Abstract
We have identified nine oligopeptide transporter (OPT) orthologs (AtOPT1 to AtOPT9) in Arabidopsis. These proteins show significant sequence similarity to OPTs of Candida albicans (CaOpt1p), Schizosaccharomyces pombe (Isp4p), and Saccharomyces cerevisiae (Opt1p and Opt2p). Hydrophilicity plots of the OPTs suggest that they are integral membrane proteins with 12 to 14 transmembrane domains. Sequence comparisons showed that the AtOPTs form a distinct subfamily when compared with the fungal OPTs. Two highly conserved motifs (NPG and KIPPR) were found among all OPT members. The identification of multiple OPTs in Arabidopsis suggests that they may play different functional roles. This idea is supported by the fact that AtOPTs have a distinct, tissue-specific expression pattern. The cDNAs encoding seven of the AtOPTs were cloned into a yeast vector under the control of a constitutive promoter. AtOPT4 expressed in S. cerevisiae mediated the uptake of KLG-[ 3 H]L. Similarly, expression of five of the seven AtOPT proteins expressed in yeast conferred the ability to uptake tetraand pentapeptides as measured by growth. This study provides new evidence for multiple peptide transporter systems in Arabidopsis, suggesting an important physiological role for small peptides in plants.
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Claims 8
Claim · model-written summary
Nine oligopeptide transporter orthologs (AtOPT1 to AtOPT9) were identified in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise1 - Gene
- AtOPT1 to AtOPT9e1
- Outcome
- oligopeptide transporter (OPT) orthologse1
- Value
- ninee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We have identified nine oligopeptide transporter (OPT) orthologs (AtOPT1 to AtOPT9) in Arabidopsis”
Claim · model-written summary
The AtOPTs form a distinct subfamily compared with fungal OPTs.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AtOPTse2
- Outcome
- distinct subfamilye2
- Comparator
- fungal OPTse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“An Oligopeptide Transporter Gene Family in Arabidopsis”
e2“Sequence comparisons showed that the AtOPTs form a distinct subfamily when compared with the fungal OPTs”
Claim · model-written summary
Two highly conserved motifs (NPG and KIPPR) were found among all OPT members.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- NPG and KIPPRe3
- Outcome
- highly conserved motifse3
- Value
- Twoe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“An Oligopeptide Transporter Gene Family in Arabidopsis”
e3“Two highly conserved motifs (NPG and KIPPR) were found among all OPT members”
Claim · model-written summary
The AtOPTs have a distinct, tissue-specific expression pattern.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AtOPTse4
- Outcome
- expression patterne4
- Qualifier
- tissue-specifice4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“An Oligopeptide Transporter Gene Family in Arabidopsis”
e4“This idea is supported by the fact that AtOPTs have a distinct, tissue-specific expression pattern”
Claim · model-written summary
AtOPT4 expressed in yeast mediated uptake of KLG-[3H]L.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- expressed in S. cerevisiaee5
- Gene
- AtOPT4e5
- Outcome
- uptake of KLG-[ 3 H]Le5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“An Oligopeptide Transporter Gene Family in Arabidopsis”
e5“AtOPT4 expressed in S. cerevisiae mediated the uptake of KLG-[ 3 H]L”
Claim · model-written summary
Expression of five of the seven AtOPT proteins in yeast conferred the ability to take up tetra- and pentapeptides, as measured by growth.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- expression of five of the seven AtOPT proteins expressed in yeaste6
- Gene
- AtOPT proteinse6
- Outcome
- ability to uptake tetraand pentapeptidese6
- Value
- five of the sevene6
- Qualifier
- as measured by growthe6
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“An Oligopeptide Transporter Gene Family in Arabidopsis”
e6“expression of five of the seven AtOPT proteins expressed in yeast conferred the ability to uptake tetraand pentapeptides as measured by growth”
Claim · model-written summary
This study provides new evidence for multiple peptide transporter systems in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise7
- Outcome
- multiple peptide transporter systemse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“This study provides new evidence for multiple peptide transporter systems in Arabidopsis, suggesting an important physiological role for small peptides in plants”
Claim · model-written summary
Small peptides may have an important physiological role in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise7
- Compound
- small peptidese7
- Outcome
- an important physiological role for small peptidese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“This study provides new evidence for multiple peptide transporter systems in Arabidopsis, suggesting an important physiological role for small peptides in plants”
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