Abstract
SUMMARY Iron deficiency is a major constraint for plant growth in calcareous soils. The interplay between NO 3 − and Fe nutrition affects plant performance under Fe‐deficient conditions. However, how NO 3 − negatively regulates Fe nutrition at the molecular level in plants remains elusive. Here, we showed that the key nitrate transporter NRT1.1 in Arabidopsis plants, especially in the shoots, was markedly downregulated at post‐translational levels by Fe deficiency. However, loss of NRT1.1 function alleviated Fe deficiency chlorosis, suggesting that downregulation of NRT1.1 by Fe deficiency favors plant tolerance to Fe deficiency. Further analysis showed that although disruption of NRT1.1 did not alter Fe levels in both the shoots and roots, it improved the reutilization of apoplastic Fe in shoots but not in roots. In addition, disruption of NRT1.1 prevented Fe deficiency‐induced apoplastic alkalization in shoots by inhibiting apoplastic H + depletion via NO 3 − uptake. In vitro analysis showed that reduced pH facilitates release of cell wall‐bound Fe. Thus, foliar spray with an acidic buffer promoted the reutilization of Fe in the leaf apoplast to enhance plant tolerance to Fe deficiency, while the opposite was true for the foliar spray with a neutral buffer. Thus, downregulation of the shoot‐part function of NRT1.1 prevents apoplastic alkalization to ensure the reutilization of apoplastic Fe under Fe‐deficient conditions. Our findings may provide a basis for elucidating the link between N and Fe nutrition in plants and insight to scrutinize the relevance of shoot‐expressed NRT1.1 to the plant response to stress.
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Claims 9
Claim · model-written summary
Iron deficiency markedly downregulated NRT1.1 post-translationally, especially in shoots.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- shootse0
- Treatment
- Fe deficiencye0
- Gene
- NRT1.1e0
- Outcome
- NRT1.1e0
- Direction
- downregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the key nitrate transporter NRT1.1 in Arabidopsis plants, especially in the shoots, was markedly downregulated at post‐translational levels by Fe deficiency”
Claim · model-written summary
Loss of NRT1.1 function alleviated iron deficiency chlorosis.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- loss of NRT1.1 functione0
- Gene
- NRT1.1e0
- Outcome
- Fe deficiency chlorosise0
- Direction
- alleviatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“loss of NRT1.1 function alleviated Fe deficiency chlorosis”
e1“NRT1.1 in Arabidopsis plants”
Claim · model-written summary
Disruption of NRT1.1 did not alter iron levels in shoots and roots.
- Taxon
- ArabidopsisArabidopsise1
- Organ / stage
- shoots and rootse0
- Treatment
- disruption of NRT1.1e0
- Outcome
- Fe levelse0
- Direction
- did not altere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“disruption of NRT1.1 did not alter Fe levels in both the shoots and roots”
e1“NRT1.1 in Arabidopsis plants”
Claim · model-written summary
Disruption of NRT1.1 improved reutilization of apoplastic iron in shoots.
- Taxon
- ArabidopsisArabidopsise1
- Outcome
- reutilization of apoplastic Fe in shootse0
- Direction
- improvede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it improved the reutilization of apoplastic Fe in shoots but not in roots”
e1“NRT1.1 in Arabidopsis plants”
Claim · model-written summary
Disruption of NRT1.1 did not improve apoplastic iron reutilization in roots.
- Taxon
- ArabidopsisArabidopsise1
- Organ / stage
- rootse0
- Outcome
- reutilization of apoplastic Fee0
- Direction
- not in rootse0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it improved the reutilization of apoplastic Fe in shoots but not in roots”
e1“NRT1.1 in Arabidopsis plants”
Claim · model-written summary
Disruption of NRT1.1 prevented shoot apoplastic alkalization induced by iron deficiency.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- disruption of NRT1.1e0
- Gene
- NRT1.1e0
- Outcome
- apoplastic alkalization in shootse0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“disruption of NRT1.1 prevented Fe deficiency‐induced apoplastic alkalization in shoots by inhibiting apoplastic H + depletion via NO 3 − uptake”
e1“NRT1.1 in Arabidopsis plants”
Claim · model-written summary
Reduced pH facilitated release of cell wall bound iron in vitro.
- Taxon
- ArabidopsisArabidopsise1
- Outcome
- release of cell wall‐bound Fee0
- Direction
- facilitatese0
- Qualifier
- reduced pHe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduced pH facilitates release of cell wall‐bound Fe”
e1“NRT1.1 in Arabidopsis plants”
Claim · model-written summary
Acidic buffer spray promoted leaf apoplastic iron reuse and iron deficiency tolerance.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- foliar spray with an acidic buffere0
- Outcome
- reutilization of Fe in the leaf apoplaste0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“foliar spray with an acidic buffer promoted the reutilization of Fe in the leaf apoplast to enhance plant tolerance to Fe deficiency”
e1“NRT1.1 in Arabidopsis plants”
Claim · model-written summary
Neutral buffer spray had the opposite effect to acidic buffer spray.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- foliar spray with a neutral buffere0
- Outcome
- oppositee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while the opposite was true for the foliar spray with a neutral buffer”
e1“NRT1.1 in Arabidopsis plants”
The same questions in other papers
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- negative
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loss of function · nutrient: content · ArabidopsisPositive and negative results
40 papers in the same direction as this paper · 4 in the opposite direction
- negative
- positive
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41 papers more in this group
See all 45 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.