Abstract
Cadmium (Cd) is a heavy metal nonessential for plants; this toxic metal accumulation in crops has significant adverse effects on human health. The crosstalk between copper (Cu) and Cd has been reported; however, the molecular mechanisms remain unknown. The present study investigated the function of wheat Cu transporter 3D (TaCOPT3D) in Cd tolerance. The TaCOPT3D transcripts significantly accumulated in wheat roots under Cd stress. Furthermore, TaCOPT3D-overexpressing lines were compared to wildtype (WT) plants to test the role of TaCOPT3D in Cd stress response. Under 20 mM Cd treatment, TaCOPT3D-overexpressing lines exhibited more biomass and lower root, shoot, and grain Cd accumulation than the WT plants. In addition, overexpression of TaCOPT3D decreased the reactive oxygen species (ROS) levels and increased the active antioxidant enzymes under Cd conditions. Moreover, the transcription factor (TF) TaWRKY22, which targeted the TaCOPT3D promoter, was identified in the regulatory pathway of TaCOPT3D under Cd stress. Taken together, these results show that TaCOPT3D plays an important role in regulating plant adaptation to cadmium stress through bound by TaWRKY22. These findings suggest that TaCOPT3D is a potential candidate for decreasing Cd accumulation in wheat through genetic engineering.
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Claims 7
Claim · model-written summary
TaCOPT3D transcripts accumulated significantly in wheat roots under Cd stress.
- Taxon
- wheat
Triticum aestivume0 - Organ / stage
- rootse0
- Treatment
- Cd stresse0
- Gene
- TaCOPT3De0
- Outcome
- TaCOPT3D transcriptse0
- Direction
- accumulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The TaCOPT3D transcripts significantly accumulated in wheat roots under Cd stress.”
Claim · model-written summary
TaCOPT3D overexpression yielded more wheat biomass than wild type under 20 mM Cd.
Verbatim
e0“Under 20 mM Cd treatment, TaCOPT3D-overexpressing lines exhibited more biomass and lower root, shoot, and grain Cd accumulation than the WT plants.”
Claim · model-written summary
TaCOPT3D overexpression lowered Cd accumulation in wheat roots, shoots and grain versus wild type under 20 mM Cd.
Verbatim
e0“Under 20 mM Cd treatment, TaCOPT3D-overexpressing lines exhibited more biomass and lower root, shoot, and grain Cd accumulation than the WT plants.”
Claim · model-written summary
TaCOPT3D overexpression decreased reactive oxygen species under Cd conditions.
- Treatment
- overexpression of TaCOPT3De0
- Outcome
- reactive oxygen species (ROS) levelse0
- Direction
- decreasede0
Not reportedTaxonComparator
Verbatim
e0“overexpression of TaCOPT3D decreased the reactive oxygen species (ROS) levels and increased the active antioxidant enzymes under Cd conditions”
Claim · model-written summary
TaCOPT3D overexpression increased active antioxidant enzymes under Cd conditions.
Not reportedTaxonComparator
Verbatim
e0“overexpression of TaCOPT3D decreased the reactive oxygen species (ROS) levels and increased the active antioxidant enzymes under Cd conditions”
Claim · model-written summary
TaWRKY22 targets the TaCOPT3D promoter in the Cd stress regulatory pathway.
Not reportedTaxon
Verbatim
e0“the transcription factor (TF) TaWRKY22, which targeted the TaCOPT3D promoter, was identified in the regulatory pathway of TaCOPT3D under Cd stress”
Claim · model-written summary
The authors propose TaCOPT3D as a genetic engineering candidate to decrease Cd accumulation in wheat.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaCOPT3De0
- Outcome
- decreasing Cd accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaCOPT3D is a potential candidate for decreasing Cd accumulation in wheat through genetic engineering”