Abstract
Six varieties were chosen from three different maturity groups: Galina and NS Princeza (0), Sava and NS Apolo (I), and Rubin and NS Zita (II) in order to examine the effect of priming on germination energy depending on soybean seed age. The seeds were produced in the period from 2012 to 2014, i.e., one- to three-year-old seeds were used. The following treatments were used for priming: distilled water - hydropriming (H2O), ascorbic acid 250 mg/l (ASA), folic acid 15 mg/l (Fol.), hydrogen peroxide 1% (H2O2), and control - non-treated seeds (C). Results showed that the effects of priming depended on the variety, seed age, as well as on the applied treatment. Some varieties reacted to priming very well, while others had an inhibited reaction, so germination energy was significantly reduced. Rubin variety had the most favourable reaction, whereby all treatments of this variety led to an increase of quality, with an average increase of 2-8%. The application of primers on one-year-old soybean seeds had a weaker effect than on two- and three -year-old seeds. However, one-year-old seeds had minor oscillations in quality due to priming. The application of priming on one-year-old seeds can lead to an increase or decrease of germination energy by 3%, while two- and three-year-old seeds reached 7%. The results showed that seed priming in ascorbic acid had a positive effect, while the application of other primers did not affect the value of germination energy, nor did it lead to a decrease of its value. Ascorbic acid activity led to the largest increase in the quality of three-year-old soybean seeds by 2.83%, while the increase in the quality of two- and three-year-old seeds was 1.87%. Therefore, we can conclude that there is no universal use of one single primer, as it might not be suitable for each particular variety and can ultimately result in the seed quality decrease.
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Claims 11
Claim · model-written summary
Priming effects on germination energy depended on variety, seed age, and treatment.
- Taxon
- soybean
Glycine maxt0 - Treatment
- priminge0
- Outcome
- effects of priminge0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“soybean”
e0“the effects of priming depended on the variety, seed age, as well as on the applied treatment”
Claim · model-written summary
Some varieties had significantly reduced germination energy after priming.
- Taxon
- soybean
Glycine maxt0 - Treatment
- priminge0
- Outcome
- germination energye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“soybean”
e0“Some varieties reacted to priming very well, while others had an inhibited reaction, so germination energy was significantly reduced”
Claim · model-written summary
All priming treatments increased Rubin seed quality by 2-8% on average.
- Taxon
- soybean
Glycine maxt0 - Material
- Rubine0
- Treatment
- all treatmentse0
- Outcome
- qualitye0
- Direction
- increasee0
- Value
- 2-8%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“soybean”
e0“Rubin variety had the most favourable reaction, whereby all treatments of this variety led to an increase of quality, with an average increase of 2-8%”
Claim · model-written summary
Priming had a weaker effect on one-year-old than two- and three-year-old seeds.
- Taxon
- soybean
Glycine maxe0 - Organ / stage
- one-year-old soybean seedse0
- Treatment
- primerse0
- Outcome
- effecte0
- Direction
- weakere0
- Comparator
- two- and three -year-old seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“soybean”
e0“The application of primers on one-year-old soybean seeds had a weaker effect than on two- and three -year-old seeds”
Claim · model-written summary
One-year-old seeds had smaller quality oscillations under priming.
- Taxon
- soybean
Glycine maxt0 - Treatment
- priminge0
- Outcome
- oscillations in qualitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“soybean”
e0“one-year-old seeds had minor oscillations in quality due to priming”
Claim · model-written summary
Priming changed one-year-old seed germination energy by up to 3% in either direction.
- Taxon
- soybean
Glycine maxt0 - Treatment
- priminge0
- Outcome
- germination energye0
- Direction
- increase or decreasee0
- Value
- 3%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“soybean”
e0“priming on one-year-old seeds can lead to an increase or decrease of germination energy by 3%”
Claim · model-written summary
Priming changed two- and three-year-old seed germination energy by up to 7%.
- Taxon
- soybean
Glycine maxt0 - Outcome
- seedse0
- Value
- 7%e0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“soybean”
e0“two- and three-year-old seeds reached 7%”
Claim · model-written summary
Ascorbic acid priming positively affected germination energy.
- Taxon
- soybean
Glycine maxt0 - Treatment
- priming in ascorbic acide0
- Outcome
- positive effecte0
- Direction
- positivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“soybean”
e0“seed priming in ascorbic acid had a positive effect”
Claim · model-written summary
Other primers did not affect germination energy.
- Taxon
- soybean
Glycine maxt0 - Treatment
- other primerse0
- Outcome
- germination energye0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“soybean”
e0“the application of other primers did not affect the value of germination energy”
Claim · model-written summary
Ascorbic acid increased three-year-old seed quality by 2.83%.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“soybean”
e0“Ascorbic acid activity led to the largest increase in the quality of three-year-old soybean seeds by 2.83%”
Claim · model-written summary
The authors conclude that no one primer suits every variety and some can reduce seed quality.
- Taxon
- soybean
Glycine maxt0 - Outcome
- no universal use of one single primere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“soybean”
e0“there is no universal use of one single primer, as it might not be suitable for each particular variety and can ultimately result in the seed quality decrease”
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