Abstract
The cotton chromosome substitution line, CS-B15sh, exhibits 41% lower injury from 2,4-D when applied at the field recommended rate of 1.12 kg ae ha −1 (1×) than does Texas Marker-1 (TM-1). CS-B15sh was developed in the genetic background of Gossypium hirsutum L. cv TM-1 and has chromosome introgression on the short arm of chromosome 15 from Gossypium barbadense L. cv. Pima 379. In a previous experiment, we observed reduced translocation of [ 14 C]2,4-D outside the treated leaf tissue in CS-B15sh, which contrasted with an increased translocation of the herbicide in the tissues above and below the treated leaf in TM-1. Our results indicate a potential 2,4-D tolerance mechanism in CS-B15sh involving altered movement of 2,4-D. Here, we used RNA sequencing (RNA-seq) to determine the differential expression of genes between 2,4-D-challenged and control plants of the tolerant (CS-B15sh) and susceptible lines (TM-1 and Pima 379). Several components of the 2,4-D/auxin-response pathway—including ubiquitin E3 ligase, PB1|AUX/IAA, ARF transcription factors, and F-box proteins of the SCF TIR1/AFB complex—were upregulated with at least threefold higher expression in TM-1 compared with CS-B15sh, while both Pima 379 and TM-1 showed the same fold change expression for PB1|AUX/IAA mRNA. Some genes associated with herbicide metabolism, including flavin monooxygenase (Gohir.A01G174100) and FAD-linked oxidase (Gohir.D06G002600), exhibited at least a twofold increase in CS-B15sh than in TM-1 (the gene was not expressed in Pima 379), suggesting a potential relationship between the gene’s expression and 2,4-D tolerance. It is interesting to note that glutathione S-transferase was differentially expressed in both CS-B15sh and Pima 379 but not in TM-1, while cytochrome P450 and other genes involved in the oxidation–reduction process were significantly expressed only in CS-B15sh in response to 2,4-D. Gene set enrichment analysis on the union DEGs of the three cotton genotypes revealed the depletion of transcripts involved in photosynthesis and enrichment of transcripts involved in ABA response and signaling.
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Claims 13
Claim · model-written summary
CS-B15sh had 41% less 2,4-D injury than Texas Marker-1.
- Taxon
- cotton
Gossypium hirsutume0 - Material
- CS-B15she0
- Treatment
- 2,4-D when applied at the field recommended rate of 1.12 kg ae ha −1 (1×)e0
- Outcome
- injury from 2,4-De0
- Direction
- lowere0
- Value
- 41%e0
- Comparator
- Texas Marker-1 (TM-1)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cotton chromosome substitution line, CS-B15sh, exhibits 41% lower injury from 2,4-D when applied at the field recommended rate of 1.12 kg ae ha −1 (1×) than does Texas Marker-1 (TM-1)”
Claim · model-written summary
CS-B15sh showed reduced movement of labeled 2,4-D outside treated leaves.
- Taxon
- cottonGossypium hirsutume1
- Material
- CS-B15she0
- Outcome
- translocation of [ 14 C]2,4-D outside the treated leaf tissuee0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we observed reduced translocation of [ 14 C]2,4-D outside the treated leaf tissue in CS-B15sh”
e1“The cotton chromosome substitution line, CS-B15sh”
Claim · model-written summary
TM-1 showed increased 2,4-D movement above and below treated leaves.
- Taxon
- cottonGossypium hirsutume1
- Material
- TM-1e0
- Outcome
- translocation of the herbicidee0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased translocation of the herbicide in the tissues above and below the treated leaf in TM-1”
e1“The cotton chromosome substitution line, CS-B15sh”
Claim · model-written summary
Altered 2,4-D movement was proposed as a tolerance mechanism in CS-B15sh.
- Taxon
- cottonGossypium hirsutume1
- Material
- CS-B15she0
- Outcome
- 2,4-D tolerancee0
- Qualifier
- altered movement of 2,4-De0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a potential 2,4-D tolerance mechanism in CS-B15sh involving altered movement of 2,4-D”
e1“The cotton chromosome substitution line, CS-B15sh”
Claim · model-written summary
Auxin-response pathway components were at least threefold more expressed in TM-1 than CS-B15sh.
