Abstract
Recalcitrant regeneration is one of the bottlenecks for plant improvement, especially in monocotyledonous species. In this research, plantlet regeneration of a native rice cultivar Oryza sativa ‘Barak Cenana’, high-value local Balinese red rice was successfully demonstrated. Compact nodular calli were induced from excised seed-derived mature embryos of ‘Barak Cenana’ rice. Although 2,4-D was necessary for callus induction, a combination of 2,4-D in regeneration medium caused reduction of green spot and shoot meristemoid differentiation. Root differentiation was firstly regenerated where meristematic shoots were thereafter developed from calli. The highest number of shoot regeneration regenerated from green spots and numbers of plantlet with complete shoot/root development were observed when transferred calli to Murashige and Skoog (MS) medium containing 5 mgL-1. Multiple shoot formation of regenerated plantlets revealed for efficient regeneration system, which was firstly reported in this present study and will potentially serve for plant improvement of this native rice, Barak Cenana.Â
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Claims 6
Claim · model-written summary
Compact nodular calli were induced from excised seed-derived mature embryos of Barak Cenana rice.
- Taxon
- Oryza sativa
Oryza sativat0 - Material
- Barak Cenanat0
- Organ / stage
- seed-derived mature embryose1
- Outcome
- Compact nodular callie1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Balinese red rice (Oryza sativa Cv. Barak Cenana)”
e1“Compact nodular calli were induced from excised seed-derived mature embryos”
Claim · model-written summary
A combination of 2,4-D in regeneration medium reduced green spot and shoot meristemoid differentiation in rice calli.
- Taxon
- Oryza sativaOryza sativat0
- Treatment
- 2,4-D in regeneration mediume2
- Outcome
- green spot and shoot meristemoid differentiatione2
- Direction
- reductione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Balinese red rice (Oryza sativa Cv. Barak Cenana)”
e2“a combination of 2,4-D in regeneration medium caused reduction of green spot and shoot meristemoid differentiation”
Claim · model-written summary
2,4-D was necessary for callus induction from mature embryos of rice.
- Taxon
- Oryza sativaOryza sativat0
- Treatment
- 2,4-De3
- Outcome
- callus inductione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Balinese red rice (Oryza sativa Cv. Barak Cenana)”
e3“Although 2,4-D was necessary for callus induction”
Claim · model-written summary
Root differentiation was regenerated first, and meristematic shoots developed from calli afterwards.
- Taxon
- Oryza sativaOryza sativat0
- Organ / stage
- callie4
- Outcome
- Root differentiatione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Balinese red rice (Oryza sativa Cv. Barak Cenana)”
e4“Root differentiation was firstly regenerated where meristematic shoots were thereafter developed from calli”
Claim · model-written summary
Shoot regeneration from green spots and plantlets with complete shoot/root development were highest on MS medium containing 5 mgL-1.
- Taxon
- Oryza sativaOryza sativat0
- Treatment
- Murashige and Skoog (MS) medium containing 5 mgL-1e5
- Outcome
- shoot regeneration regenerated from green spots and numbers of plantlet with complete shoot/root developmente5
- Direction
- higheste5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Balinese red rice (Oryza sativa Cv. Barak Cenana)”
e5“The highest number of shoot regeneration regenerated from green spots and numbers of plantlet with complete shoot/root development were observed when transferred calli to Murashige and Skoog (MS) medium containing 5 mgL-1”
Claim · model-written summary
Regenerated plantlets formed multiple shoots, described as an efficient regeneration system.
- Taxon
- Oryza sativaOryza sativat0
- Outcome
- Multiple shoot formation of regenerated plantletse6
- Qualifier
- efficient regeneration systeme6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Balinese red rice (Oryza sativa Cv. Barak Cenana)”
e6“Multiple shoot formation of regenerated plantlets revealed for efficient regeneration system, which was firstly reported in this present study”
The same questions in other papers
in vitro · growth · Oryza sativa (rice)Positive and negative results
139 papers in the same direction as this paper · 24 in the opposite direction · 1 reporting no difference from an alternative
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