Abstract
Abstract A novel monopartite begomovirus was found in naturally infected Conyza canadensis plants exhibiting typical begomovirus-induced yellow vein symptoms in Yunnan Province of China. Analysis of two obtained full-length viral genome sequences showed that they shared 99.8% nucleotide (nt) sequence similarity, and both consisted of 2733 nts (GenBank accession no. OK120264 and OK120266, respectively). Further analysis showed that these two sequences shared the highest nt sequence similarity (78.9%) with tomato yellow leaf curl Vietnam virus (TYLCVV) (EU189150). In the assayed C. canadensis plants, a betasatellite of 1,341 nts (Accession no. OK120265 and OK120267, respectively) was also found, and it shared 98.1% sequence similarity with malvastrum yellow vein betasatellite (MaYVB) (JX679254). Based on the current classification criteria for begomoviruses, we consider that the two obtained viral isolates are a novel begomovirus, and named it as conyza yellow vein virus (CoYVV). Our further analysis result showed that CoYVV is likely originated from a recombination event between tomato yellow leaf curl Yunnan virus (TYLCYnV) and tomato yellow leaf curl China virus (TYLCCNV). To investigate the effect of CoYVV infection in plant, we constructed two infectious clones (i.e., pCoYVV and pMaYVB), and inoculated them individually or together to Nicotiana benthamiana plants through agro-infiltration. The result showed that the plants co-inoculated with CoYVV and MaYVB developed yellow vein and downward leaf-curling symptoms, whereas the plants inoculated with CoYVV alone showed no clear virus-like symptoms. Virus infection in the inoculated N. benthamiana plants was confirmed through polymerase chain reaction (PCR). The result of quantitative PCR (qPCR) showed that in the presence of MaYVB, the accumulation level of CoYVV DNA was significantly increased compared to that in the plants infected with CoYVV alone.
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Claims 11
Claim · model-written summary
Naturally infected Conyza canadensis had yellow vein symptoms.
- Taxon
- Conyza canadensis
Erigeron canadensise0 - Interactor
- begomovirus
Begomoviruse0pathogen - Outcome
- yellow vein symptomse0
- Setting
- Yunnan Province of Chinae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“naturally infected Conyza canadensis plants exhibiting typical begomovirus-induced yellow vein symptoms in Yunnan Province of China”
Claim · model-written summary
The two viral genomes shared 99.8% nucleotide sequence similarity.
- Taxon
- Conyza canadensisErigeron canadensist0
- Interactor
- begomovirusBegomovirust0pathogen
- Outcome
- nucleotide (nt) sequence similaritye0
- Value
- 99.8%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two obtained full-length viral genome sequences showed that they shared 99.8% nucleotide (nt) sequence similarity, and both consisted of 2733 nts”
t0“Conyza canadensis-infecting begomovirus”
Claim · model-written summary
The viral genomes had highest similarity of 78.9% to TYLCVV.
- Taxon
- Conyza canadensisErigeron canadensist0
- Interactor
- begomovirusBegomovirust0pathogen
- Outcome
- nt sequence similaritye0
- Value
- 78.9%e0
- Comparator
- tomato yellow leaf curl Vietnam virus (TYLCVV)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these two sequences shared the highest nt sequence similarity (78.9%) with tomato yellow leaf curl Vietnam virus (TYLCVV)”
t0“Conyza canadensis-infecting begomovirus”
Claim · model-written summary
A 1,341 nucleotide betasatellite was found in C. canadensis.
- Taxon
- C. canadensisErigeron canadensise0
- Outcome
- betasatellitee0
- Value
- 1,341 ntse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the assayed C. canadensis plants, a betasatellite of 1,341 nts”
Claim · model-written summary
The betasatellite shared 98.1% similarity with MaYVB.
- Taxon
- Conyza canadensisErigeron canadensist0
- Interactor
- begomovirusBegomovirust0pathogen
- Outcome
- sequence similaritye0
- Value
- 98.1%e0
- Comparator
- malvastrum yellow vein betasatellite (MaYVB)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it shared 98.1% sequence similarity with malvastrum yellow vein betasatellite (MaYVB)”
t0“Conyza canadensis-infecting begomovirus”
Claim · model-written summary
The isolates were named conyza yellow vein virus.
- Taxon
- Conyza canadensisErigeron canadensist0
- Interactor
- conyza yellow vein virus (CoYVV)Conyza yellow vein viruse0pathogen
- Outcome
- novel begomoviruse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the two obtained viral isolates are a novel begomovirus, and named it as conyza yellow vein virus (CoYVV)”
t0“Conyza canadensis-infecting begomovirus”
Claim · model-written summary
CoYVV likely originated through recombination between two tomato yellow leaf curl viruses.
- Taxon
- Conyza canadensisErigeron canadensist0
- Interactor
- CoYVVe0pathogen
- Outcome
- recombination evente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CoYVV is likely originated from a recombination event between tomato yellow leaf curl Yunnan virus (TYLCYnV) and tomato yellow leaf curl China virus (TYLCCNV)”
t0“Conyza canadensis-infecting begomovirus”
Claim · model-written summary
Co-inoculated Nicotiana benthamiana developed yellow vein and leaf curling symptoms.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Treatment
- co-inoculated with CoYVV and MaYVBe0
- Interactor
- CoYVVe0pathogen
- Second interactor
- MaYVBe0pathogen
- Outcome
- yellow vein and downward leaf-curling symptomse0
- Comparator
- inoculated with CoYVV alonee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plants co-inoculated with CoYVV and MaYVB developed yellow vein and downward leaf-curling symptoms, whereas the plants inoculated with CoYVV alone showed no clear virus-like symptoms”
e1“to Nicotiana benthamiana plants through agro-infiltration”
Claim · model-written summary
CoYVV alone produced no clear virus-like symptoms.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- inoculated with CoYVV alonee0
- Interactor
- CoYVVe0pathogen
- Outcome
- no clear virus-like symptomse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plants inoculated with CoYVV alone showed no clear virus-like symptoms”
e1“to Nicotiana benthamiana plants through agro-infiltration”
Claim · model-written summary
PCR confirmed virus infection in inoculated N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Interactor
- CoYVVe1pathogen
- Outcome
- Virus infectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Virus infection in the inoculated N. benthamiana plants was confirmed through polymerase chain reaction (PCR)”
e1“The result showed that the plants co-inoculated with CoYVV and MaYVB developed yellow vein”
Claim · model-written summary
MaYVB significantly increased CoYVV DNA accumulation relative to CoYVV alone.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- presence of MaYVBe0
- Interactor
- CoYVVe0pathogen
- Second interactor
- MaYVBe0pathogen
- Outcome
- accumulation level of CoYVV DNAe0
- Direction
- increasede0
- Comparator
- plants infected with CoYVV alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the presence of MaYVB, the accumulation level of CoYVV DNA was significantly increased compared to that in the plants infected with CoYVV alone”
e1“to Nicotiana benthamiana plants through agro-infiltration”
The same questions in other papers
inoculation · disease · Nicotiana benthamianaPositive and negative results
31 papers in the same direction as this paper · 8 in the opposite direction
- negative
- positive
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36 papers more in this group
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