Abstract
Pears are among the most economically important fruits in the world that are grown in all temperate zones. Pyrus communis L., ‘Pyrodwarf®(S)’ rootstock is one of the gene sources used to improve fruit productivity, rootstock resistance, and tolerance to biotic and abiotic stresses. Traditional propagation of P. communis L. is time-consuming and limited by a short growing season and harsh winter conditions. Therefore, in vitro propagation is a suitable alternative. Murashige and Skoog medium (MS) and woody plant medium (WPM) supplemented with different concentrations of 6-benzyladenine (BA) and kinetin (Kin), individually or in combination, were used for in vitro shoot proliferation. Nodal segments were used as explants. MS medium augmented with indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA) was then used for rooting of microshoots. A combination of 2 mg·L−1 BA and 1 mg·L−1 Kin in MS medium resulted in a significant improvement in shoot proliferation. This combination produced the highest number of shoots (4.352 per explant) and leaves (10.02 per explant). The longest shoots (4.045 cm) were obtained in WPM enriched with 1 mg·L−1 BA. However, these shoots were not suitable for multiplication and rooting steps. The largest number of roots (5.50 per microshoot) was obtained on MS medium augmented with IAA at 1 mg·L−1. The produced plantlets were cultivated in pots filled with perlite and cocopeat (in a ratio of 1:3) and acclimatized gradually in a greenhouse, recording an even 90% survival rate.
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Claims 7
Claim · model-written summary
MS with 2 mg·L−1 BA and 1 mg·L−1 Kin significantly improved shoot proliferation.
- Taxon
- Pyrus communis L.
_Poirier_Fleurs_et_feuilles.jpg?width=60)
Pyrus communise1 - Treatment
- 2 mg·L−1 BA and 1 mg·L−1 Kin in MS mediume0
- Outcome
- shoot proliferatione0
- Direction
- improvemente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
e0“A combination of 2 mg·L−1 BA and 1 mg·L−1 Kin in MS medium resulted in a significant improvement in shoot proliferation”
e1“Pyrus communis L.”
Claim · model-written summary
The BA and Kin combination produced 4.352 shoots per explant, the highest number.
- Taxon
- Pyrus communis L.Pyrus communise2
- Treatment
- 2 mg·L−1 BA and 1 mg·L−1 Kin in MS mediume1
- Outcome
- number of shootse0
- Direction
- higheste0
- Value
- 4.352 per explante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This combination produced the highest number of shoots (4.352 per explant) and leaves (10.02 per explant)”
e1“A combination of 2 mg·L−1 BA and 1 mg·L−1 Kin in MS medium”
e2“Pyrus communis L.”
Claim · model-written summary
The BA and Kin combination produced 10.02 leaves per explant, the highest number.
- Taxon
- Pyrus communis L.Pyrus communise2
- Treatment
- 2 mg·L−1 BA and 1 mg·L−1 Kin in MS mediume1
- Outcome
- leavese0
- Direction
- higheste0
- Value
- 10.02 per explante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This combination produced the highest number of shoots (4.352 per explant) and leaves (10.02 per explant)”
e1“A combination of 2 mg·L−1 BA and 1 mg·L−1 Kin in MS medium”
e2“Pyrus communis L.”
Claim · model-written summary
WPM with 1 mg·L−1 BA produced the longest shoots at 4.045 cm.
- Taxon
- Pyrus communis L.Pyrus communise1
- Treatment
- WPM enriched with 1 mg·L−1 BAe0
- Outcome
- shootse0
- Direction
- longeste0
- Value
- 4.045 cme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The longest shoots (4.045 cm) were obtained in WPM enriched with 1 mg·L−1 BA”
e1“Pyrus communis L.”
Claim · model-written summary
Those longest shoots were unsuitable for multiplication and rooting.
- Taxon
- Pyrus communis L.Pyrus communise1
- Outcome
- not suitable for multiplication and rooting stepse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these shoots were not suitable for multiplication and rooting steps”
e1“Pyrus communis L.”
Claim · model-written summary
MS with 1 mg·L−1 IAA gave the most roots, 5.50 per microshoot.
- Taxon
- Pyrus communis L.Pyrus communise1
- Treatment
- MS medium augmented with IAA at 1 mg·L−1e0
- Outcome
- number of rootse0
- Direction
- largeste0
- Value
- 5.50 per microshoote0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The largest number of roots (5.50 per microshoot) was obtained on MS medium augmented with IAA at 1 mg·L−1”
e1“Pyrus communis L.”
Claim · model-written summary
Acclimatized plantlets had a 90% survival rate in greenhouse pots.
- Taxon
- Pyrus communis L.Pyrus communise1
- Outcome
- survival ratee0
- Value
- 90%e0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The produced plantlets were cultivated in pots filled with perlite and cocopeat (in a ratio of 1:3) and acclimatized gradually in a greenhouse, recording an even 90% survival rate”
e1“Pyrus communis L.”
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