Abstract
Aluminum (Al) toxicity causes severe reduction in crop yields in acidic soil. The natural resistance-associated macrophage proteins (NRAMPs) play an important role in the transport of mineral elements in plants. Recently, OsNrat1 and SbNrat1 were reported specifically to transport trivalent Al ions. In this study, we functionally characterized ZmNRAMP4, a gene previously identified from RNA-Seq data from Al-treated maize roots, in response to Al exposure in maize. ZmNRAMP4 was predominantly expressed in root tips and was specifically induced by Al stress. Yeast cells expressing ZmNRAMP4 were hypersensitive to Al, which was associated with Al accumulation in yeast. Furthermore, overexpression of ZmNRAMP4 in Arabidopsis conferred transgenic plants with a significant increase in Al tolerance. However, expression of ZmNRAMP4, either in yeast or in Arabidopsis, had no effect on the response to cadmium stress. Taken together, these results underlined an internal tolerance mechanism involving ZmNRAMP4 to enhance Al tolerance via cytoplasmic sequestration of Al in maize.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
ZmNRAMP4 was predominantly expressed in maize root tips.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified from RNA-Seq data from Al-treated maize roots”
e1“ZmNRAMP4 was predominantly expressed in root tips and was specifically induced by Al stress”
Claim · model-written summary
Aluminum stress specifically induced ZmNRAMP4 in maize.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified from RNA-Seq data from Al-treated maize roots”
e1“ZmNRAMP4 was predominantly expressed in root tips and was specifically induced by Al stress”
Claim · model-written summary
Yeast cells expressing ZmNRAMP4 were hypersensitive to Al.
- Material
- Yeast cellse2
- Treatment
- expressing ZmNRAMP4e2
- Gene
- ZmNRAMP4e2
- Outcome
- hypersensitive to Ale2
- Direction
- hypersensitivee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified from RNA-Seq data from Al-treated maize roots”
e2“Yeast cells expressing ZmNRAMP4 were hypersensitive to Al, which was associated with Al accumulation in yeast”
Claim · model-written summary
Hypersensitivity of ZmNRAMP4-expressing yeast was associated with Al accumulation in yeast.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified from RNA-Seq data from Al-treated maize roots”
e2“Yeast cells expressing ZmNRAMP4 were hypersensitive to Al, which was associated with Al accumulation in yeast”
Claim · model-written summary
Overexpression of ZmNRAMP4 in Arabidopsis significantly increased Al tolerance.
- Taxon
- Arabidopsis
Arabidopsise3 - Treatment
- overexpression of ZmNRAMP4e3
- Gene
- ZmNRAMP4e3
- Outcome
- Al tolerancee3
- Direction
- increasee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“overexpression of ZmNRAMP4 in Arabidopsis conferred transgenic plants with a significant increase in Al tolerance”
Claim · model-written summary
Expression of ZmNRAMP4 in yeast or Arabidopsis had no effect on the response to cadmium stress.
- Taxon
- ArabidopsisArabidopsise4
- Treatment
- expression of ZmNRAMP4e4
- Gene
- ZmNRAMP4e4
- Outcome
- response to cadmium stresse4
- Direction
- no effecte4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“expression of ZmNRAMP4, either in yeast or in Arabidopsis, had no effect on the response to cadmium stress”
Claim · model-written summary
ZmNRAMP4 enhances Al tolerance in maize by cytoplasmic sequestration of Al.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“an internal tolerance mechanism involving ZmNRAMP4 to enhance Al tolerance via cytoplasmic sequestration of Al in maize”
The same questions in other papers
transgenic · nutrient: content · ArabidopsisPositive and negative results
5 papers in the same direction as this paper · 11 in the opposite direction
- positive
- positive
- positive
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.