Abstract
In vitro pollen germination was examined as a method to determine the mutagenic and physiological effects of environmental pollutants on higher organisms. Results were presented indicating that mutations could be distinguished by their in vitro germination characteristics if sporophytes homozygous for the mutated allele were tested. The addition of agents directly into the in vitro medium was shown to be an effective method to assess their physiological effects. The exposure of pollen grains during the in vitro germination process to ultraviolet radiation in the B range (280-320 nm) was found to produce little or no change in the germination or ruptured percentage but a sharp decrease in pollen tube growth after 1 hr. In vitro pollen germination appears to be a valuable method to examine the mutation types and physiological effects produced by a broad range of environmental pollutants. In fact, since in vitro germination is related to reproduction and gene transmission, the biological activity of these agents on in vitro germination should be tested routinely to determine their possible effects on food production and gene frequency changes in future generations.
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Claims 5
Claim · model-written summary
Homozygous mutations could be distinguished through in vitro pollen germination characteristics.
- Organ / stage
- Pollent0
- Outcome
- mutations could be distinguishede0
- Qualifier
- if sporophytes homozygous for the mutated allele were testede0
Zea maysNot reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutations could be distinguished by their in vitro germination characteristics if sporophytes homozygous for the mutated allele were tested”
t0“Maize Pollen”
Claim · model-written summary
Adding agents to the in vitro medium effectively assessed physiological effects.
- Treatment
- addition of agents directly into the in vitro mediume0
- Outcome
- assess their physiological effectse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of agents directly into the in vitro medium was shown to be an effective method to assess their physiological effects.”
t0“Maize”
Claim · model-written summary
UV-B exposure caused little or no change in pollen germination or rupture percentage.
- Organ / stage
- pollen grainse0
- Treatment
- ultraviolet radiation in the B range (280-320 nm)e0
- Outcome
- germination or ruptured percentagee0
- Direction
- little or no changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The exposure of pollen grains during the in vitro germination process to ultraviolet radiation in the B range (280-320 nm) was found to produce little or no change in the germination or ruptured percentage”
t0“Maize”
Claim · model-written summary
UV-B exposure sharply decreased pollen tube growth after one hour.
- Treatment
- ultraviolet radiation in the B range (280-320 nm)e0
- Outcome
- pollen tube growthe0
- Direction
- decreasee0
- Qualifier
- after 1 hre0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The exposure of pollen grains during the in vitro germination process to ultraviolet radiation in the B range (280-320 nm) was found to produce little or no change in the germination or ruptured percentage but a sharp decrease in pollen tube growth after 1 hr.”
t0“Maize”
Claim · model-written summary
The authors recommend routine pollen germination testing to assess pollutant effects on food production and gene frequencies.
- Outcome
- should be tested routinelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the biological activity of these agents on in vitro germination should be tested routinely to determine their possible effects on food production and gene frequency changes in future generations”
t0“Maize”
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