Paper · 2022 · Journal of Experimental Agriculture International

Biocontrol Activity of Yeasts against Alternaria solani and Plant Growth Promotion of Tomato Plants

G. Ashiwini; B. C. Mallesha

10
claims
20
verbatim quotes
2
citations
4
research areas
✓
Open access

Abstract

Aim: Yeasts have emerged as a single celled biocontrol agent which are applicable in controlling many disease causing pathogens in plants. These yeasts also have growth promoting substances that help in the growth and development of plants. Materials and Methods: Yeast on tomato plants were isolated using standard plate count technique and observed under microscope for their cell shape and sizes. Biocontrol activity of yeast was evaluated by measuring diameter of zone of inhibition (mm) and estimating mycelial dry weights of pathogen. IAA production by yeasts was estimated in vitro and the plant growth and development was evaluated by measuring plant heights, number of leaves, number of days for flower initiation, shoot biomass, root mass, disease incidence and severity in pot culture experiments. Results: Yeast isolate TPL-I isolated from tomato plants was most effective in inhibiting Alternaria solani with diameter of zone of inhibition of 9.00 mm. Reduced mycelial dry weight of pathogen viz., 40.07 mg was exhibited by TPL-I under in vitro conditions. The maximum concentration of IAA was produced by TPL- I (0.11 μg/ml). The highest plant height, number of leaflets and number of days taken for flower initiation was recorded highest in T4 (Trichoderma harzianum) followed by T7 (Foliar spray of Alternaria solani + TPL- I + Trichoderma harzianum) at 30, 45 and 60 days after transplanting. Maximum dry weight of shoot (12.32 g/plant) and root (5.18 g/plant) was recorded in T4 (Foliar spray of Trichoderma harzianum) whereas, minimum dry weight of shoot (8.26 g/plant) and root (2.80 g/plant) was recorded in T2 (Foliar spray of Alternaria solani). Lowest disease incidence (33.33%) and disease severity (24.25%) was recorded in T5 (Foliar spray of Alternaria solani + TPL- I).

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Claims 10

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Tomato yeast isolate TPL-I had the strongest in vitro inhibition of Alternaria solani, with a 9.00 mm zone.

Taxon
tomato plantsSolanum lycopersicume0
Material
TPL-Ie0
Interactor
Alternaria solaniAlternaria solanie0pathogen
Outcome
inhibiting Alternaria solani with diameter of zone of inhibitione0
Direction
most effectivee0
Value
9.00 mme0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Alternaria solani
Alternaria solani · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Yeast isolate TPL-I isolated from tomato plants was most effective in inhibiting Alternaria solani with diameter of zone of inhibition of 9.00 mm.”

✓ located verbatim in the source · abstract, characters 818–964 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

TPL-I reduced Alternaria solani mycelial dry weight to 40.07 mg in vitro.

Material
TPL-Ie0
Interactor
Alternaria solaniAlternaria solanie1pathogen
Outcome
mycelial dry weight of pathogene0
Direction
Reducede0
Value
40.07 mge0
Setting
in vitro conditionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Alternaria solani
Alternaria solani · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Reduced mycelial dry weight of pathogen viz., 40.07 mg was exhibited by TPL-I under in vitro conditions.”

✓ located verbatim in the source · abstract, characters 965–1069 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 86–99 · show in text ↑
e1

“Alternaria solani with diameter of zone of inhibition”

✓ located verbatim in the source · abstract, characters 899–952 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

TPL-I produced the highest IAA concentration, 0.11 μg/ml.

Material
TPL- Ie0
Outcome
concentration of IAAe0
Direction
maximume0
Value
0.11 μg/mle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The maximum concentration of IAA was produced by TPL- I (0.11 μg/ml).”

✓ located verbatim in the source · abstract, characters 1070–1139 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 86–99 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Trichoderma harzianum treatment gave the highest plant height, leaflet number and days to flower initiation at 30, 45 and 60 days.

Treatment
T4 (Trichoderma harzianum)e0
Interactor
Trichoderma harzianumTrichoderma harzianume0biocontrol_agent
Outcome
plant height, number of leaflets and number of days taken for flower initiatione0
Direction
higheste0
Qualifier
30, 45 and 60 days after transplantinge0
Comparator
T7 (Foliar spray of Alternaria solani + TPL- I + Trichoderma harzianum)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · InteractorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The highest plant height, number of leaflets and number of days taken for flower initiation was recorded highest in T4 (Trichoderma harzianum) followed by T7 (Foliar spray of Alternaria solani + TPL- I + Trichoderma harzianum) at 30, 45 and 60 days after transplanting.”

✓ located verbatim in the source · abstract, characters 1140–1409 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 86–99 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

T4 Trichoderma spray gave maximum tomato shoot dry weight, 12.32 g/plant.

