Paper · 1984 · Experimental Agriculture

An Assessment of Different Techniques for Inoculating Phaseolus vulgaris with Rhizobium

S. Rawsthorne; R. J. Summerfield

2
claims
4
verbatim quotes
7
citations
2
research areas
—
Not open access

Abstract

SUMMARY A technique to better ensure reliable nodulation of pot-grown, P. vulgaris plants with cultured strains of Rhizobium is described. Nodulation, dinitrogen fixation (acetylene reduction) activity and growth of 28-day-old plants were all stimulated markedly when seeds had been inoculated with cultured Rhizobium phaseoli suspended in alginate gel (Agrigel) rather than in sucrose solution. Nodulation attributes of plants inoculated with R. phaseoli strain RCR 3644 which had been sub-cultured repeatedly on yeast mannitol agar slopes were consistently inferior to those recorded for the same strain but recently cultured from a freeze-dried sample. Some of the potential problems with open pot culture of nodule-dependent legumes are discussed.

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Claims 2

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculating seeds with Rhizobium phaseoli in alginate gel markedly stimulated nodulation, dinitrogen fixation and growth of 28-day-old plants compared with sucrose solution.

Taxon
P. vulgarisPhaseolus vulgarise0
Organ / stage
28-day-old plantse1
Treatment
inoculated with cultured Rhizobium phaseoli suspended in alginate gel (Agrigel)e1
Interactor
Rhizobium phaseoliRhizobium phaseolie1symbiont
Outcome
Nodulation, dinitrogen fixation (acetylene reduction) activity and growthe1
Direction
stimulatede1
Comparator
sucrose solutione1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“reliable nodulation of pot-grown, P. vulgaris plants with cultured strains of Rhizobium is described”

✓ located verbatim in the source · abstract, characters 37–137 · show in text ↑
e1

“Nodulation, dinitrogen fixation (acetylene reduction) activity and growth of 28-day-old plants were all stimulated markedly when seeds had been inoculated with cultured Rhizobium phaseoli suspended in alginate gel (Agrigel) rather than in sucrose solution”

✓ located verbatim in the source · abstract, characters 139–394 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Strain RCR 3644 sub-cultured repeatedly on yeast mannitol agar gave nodulation attributes consistently inferior to the same strain recently cultured from freeze-dried samples.

Taxon
P. vulgarisPhaseolus vulgarise0
Material
strain RCR 3644e2
Treatment
sub-cultured repeatedly on yeast mannitol agar slopese2
Interactor
R. phaseoliRhizobium phaseolie2symbiont
Outcome
Nodulation attributese2
Direction
inferiore2
Comparator
recently cultured from a freeze-dried samplee2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“reliable nodulation of pot-grown, P. vulgaris plants with cultured strains of Rhizobium is described”

✓ located verbatim in the source · abstract, characters 37–137 · show in text ↑
e2

“Nodulation attributes of plants inoculated with R. phaseoli strain RCR 3644 which had been sub-cultured repeatedly on yeast mannitol agar slopes were consistently inferior to those recorded for the same strain but recently cultured from a freeze-dried sample”

✓ located verbatim in the source · abstract, characters 396–654 · show in text ↑

alginate · symbiosis · Phaseolus vulgaris (common bean)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Rhizobium phaseoli · symbiosis · Phaseolus vulgaris (common bean)Results agree

7 papers: 7 positive, 0 negative or no effect

6 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

3 papers more in this group

See all 7 papers →

inoculation · symbiosis · Phaseolus vulgaris (common bean)Positive and negative results

90 papers: 83 positive, 7 negative or no effect

82 papers in the same direction as this paper · 7 in the opposite direction

  1. no effect
  2. no effect
  3. negative

86 papers more in this group

See all 90 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.