Abstract
BACKGROUND: Plant growth-promoting rhizobacteria (PGPR) release volatile organic compounds (VOCs), which promote plant growth. RESULTS: A potential PGPR strain GX14001 was isolated from marine samples, and the VOCs produced by GX14001 significantly promoted tobacco (Nicotiana benthamiana) growth in a plate experiment. Based on 16S rRNA sequence alignment and physiological and biochemical characterization, GX14001 was identified as Microbacterium aurantiacum. Comparative transcriptome analysis was conducted between GX14001 VOCs-treated tobacco and the control; it was found that 1286 genes were upregulated and 1088 genes were downregulated. Gene ontology (GO) analysis showed that upregulated genes were involved in three biological processes: polysaccharide metabolic, polysaccharide catabolic and carbohydrate metabolic. The downregulated genes were involved in six biological processes, namely cell redox homeostasis, cellular homeostasis, carbohydrate metabolic process, homeostatic process, obsolete electron transport, and regulation of biological quality. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis showed that 190 upregulated differentially expressed genes were mainly involved in plant hormone signal transduction, phenylpropyl biosynthesis, plant-pathogen interaction, and flavonoid biosynthesis. The 148 downregulated differentially expressed genes were mainly involved in plant hormone signal transduction and the metabolism of ascorbic, aldehyde, and pyruvate acids. Further analysis revealed that many genes were differentially expressed in the metabolic pathways of plant hormone signals, which were speculated to be the main reason why GX14001 VOCs promoted tobacco growth. To further study its regulatory mechanism, we found that GX14001 promoted plant growth through auxin, salicylic acid, and gibberellin in Arabidopsis mutant experiments. CONCLUSION: The VOCs produced by Microbacterium aurantiacum GX14001 may promote the growth of tobacco through the auxin, salicylic acid and gibberellin pathways.
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Claims 10
Claim · model-written summary
VOCs produced by strain GX14001 significantly promoted tobacco growth in a plate experiment.
- Taxon
- tobacco (Nicotiana benthamiana)
Nicotiana benthamianae0 - Treatment
- VOCs produced by GX14001e0
- Interactor
- GX14001e0other
- Outcome
- growthe0
- Direction
- promotede0
- Setting
- plate experimente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco (Nicotiana benthamiana)”
e0“the VOCs produced by GX14001 significantly promoted tobacco (Nicotiana benthamiana) growth in a plate experiment”
Claim · model-written summary
Strain GX14001 was identified as Microbacterium aurantiacum by 16S rRNA alignment and physiological and biochemical characterization.
- Taxon
- Microbacterium aurantiacumMicrobacterium aurantiacume0
- Outcome
- identified as Microbacterium aurantiacume0
Verbatim
e0“GX14001 was identified as Microbacterium aurantiacum”
Claim · model-written summary
In GX14001 VOCs-treated tobacco compared with the control, 1286 genes were upregulated.
- Taxon
- tobacco (Nicotiana benthamiana)Nicotiana benthamianat0
- Treatment
- GX14001 VOCs-treatede0
- Outcome
- genese0
- Direction
- upregulatede0
- Value
- 1286e0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco (Nicotiana benthamiana)”
e0“Comparative transcriptome analysis was conducted between GX14001 VOCs-treated tobacco and the control; it was found that 1286 genes were upregulated and 1088 genes were downregulated”
Claim · model-written summary
In GX14001 VOCs-treated tobacco compared with the control, 1088 genes were downregulated.
- Taxon
- tobacco (Nicotiana benthamiana)Nicotiana benthamianat0
- Treatment
- GX14001 VOCs-treatede0
- Outcome
- genese0
- Direction
- downregulatede0
- Value
- 1088e0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco (Nicotiana benthamiana)”
e0“Comparative transcriptome analysis was conducted between GX14001 VOCs-treated tobacco and the control; it was found that 1286 genes were upregulated and 1088 genes were downregulated”
Claim · model-written summary
GO analysis showed upregulated genes were involved in polysaccharide metabolic, polysaccharide catabolic and carbohydrate metabolic processes.
- Taxon
- tobacco (Nicotiana benthamiana)Nicotiana benthamianat0
- Outcome
- polysaccharide metabolic, polysaccharide catabolic and carbohydrate metabolice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco (Nicotiana benthamiana)”
e0“upregulated genes were involved in three biological processes: polysaccharide metabolic, polysaccharide catabolic and carbohydrate metabolic”
Claim · model-written summary
Downregulated genes were involved in six biological processes: cell redox homeostasis, cellular homeostasis, carbohydrate metabolic process, homeostatic process, obsolete electron transport and regulation of biological quality.
- Taxon
- tobacco (Nicotiana benthamiana)Nicotiana benthamianat0
- Outcome
- cell redox homeostasis, cellular homeostasis, carbohydrate metabolic process, homeostatic process, obsolete electron transport, and regulation of biological qualitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco (Nicotiana benthamiana)”
e0“The downregulated genes were involved in six biological processes, namely cell redox homeostasis, cellular homeostasis, carbohydrate metabolic process, homeostatic process, obsolete electron transport, and regulation of biological quality”
Claim · model-written summary
The 190 upregulated differentially expressed genes were mainly involved in plant hormone signal transduction, phenylpropyl biosynthesis, plant-pathogen interaction and flavonoid biosynthesis (KEGG).
- Taxon
- tobacco (Nicotiana benthamiana)Nicotiana benthamianat0
- Outcome
- plant hormone signal transduction, phenylpropyl biosynthesis, plant-pathogen interaction, and flavonoid biosynthesise0
- Value
- 190e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco (Nicotiana benthamiana)”
e0“190 upregulated differentially expressed genes were mainly involved in plant hormone signal transduction, phenylpropyl biosynthesis, plant-pathogen interaction, and flavonoid biosynthesis”
Claim · model-written summary
The 148 downregulated differentially expressed genes were mainly involved in plant hormone signal transduction and the metabolism of ascorbic, aldehyde and pyruvate acids (KEGG).
- Taxon
- tobacco (Nicotiana benthamiana)Nicotiana benthamianat0
- Outcome
- plant hormone signal transduction and the metabolism of ascorbic, aldehyde, and pyruvate acidse0
- Value
- 148e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco (Nicotiana benthamiana)”
e0“The 148 downregulated differentially expressed genes were mainly involved in plant hormone signal transduction and the metabolism of ascorbic, aldehyde, and pyruvate acids”
Claim · model-written summary
Differential expression of many genes in plant hormone signal pathways was speculated to be the main reason GX14001 VOCs promoted tobacco growth.
- Taxon
- tobacco (Nicotiana benthamiana)Nicotiana benthamianat0
- Treatment
- GX14001 VOCse0
- Outcome
- tobacco growthe0
- Qualifier
- speculatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco (Nicotiana benthamiana)”
e0“many genes were differentially expressed in the metabolic pathways of plant hormone signals, which were speculated to be the main reason why GX14001 VOCs promoted tobacco growth”
Claim · model-written summary
Arabidopsis mutant experiments indicated that GX14001 promoted plant growth through auxin, salicylic acid and gibberellin.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- GX14001e0
- Interactor
- GX14001e0other
- Compound
- auxin, salicylic acid, and gibberelline0
- Outcome
- plant growthe0
- Setting
- Arabidopsis mutant experimentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we found that GX14001 promoted plant growth through auxin, salicylic acid, and gibberellin in Arabidopsis mutant experiments”
The same questions in other papers
volatile organic compound · growth · Nicotiana benthamianaResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.