Treatment
T4 (Foliar spray of Trichoderma harzianum)e0
Interactor
Trichoderma harzianumTrichoderma harzianume0biocontrol_agent
Outcome
dry weight of shoote0
Direction
Maximume0
Value
12.32 g/plante0
Comparator
T2 (Foliar spray of Alternaria solani)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · InteractorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Maximum dry weight of shoot (12.32 g/plant) and root (5.18 g/plant) was recorded in T4 (Foliar spray of Trichoderma harzianum) whereas, minimum dry weight of shoot (8.26 g/plant) and root (2.80 g/plant) was recorded in T2 (Foliar spray of Alternaria solani).”

✓ located verbatim in the source · abstract, characters 1410–1668 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 86–99 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

T4 Trichoderma spray gave maximum tomato root dry weight, 5.18 g/plant.

Treatment
T4 (Foliar spray of Trichoderma harzianum)e0
Interactor
Trichoderma harzianumTrichoderma harzianume0biocontrol_agent
Outcome
dry weight of shoot (12.32 g/plant) and roote0
Direction
Maximume0
Value
5.18 g/plante0
Comparator
T2 (Foliar spray of Alternaria solani)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · InteractorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Maximum dry weight of shoot (12.32 g/plant) and root (5.18 g/plant) was recorded in T4 (Foliar spray of Trichoderma harzianum) whereas, minimum dry weight of shoot (8.26 g/plant) and root (2.80 g/plant) was recorded in T2 (Foliar spray of Alternaria solani).”

✓ located verbatim in the source · abstract, characters 1410–1668 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 86–99 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

T2 Alternaria spray gave minimum tomato shoot dry weight, 8.26 g/plant.

Treatment
T2 (Foliar spray of Alternaria solani)e0
Interactor
Alternaria solaniAlternaria solanie0pathogen
Outcome
dry weight of shoote0
Direction
minimume0
Value
8.26 g/plante0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Alternaria solani
Alternaria solani · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“minimum dry weight of shoot (8.26 g/plant) and root (2.80 g/plant) was recorded in T2 (Foliar spray of Alternaria solani)”

✓ located verbatim in the source · abstract, characters 1546–1667 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 86–99 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

T2 Alternaria spray gave minimum tomato root dry weight, 2.80 g/plant.

Treatment
T2 (Foliar spray of Alternaria solani)e0
Interactor
Alternaria solaniAlternaria solanie0pathogen
Outcome
roote0
Direction
minimume0
Value
2.80 g/plante0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Alternaria solani
Alternaria solani · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“minimum dry weight of shoot (8.26 g/plant) and root (2.80 g/plant) was recorded in T2 (Foliar spray of Alternaria solani)”

✓ located verbatim in the source · abstract, characters 1546–1667 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 86–99 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Combined Alternaria and TPL-I spray gave the lowest tomato disease incidence, 33.33%.

Treatment
T5 (Foliar spray of Alternaria solani + TPL- I)e0
Interactor
Alternaria solaniAlternaria solanie0pathogen
Second interactor
TPL- Ie0biocontrol_agent
Outcome
disease incidencee0
Direction
Loweste0
Value
33.33%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Alternaria solani
Alternaria solani · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Lowest disease incidence (33.33%) and disease severity (24.25%) was recorded in T5 (Foliar spray of Alternaria solani + TPL- I).”

✓ located verbatim in the source · abstract, characters 1669–1797 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 86–99 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Combined Alternaria and TPL-I spray gave the lowest tomato disease severity, 24.25%.

Treatment
T5 (Foliar spray of Alternaria solani + TPL- I)e0
Interactor
Alternaria solaniAlternaria solanie0pathogen
Second interactor
TPL- Ie0biocontrol_agent
Outcome
disease severitye0
Direction
Loweste0
Value
24.25%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Alternaria solani
Alternaria solani · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Lowest disease incidence (33.33%) and disease severity (24.25%) was recorded in T5 (Foliar spray of Alternaria solani + TPL- I).”

✓ located verbatim in the source · abstract, characters 1669–1797 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 86–99 · show in text ↑

Alternaria solani · disease · Solanum lycopersicum (tomato)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
See all 3 papers →

Trichoderma harzianum · growth · Solanum lycopersicum (tomato)Positive and negative results

65 papers: 63 positive, 2 negative or no effect

62 papers in the same direction as this paper · 2 in the opposite direction

  1. positive
  2. no effect
  3. no effect

61 papers more in this group

See all 65 papers →

Alternaria solani · growth · Solanum lycopersicum (tomato)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. negative
See all 2 papers →

application · disease · Solanum lycopersicum (tomato)Positive and negative results

150 papers: 134 positive, 16 negative or no effect

133 papers in the same direction as this paper · 16 in the opposite direction

  1. negative
  2. negative
  3. negative

146 papers more in this group

See all 150 papers →

application · growth · Solanum lycopersicum (tomato)Positive and negative results

253 papers: 225 positive, 28 negative or no effect

224 papers in the same direction as this paper · 26 in the opposite direction · 2 reporting no difference from an alternative

  1. no effect
  2. negative
  3. negative

249 papers more in this group

See all 253 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.