Abstract
The relevance of non-Saccharomyces native yeasts for improving the complexity of wine aroma is increasingly recognised, besides their use in a range of biotechnological applications. Previously, we used a metagenomic approach to assess the effects of vineyard calcium sprays on the microbiota of grape berries cv. Vinhão and Loureiro. In this study, we aimed at assembling a catalogue of the cultivable yeasts from grape berries of these cultivars and to investigate the direct effect of calcium and other elicitors on the isolated taxa. Seventeen unique colony morphologies were identified, and the sequencing of the region spanning the rDNA internal transcribed spacers or the rDNA D1/D2 domain revealed that they are distributed into 13 different genera. Although the cultivar and vintage influenced the taxa abundance and diversity, Aureobasidium pullulans and Hanseniaspora uvarum were the most abundant species, ubiquitously populating the fruits together with Pichia terricola, Rhodotorula glutinis, Starmerella bacillaris and Vishniacozyma heimaeyensis. The rarest genera comprised Candida, Trichosporon and Zygoascus. Analysis of yeast growth in the presence of specific elicitors revealed that Pichia spp. are the most sensitive to exogenous calcium, while Sporobolomyces roseus is the most sensitive to copper exposure. Moreover, A. pullulans, H. uvarum and S. bacillaris displayed the highest sensitivity to osmotic stress. In turn, Wickerhamomyces anomalus, Metschnikowia pulcherrima, P. fermentans, P. kluyveri and P. kudriavzevii showed mild resistance to ethanol, unlike the remaining non-Saccharomyces yeasts. Correlation networks expressing the overall yeast performance showed that H. uvarum and S. bacillaris share the strongest positive interspecies correlation, followed by the pairs P. fermentans–P. kluyveri, B. albus–R. glutinis and A. pullulans–V. heimaeyensis. The strongest negative correlations were found for the pairs P. kluyveri–S. roseus, P. terricola–W. anomalus, P. kudriavzevii–V. heimayensis, P. kluyveri–V. heimayensis, and A. pullulans–P. kluyveri. This study exposed microbial niches with a predictably common response to abiotic stress.
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Claims 10
Claim · model-written summary
Seventeen cultivable yeast colony morphologies from grape berries fell into 13 genera.
Verbatim
e0“In this study, we aimed at assembling a catalogue of the cultivable yeasts from grape berries of these cultivars and to investigate the direct effect of calcium and other elicitors on the isolated taxa”
e1“Seventeen unique colony morphologies were identified, and the sequencing of the region spanning the rDNA internal transcribed spacers or the rDNA D1/D2 domain revealed that they are distributed into 13 different genera”
Claim · model-written summary
Aureobasidium pullulans and Hanseniaspora uvarum were the most abundant yeasts on grape berries.
- Taxon
- grape berriese0
- Interactor
- Aureobasidium pullulans
Aureobasidium pullulanse1other - Second interactor
- Hanseniaspora uvarumHanseniaspora uvarume1other
- Outcome
- the most abundant speciese1
- Direction
- most abundante1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In this study, we aimed at assembling a catalogue of the cultivable yeasts from grape berries of these cultivars and to investigate the direct effect of calcium and other elicitors on the isolated taxa”
e1“Aureobasidium pullulans and Hanseniaspora uvarum were the most abundant species”
Claim · model-written summary
Cultivar and vintage influenced yeast taxa abundance and diversity on grape berries.
Verbatim
e0“In this study, we aimed at assembling a catalogue of the cultivable yeasts from grape berries of these cultivars and to investigate the direct effect of calcium and other elicitors on the isolated taxa”
e1“Although the cultivar and vintage influenced the taxa abundance and diversity”
Claim · model-written summary
Pichia spp. were the most sensitive yeasts to exogenous calcium in growth tests.
PichiaNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In this study, we aimed at assembling a catalogue of the cultivable yeasts from grape berries of these cultivars and to investigate the direct effect of calcium and other elicitors on the isolated taxa”
e1“Analysis of yeast growth in the presence of specific elicitors revealed that Pichia spp. are the most sensitive to exogenous calcium, while Sporobolomyces roseus is the most sensitive to copper exposure”
Claim · model-written summary
Sporobolomyces roseus was the most sensitive yeast to copper exposure in growth tests.
