Abstract
SUMMARY A technique to better ensure reliable nodulation of pot-grown, P. vulgaris plants with cultured strains of Rhizobium is described. Nodulation, dinitrogen fixation (acetylene reduction) activity and growth of 28-day-old plants were all stimulated markedly when seeds had been inoculated with cultured Rhizobium phaseoli suspended in alginate gel (Agrigel) rather than in sucrose solution. Nodulation attributes of plants inoculated with R. phaseoli strain RCR 3644 which had been sub-cultured repeatedly on yeast mannitol agar slopes were consistently inferior to those recorded for the same strain but recently cultured from a freeze-dried sample. Some of the potential problems with open pot culture of nodule-dependent legumes are discussed.
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Claims 4
Claim · model-written summary
Alginate gel inoculation increased nodulation relative to sucrose solution in 28-day-old P. vulgaris.
- Taxon
- Phaseolus vulgaris
Phaseolus vulgarist0 - Organ / stage
- 28-day-old plantse0
- Treatment
- inoculated with cultured Rhizobium phaseoli suspended in alginate gel (Agrigel)e0
- Interactor
- Rhizobium phaseoliRhizobium phaseolie0symbiont
- Outcome
- Nodulatione0
- Direction
- stimulated markedlye0
- Comparator
- in sucrose solutione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Phaseolus vulgaris”
e0“Nodulation, dinitrogen fixation (acetylene reduction) activity and growth of 28-day-old plants were all stimulated markedly when seeds had been inoculated with cultured Rhizobium phaseoli suspended in alginate gel (Agrigel) rather than in sucrose solution.”
Claim · model-written summary
Alginate gel inoculation increased dinitrogen fixation activity relative to sucrose solution.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Treatment
- inoculated with cultured Rhizobium phaseoli suspended in alginate gel (Agrigel)e0
- Interactor
- Rhizobium phaseoliRhizobium phaseolie0symbiont
- Outcome
- dinitrogen fixation (acetylene reduction) activitye0
- Direction
- stimulated markedlye0
- Comparator
- in sucrose solutione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Phaseolus vulgaris”
e0“Nodulation, dinitrogen fixation (acetylene reduction) activity and growth of 28-day-old plants were all stimulated markedly when seeds had been inoculated with cultured Rhizobium phaseoli suspended in alginate gel (Agrigel) rather than in sucrose solution.”
Claim · model-written summary
Alginate gel inoculation increased growth relative to sucrose solution.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Treatment
- inoculated with cultured Rhizobium phaseoli suspended in alginate gel (Agrigel)e0
- Interactor
- Rhizobium phaseoliRhizobium phaseolie0symbiont
- Outcome
- growthe0
- Direction
- stimulated markedlye0
- Comparator
- in sucrose solutione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Phaseolus vulgaris”
e0“Nodulation, dinitrogen fixation (acetylene reduction) activity and growth of 28-day-old plants were all stimulated markedly when seeds had been inoculated with cultured Rhizobium phaseoli suspended in alginate gel (Agrigel) rather than in sucrose solution.”
Claim · model-written summary
Repeatedly subcultured R. phaseoli RCR 3644 gave inferior nodulation attributes relative to the freeze-dried source.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Treatment
- R. phaseoli strain RCR 3644 which had been sub-cultured repeatedly on yeast mannitol agar slopese0
- Interactor
- R. phaseoli strain RCR 3644Rhizobium phaseolie0symbiont
- Outcome
- Nodulation attributese0
- Direction
- inferiore0
- Comparator
- the same strain but recently cultured from a freeze-dried samplee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Phaseolus vulgaris”
e0“Nodulation attributes of plants inoculated with R. phaseoli strain RCR 3644 which had been sub-cultured repeatedly on yeast mannitol agar slopes were consistently inferior to those recorded for the same strain but recently cultured from a freeze-dried sample.”
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