Abstract
Virus-host coevolution often drives virus immune escape. However, it remains unknown whether natural variations of plant virus resistance are enriched in genes of RNA interference (RNAi) pathway known to confer essential antiviral defense in plants. Here, we report two genome-wide association study screens to interrogate natural variation among wild-collected Arabidopsis thaliana accessions in quantitative resistance to the endemic cucumber mosaic virus (CMV). We demonstrate that the highest-ranked gene significantly associated with resistance from both screens acts to regulate antiviral RNAi in ecotype Columbia-0. One gene, corresponding to Reduced Dormancy 5 (RDO5), enhances resistance by promoting amplification of the virus-derived small interfering RNAs (vsiRNAs). Interestingly, the second gene, designated Antiviral RNAi Regulator 1 (VIR1), dampens antiviral RNAi so its genetic inactivation by CRISPR/Cas9 editing enhances both vsiRNA production and CMV resistance. Our findings identify positive and negative regulators of the antiviral RNAi defense that may play important roles in virus-host coevolution.
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Claims 5
Claim · model-written summary
The top gene from both association screens regulated antiviral RNA interference.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Material
- ecotype Columbia-0e0
- Gene
- highest-ranked genee0
- Outcome
- resistancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We demonstrate that the highest-ranked gene significantly associated with resistance from both screens acts to regulate antiviral RNAi in ecotype Columbia-0.”
t0“Identification of positive and negative regulators of antiviral RNA interference in Arabidopsis thaliana”
Claim · model-written summary
RDO5 promoted viral small interfering RNA amplification and enhanced resistance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- Reduced Dormancy 5 (RDO5)e0
- Outcome
- resistancee0
- Direction
- enhancese0
- Qualifier
- promoting amplification of the virus-derived small interfering RNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One gene, corresponding to Reduced Dormancy 5 (RDO5), enhances resistance by promoting amplification of the virus-derived small interfering RNAs (vsiRNAs).”
t0“Identification of positive and negative regulators of antiviral RNA interference in Arabidopsis thaliana”
Claim · model-written summary
VIR1 dampened antiviral RNA interference.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- Antiviral RNAi Regulator 1 (VIR1)e0
- Outcome
- antiviral RNAie0
- Direction
- dampense0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interestingly, the second gene, designated Antiviral RNAi Regulator 1 (VIR1), dampens antiviral RNAi so its genetic inactivation by CRISPR/Cas9 editing enhances both vsiRNA production and CMV resistance.”
t0“Identification of positive and negative regulators of antiviral RNA interference in Arabidopsis thaliana”
Claim · model-written summary
CRISPR/Cas9 inactivation of VIR1 enhanced viral small interfering RNA production.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- genetic inactivation by CRISPR/Cas9 editinge0
- Gene
- Antiviral RNAi Regulator 1 (VIR1)e0
- Outcome
- vsiRNA productione0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interestingly, the second gene, designated Antiviral RNAi Regulator 1 (VIR1), dampens antiviral RNAi so its genetic inactivation by CRISPR/Cas9 editing enhances both vsiRNA production and CMV resistance.”
t0“Identification of positive and negative regulators of antiviral RNA interference in Arabidopsis thaliana”
Claim · model-written summary
CRISPR/Cas9 inactivation of VIR1 enhanced CMV resistance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- genetic inactivation by CRISPR/Cas9 editinge0
- Gene
- Antiviral RNAi Regulator 1 (VIR1)e0
- Outcome
- CMV resistancee0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interestingly, the second gene, designated Antiviral RNAi Regulator 1 (VIR1), dampens antiviral RNAi so its genetic inactivation by CRISPR/Cas9 editing enhances both vsiRNA production and CMV resistance.”
t0“Identification of positive and negative regulators of antiviral RNA interference in Arabidopsis thaliana”
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