- Taxon
- cottonGossypium hirsutume1
- Material
- TM-1e0
- Gene
- ubiquitin E3 ligase, PB1|AUX/IAA, ARF transcription factorse0
- Outcome
- expressione0
- Direction
- highere0
- Value
- at least threefolde0
- Comparator
- CS-B15she0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ubiquitin E3 ligase, PB1|AUX/IAA, ARF transcription factors, and F-box proteins of the SCF TIR1/AFB complex—were upregulated with at least threefold higher expression in TM-1 compared with CS-B15sh”
e1“The cotton chromosome substitution line, CS-B15sh”
Claim · model-written summary
PB1|AUX/IAA mRNA had the same fold-change expression in Pima 379 and TM-1.
- Taxon
- cottonGossypium hirsutume1
- Material
- Pima 379e0
- Gene
- PB1|AUX/IAA mRNAe0
- Outcome
- fold change expressione0
- Direction
- samee0
- Comparator
- TM-1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both Pima 379 and TM-1 showed the same fold change expression for PB1|AUX/IAA mRNA”
e1“The cotton chromosome substitution line, CS-B15sh”
Claim · model-written summary
Flavin monooxygenase expression was at least twofold higher in CS-B15sh than TM-1.
- Taxon
- cottonGossypium hirsutume1
- Material
- CS-B15she0
- Gene
- flavin monooxygenase (Gohir.A01G174100)e0
- Outcome
- increasee0
- Direction
- increasee0
- Value
- at least a twofolde0
- Comparator
- TM-1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“flavin monooxygenase (Gohir.A01G174100) and FAD-linked oxidase (Gohir.D06G002600), exhibited at least a twofold increase in CS-B15sh than in TM-1 (the gene was not expressed in Pima 379)”
e1“The cotton chromosome substitution line, CS-B15sh”
Claim · model-written summary
FAD-linked oxidase expression was at least twofold higher in CS-B15sh than TM-1.
- Taxon
- cottonGossypium hirsutume1
- Material
- CS-B15she0
- Gene
- FAD-linked oxidase (Gohir.D06G002600)e0
- Outcome
- increasee0
- Direction
- increasee0
- Value
- at least a twofolde0
- Comparator
- TM-1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“flavin monooxygenase (Gohir.A01G174100) and FAD-linked oxidase (Gohir.D06G002600), exhibited at least a twofold increase in CS-B15sh than in TM-1 (the gene was not expressed in Pima 379)”
e1“The cotton chromosome substitution line, CS-B15sh”
Claim · model-written summary
The gene was not expressed in Pima 379.
- Taxon
- cottonGossypium hirsutume1
- Material
- Pima 379e0
- Outcome
- gene was not expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the gene was not expressed in Pima 379”
e1“The cotton chromosome substitution line, CS-B15sh”
Claim · model-written summary
Glutathione S-transferase was differentially expressed in CS-B15sh and Pima 379 but not TM-1.
- Taxon
- cottonGossypium hirsutume1
- Material
- CS-B15she0
- Gene
- glutathione S-transferasee0
- Outcome
- differentially expressede0
- Comparator
- TM-1e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glutathione S-transferase was differentially expressed in both CS-B15sh and Pima 379 but not in TM-1”
e1“The cotton chromosome substitution line, CS-B15sh”
Claim · model-written summary
Cytochrome P450 and redox genes were significantly expressed only in CS-B15sh after 2,4-D.
- Taxon
- cottonGossypium hirsutume1
- Material
- CS-B15she0
- Treatment
- 2,4-De0
- Gene
- cytochrome P450e0
- Outcome
- significantly expressede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cytochrome P450 and other genes involved in the oxidation–reduction process were significantly expressed only in CS-B15sh in response to 2,4-D”
e1“The cotton chromosome substitution line, CS-B15sh”
Claim · model-written summary
Gene-set enrichment found depletion of photosynthesis transcripts.
- Taxon
- cottonGossypium hirsutume1
- Outcome
- transcripts involved in photosynthesise0
- Direction
- depletione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gene set enrichment analysis on the union DEGs of the three cotton genotypes revealed the depletion of transcripts involved in photosynthesis”
e1“The cotton chromosome substitution line, CS-B15sh”
Claim · model-written summary
Gene-set enrichment found enrichment of ABA-response and signaling transcripts.
- Taxon
- cottonGossypium hirsutume1
- Outcome
- transcripts involved in ABA response and signalinge0
- Direction
- enrichmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enrichment of transcripts involved in ABA response and signaling”
e1“The cotton chromosome substitution line, CS-B15sh”
The same questions in other papers
2,4-D · crop injury · Gossypium hirsutum (cotton)Positive and negative results
1 paper in the same direction as this paper · 8 in the opposite direction
- negative
- negative
- no effect
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.