Paper · 1969 · American Journal of Botany

A Histochemical Study of Abscission Layer Formation in the Bean

H. P. Rasmussen; M. J. Bukovac

11
claims
21
verbatim quotes
13
citations
2
research areas
✓
Open access

Abstract

In debladed bean petioles calcium and dry weight increased in the abscission zone during an induction period of 14 hr. Before the microscopic appearance of the abscission layer calcium decreased in the abscission zone and increased in the petiole. Dry matter began to decrease in both the abscission zone and the petiole 24 hr after deblading. The first visual change in the cells of the abscission zone was a swelling of the pectic materials of the cell walls. This was followed by breakdown of other cell wall components, i.e., non-cellulosic polysaccharides and cellulose. The cellulose of the cell walls adjacent and distal to the abscission layer was found to be altered; however, no lignin was present during abscission layer development. The alteration of pectic materials, coupled with breakdown of cell wall components, resulted in the collapse of cells of the abscission layer just prior to separation. Auxin delayed abscission and also delayed the initial increase in calcium, the movement of calcium from the abscission zone to the petiole, and the decrease in dry weight.

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Claims 11

Observationobservedoriginal resultmeasured

Claim · model-written summary

In debladed bean petioles, calcium and dry weight increased in the abscission zone during a 14 hr induction period.

Taxon
beane1
Organ / stage
abscission zonee1
Treatment
debladede1
Outcome
calcium and dry weighte1
Direction
increasede1
Qualifier
during an induction period of 14 hre1

Verbatim

e1

“In debladed bean petioles calcium and dry weight increased in the abscission zone during an induction period of 14 hr”

✓ located verbatim in the source · abstract, characters 0–117 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Before the abscission layer appeared microscopically, calcium decreased in the abscission zone.

Taxon
Beant0
Organ / stage
abscission zonee2
Outcome
calciume2
Direction
decreasede2
Qualifier
Before the microscopic appearance of the abscission layere2

Verbatim

e2

“Before the microscopic appearance of the abscission layer calcium decreased in the abscission zone”

✓ located verbatim in the source · abstract, characters 119–217 · show in text ↑
t0

“Abscission Layer Formation in the Bean”

✓ located verbatim in the source · title, characters 25–63 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Before the abscission layer appeared microscopically, calcium increased in the petiole.

Taxon
Beant0
Organ / stage
petiolee2
Outcome
calciume2
Direction
increasede2
Qualifier
Before the microscopic appearance of the abscission layere2

Verbatim

e2

“Before the microscopic appearance of the abscission layer calcium decreased in the abscission zone and increased in the petiole”

✓ located verbatim in the source · abstract, characters 119–246 · show in text ↑
t0

“Abscission Layer Formation in the Bean”

✓ located verbatim in the source · title, characters 25–63 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Dry matter began to decrease in both the abscission zone and the petiole 24 hr after deblading.

Taxon
Beant0
Organ / stage
abscission zone and the petiolee3
Treatment
debladinge3
Outcome
dry mattere3
Direction
decreasee3
Qualifier
24 hr after debladinge3

Verbatim

e3

“Dry matter began to decrease in both the abscission zone and the petiole 24 hr after deblading”

✓ located verbatim in the source · abstract, characters 248–342 · show in text ↑
t0

“Abscission Layer Formation in the Bean”

✓ located verbatim in the source · title, characters 25–63 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The first visual change in the cells of the abscission zone was swelling of the pectic materials of the cell walls.

Taxon
Beant0
Organ / stage
cells of the abscission zonee4
Outcome
swelling of the pectic materials of the cell wallse4

Verbatim

e4

“The first visual change in the cells of the abscission zone was a swelling of the pectic materials of the cell walls”

✓ located verbatim in the source · abstract, characters 344–460 · show in text ↑
t0

“Abscission Layer Formation in the Bean”

✓ located verbatim in the source · title, characters 25–63 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Breakdown of other cell wall components followed the swelling; the components were non-cellulosic polysaccharides and cellulose.

Taxon
Beant0
Outcome
breakdown of other cell wall componentse5
Qualifier
non-cellulosic polysaccharides and cellulosee5

Verbatim

e5

“This was followed by breakdown of other cell wall components, i.e., non-cellulosic polysaccharides and cellulose”

✓ located verbatim in the source · abstract, characters 462–574 · show in text ↑
t0

“Abscission Layer Formation in the Bean”

✓ located verbatim in the source · title, characters 25–63 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Cellulose of the cell walls adjacent and distal to the abscission layer was altered.

Taxon
Beant0
Organ / stage
cell walls adjacent and distal to the abscission layere6
Outcome
cellulosee6
Qualifier
was found to be alterede6

Verbatim

e6

“The cellulose of the cell walls adjacent and distal to the abscission layer was found to be altered”

✓ located verbatim in the source · abstract, characters 576–675 · show in text ↑
t0

“Abscission Layer Formation in the Bean”

✓ located verbatim in the source · title, characters 25–63 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

No lignin was present during abscission layer development.

Taxon
Beant0
Outcome
lignine7
Qualifier
during abscission layer developmente7

Verbatim

e7

“no lignin was present during abscission layer development”

✓ located verbatim in the source · abstract, characters 686–743 · show in text ↑
t0

“Abscission Layer Formation in the Bean”

✓ located verbatim in the source · title, characters 25–63 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Alteration of pectic materials, coupled with breakdown of cell wall components, resulted in collapse of abscission layer cells just prior to separation.

Taxon
Beant0
Organ / stage
abscission layere5
Outcome
collapse of cells of the abscission layere5
Qualifier
alteration of pectic materials, coupled with breakdown of cell wall componentse5

Verbatim

e5

“The alteration of pectic materials, coupled with breakdown of cell wall components, resulted in the collapse of cells of the abscission layer just prior to separation”

✓ located verbatim in the source · abstract, characters 745–911 · show in text ↑
t0

“Abscission Layer Formation in the Bean”

✓ located verbatim in the source · title, characters 25–63 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Auxin delayed abscission.

Taxon
Beant0
Treatment
Auxine6
Outcome
abscissione6
Direction
delayede6

Not reportedComparator

Verbatim

e6

“Auxin delayed abscission”

✓ located verbatim in the source · abstract, characters 913–937 · show in text ↑
t0

“Abscission Layer Formation in the Bean”

✓ located verbatim in the source · title, characters 25–63 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Auxin delayed the initial increase in calcium, the movement of calcium from the abscission zone to the petiole, and the decrease in dry weight.

Taxon
Beant0
Treatment
Auxine7
Outcome
the initial increase in calcium, the movement of calcium from the abscission zone to the petiole, and the decrease in dry weighte7
Direction
delayede7

Not reportedComparator

Verbatim

e7

“Auxin delayed abscission and also delayed the initial increase in calcium, the movement of calcium from the abscission zone to the petiole, and the decrease in dry weight”

✓ located verbatim in the source · abstract, characters 913–1083 · show in text ↑
t0

“Abscission Layer Formation in the Bean”

✓ located verbatim in the source · title, characters 25–63 · show in text ↑