 2 negative or no effect

2 in the opposite direction

  1. negative
  2. no effect
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molasses · growth · Phaseolus vulgaris (common bean)Results agree

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drought · growth · Phaseolus vulgaris (common bean)Results differ

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inoculation · growth · Phaseolus vulgaris (common bean)Results differ

126 papers: 106 positive, 20 negative or no effect

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fertilization · growth · Phaseolus vulgaris (common bean)Positive and negative results

143 papers: 121 positive, 22 negative or no effect

120 papers in the same direction as this paper · 22 in the opposite direction

  1. negative
  2. no effect
  3. no effect

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.