Abstract
The novel exopolysaccharide EPS-E8, secreted by Pediococcus pentosaceus E8, was obtained by anion-exchange and gel filtration chromatography. Structural analyses identified EPS-E8 as a heteropolysaccharide containing mannose, glucose, and galactose. Its major backbone consists of →2)-α- D -Man p -(1→2,6)-α- D -Glc p -(1→6)-α- D -Man p -(1→, and its molecular weight is 5.02 × 10 4 g/mol. Using atomic force microscopy and scanning electron microscopy, many spherical and irregular reticular-like shapes were observed in the microstructure of EPS-E8. EPS-E8 has outstanding thermal stability (305.7°C). Both the zeta potential absolute value and average particle diameter increased gradually with increasing concentration. Moreover, at a concentration of 10 mg/ml, the antioxidant capacities of, 1-Diphenyl-2-picrylhydrazyl (DPPH), ABTS and hydroxyl radical were 50.62 ± 0.5%, 52.17 ± 1.4%, and 58.91 ± 0.7%, respectively. EPS-E8 possesses excellent emulsifying properties against several food-grade oils, and its activity is retained under various conditions (temperature, pH, and ionic strength). Finally, we found that EPS-E8 as a polysaccharide-based coating could reduce the weight loss and malondialdehyde (MDA) content of strawberry, as well as preserving the vitamin C and soluble solid content during storage at 20°C. Together, the results support the potential application of EPS-E8 as an emulsifier, and a polysaccharide-based coating in fruit preservation.
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Claims 13
Claim · model-written summary
EPS-E8 is a heteropolysaccharide containing mannose, glucose and galactose.
- Outcome
- heteropolysaccharide containing mannose, glucose, and galactosee1
Not reportedTaxon
Verbatim
e1“Structural analyses identified EPS-E8 as a heteropolysaccharide containing mannose, glucose, and galactose”
Claim · model-written summary
EPS-E8 has a molecular weight of 5.02 × 10 4 g/mol.
Not reportedTaxon
Verbatim
e1“its molecular weight is 5.02 × 10 4 g/mol”
Claim · model-written summary
EPS-E8 has thermal stability of 305.7°C.
Not reportedTaxon
Verbatim
e1“EPS-E8 has outstanding thermal stability (305.7°C)”
Claim · model-written summary
At 10 mg/ml, EPS-E8 showed DPPH antioxidant capacity of 50.62 ± 0.5%.
Not reportedTaxon
Verbatim
e1“Moreover, at a concentration of 10 mg/ml, the antioxidant capacities of, 1-Diphenyl-2-picrylhydrazyl (DPPH), ABTS and hydroxyl radical were 50.62 ± 0.5%, 52.17 ± 1.4%, and 58.91 ± 0.7%, respectively”
Claim · model-written summary
At 10 mg/ml, EPS-E8 showed ABTS antioxidant capacity of 52.17 ± 1.4%.
Not reportedTaxon
Verbatim
e1“Moreover, at a concentration of 10 mg/ml, the antioxidant capacities of, 1-Diphenyl-2-picrylhydrazyl (DPPH), ABTS and hydroxyl radical were 50.62 ± 0.5%, 52.17 ± 1.4%, and 58.91 ± 0.7%, respectively”
Claim · model-written summary
At 10 mg/ml, EPS-E8 showed hydroxyl radical antioxidant capacity of 58.91 ± 0.7%.
Not reportedTaxon
Verbatim
e1“Moreover, at a concentration of 10 mg/ml, the antioxidant capacities of, 1-Diphenyl-2-picrylhydrazyl (DPPH), ABTS and hydroxyl radical were 50.62 ± 0.5%, 52.17 ± 1.4%, and 58.91 ± 0.7%, respectively”
Claim · model-written summary
EPS-E8 has excellent emulsifying properties against several food-grade oils.
Not reportedTaxon
Verbatim
e1“EPS-E8 possesses excellent emulsifying properties against several food-grade oils”
Claim · model-written summary
Emulsifying activity of EPS-E8 is retained under various temperature, pH and ionic strength conditions.
Not reportedTaxon
Verbatim
e1“its activity is retained under various conditions (temperature, pH, and ionic strength)”
Claim · model-written summary
Zeta potential absolute value and average particle diameter of EPS-E8 increased with increasing concentration.
- Treatment
- increasing concentratione1
- Outcome
- zeta potential absolute value and average particle diametere1
- Direction
- increasede1
Not reportedTaxon
Verbatim
e1“Both the zeta potential absolute value and average particle diameter increased gradually with increasing concentration”
Claim · model-written summary
Many spherical and irregular reticular-like shapes were observed in the microstructure of EPS-E8.
- Outcome
- spherical and irregular reticular-like shapese1
Not reportedTaxon
Verbatim
e1“many spherical and irregular reticular-like shapes were observed in the microstructure of EPS-E8”
Claim · model-written summary
EPS-E8 has a major backbone structure.
- Outcome
- major backbonee1
Not reportedTaxon
Verbatim
e1“Its major backbone consists of”
Claim · model-written summary
EPS-E8 coating could reduce weight loss and MDA content of strawberry.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae1 - Material
- EPS-E8e1
- Treatment
- polysaccharide-based coatinge1
- Outcome
- weight loss and malondialdehyde (MDA) contente1
- Direction
- reducee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e1“Finally, we found that EPS-E8 as a polysaccharide-based coating could reduce the weight loss and malondialdehyde (MDA) content of strawberry, as well as preserving the vitamin C and soluble solid content during storage at 20°C”
Claim · model-written summary
EPS-E8 coating preserved vitamin C and soluble solid content of strawberry during storage at 20°C.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- polysaccharide-based coatinge1
- Outcome
- vitamin C and soluble solid contente1
- Direction
- preservinge1
- Setting
- storage at 20°Ce1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Finally, we found that EPS-E8 as a polysaccharide-based coating could reduce the weight loss and malondialdehyde (MDA) content of strawberry, as well as preserving the vitamin C and soluble solid content during storage at 20°C”
The same questions in other papers
storage · physiology: oxidative · Fragaria x ananassa (strawberry)Positive and negative results
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- negative
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38 papers in the same direction as this paper · 9 in the opposite direction
- positive
- no effect
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See all 48 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.