- Taxon
- grape berriese0
- Treatment
- copper exposuree1
- Interactor
- Sporobolomyces roseusSporobolomyces roseuse1other
- Outcome
- yeast growthe1
- Direction
- most sensitivee1
Not reportedComparator
Verbatim
e0“In this study, we aimed at assembling a catalogue of the cultivable yeasts from grape berries of these cultivars and to investigate the direct effect of calcium and other elicitors on the isolated taxa”
e1“Analysis of yeast growth in the presence of specific elicitors revealed that Pichia spp. are the most sensitive to exogenous calcium, while Sporobolomyces roseus is the most sensitive to copper exposure”
Claim · model-written summary
Aureobasidium pullulans, Hanseniaspora uvarum and Starmerella bacillaris had the highest sensitivity to osmotic stress.
- Taxon
- grape berriese0
- Treatment
- osmotic stresse1
- Interactor
- A. pullulansAureobasidium pullulanse1other
- Second interactor
- H. uvarumHanseniaspora uvarume1other
- Outcome
- sensitivitye1
- Direction
- higheste1
- Qualifier
- S. bacillarise1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In this study, we aimed at assembling a catalogue of the cultivable yeasts from grape berries of these cultivars and to investigate the direct effect of calcium and other elicitors on the isolated taxa”
e1“A. pullulans, H. uvarum and S. bacillaris displayed the highest sensitivity to osmotic stress”
Claim · model-written summary
Wickerhamomyces anomalus and Metschnikowia pulcherrima showed mild resistance to ethanol, unlike the remaining non-Saccharomyces yeasts.
Verbatim
e0“In this study, we aimed at assembling a catalogue of the cultivable yeasts from grape berries of these cultivars and to investigate the direct effect of calcium and other elicitors on the isolated taxa”
e1“In turn, Wickerhamomyces anomalus, Metschnikowia pulcherrima, P. fermentans, P. kluyveri and P. kudriavzevii showed mild resistance to ethanol, unlike the remaining non-Saccharomyces yeasts”
Claim · model-written summary
Hanseniaspora uvarum and Starmerella bacillaris had the strongest positive interspecies correlation.
- Taxon
- grape berriese0
- Interactor
- H. uvarumHanseniaspora uvarume1other
- Second interactor
- S. bacillarisStarmerella bacillarise1other
- Outcome
- interspecies correlatione1
- Direction
- strongest positivee1
Verbatim
e0“In this study, we aimed at assembling a catalogue of the cultivable yeasts from grape berries of these cultivars and to investigate the direct effect of calcium and other elicitors on the isolated taxa”
e1“H. uvarum and S. bacillaris share the strongest positive interspecies correlation”
Claim · model-written summary
The strongest negative yeast correlations were found for pairs including Pichia kluyveri and Sporobolomyces roseus.
- Taxon
- grape berriese0
- Interactor
- P. kluyveriPichia kluyverie1other
- Second interactor
- S. roseusSporobolomyces roseuse1other
- Outcome
- correlationse1
- Direction
- strongest negativee1
Verbatim
e0“In this study, we aimed at assembling a catalogue of the cultivable yeasts from grape berries of these cultivars and to investigate the direct effect of calcium and other elicitors on the isolated taxa”
e1“The strongest negative correlations were found for the pairs P. kluyveri–S. roseus, P. terricola–W. anomalus, P. kudriavzevii–V. heimayensis, P. kluyveri–V. heimayensis, and A. pullulans–P. kluyveri”
Claim · model-written summary
Microbial niches on grape berries show a predictably common response to abiotic stress.
Verbatim
e0“In this study, we aimed at assembling a catalogue of the cultivable yeasts from grape berries of these cultivars and to investigate the direct effect of calcium and other elicitors on the isolated taxa”
e1“This study exposed microbial niches with a predictably common response to abiotic stress”