Abstract
Pears are among the most economically important fruits in the world that are grown in all temperate zones. ‘Le-Cont’ rootstock pear is one of the gene sources used to improve fruit productivity, rootstock resistance, and tolerance to biotic and abiotic stresses. Traditional propagation of pear. Pear is time-consuming and limited by a short growing season and hard winter conditions. Therefore, in vitro propagation is a suitable alternative method. Murashige and Skoog medium (MS) and spirulina platensis extract at 5,10 and 20 % supplemented with different concentrations (0.25, 0.5 and 1.0 mg/l) of 6-benzyladenine (BA) and kinetin (Kin), individually or in combination with them. Treatments were used for in vitro shoot proliferation. Nodal segments were used as explants. MS medium augmented was 3mg/l 6-benzyladenine (BA) plus 20 % Spirulina platensis extraction then used for shootlets proliferation of micro-shoots. A combination of 3 mg/l BA and 20 % Spirulina platensis as a growth media resulted in a significant improvement in shoot proliferation. This combination produced the highest number of shoots (2.8 per explant) and leaves (6.3 per explant) similar with those containing MS media plus 20% extraction (2.9 per explant) and leaves (6.8 per explant). The longest shoots (2.97 cm) were obtained in each previous treatment. However, these shoots were similar with those produced from classical multiplication by MS according to ISSR analysis which scored 89.1 % of mono morphism percentage and 10.1 % polymorphism. The ISSR analysis shows the highest similarity index percentage for P5-P9 0.986301.
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Claims 9
Claim · model-written summary
The 3 mg/l BA plus 20 % Spirulina platensis medium significantly improved shoot proliferation.
- Taxon
- pear
_Poirier_Fleurs_et_feuilles.jpg?width=60)
Pyrus communist0 - Treatment
- 3 mg/l BA and 20 % Spirulina platensise0
- Outcome
- shoot proliferatione0
- Direction
- improvemente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
e0“A combination of 3 mg/l BA and 20 % Spirulina platensis as a growth media resulted in a significant improvement in shoot proliferation”
t0“pear”
Claim · model-written summary
The combined medium produced 2.8 shoots per explant.
- Taxon
- pearPyrus communist0
- Treatment
- This combinatione0
- Outcome
- number of shootse0
- Direction
- higheste0
- Value
- 2.8 per explante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This combination produced the highest number of shoots (2.8 per explant) and leaves (6.3 per explant)”
t0“pear”
Claim · model-written summary
The combined medium produced 6.3 leaves per explant.
- Taxon
- pearPyrus communist0
- Treatment
- This combinatione0
- Outcome
- leavese0
- Direction
- higheste0
- Value
- 6.3 per explante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This combination produced the highest number of shoots (2.8 per explant) and leaves (6.3 per explant)”
t0“pear”
Claim · model-written summary
MS medium plus 20% extract produced 2.9 shoots per explant.
- Taxon
- pearPyrus communist0
- Treatment
- MS media plus 20% extractione0
- Outcome
- shootst1
- Value
- 2.9 per explante0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“similar with those containing MS media plus 20% extraction (2.9 per explant) and leaves (6.8 per explant)”
t0“pear”
t1“This combination produced the highest number of shoots (2.8 per explant) and leaves (6.3 per explant)”
Claim · model-written summary
MS medium plus 20% extract produced 6.8 leaves per explant.
- Taxon
- pearPyrus communist0
- Treatment
- MS media plus 20% extractione0
- Outcome
- leavese0
- Value
- 6.8 per explante0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS media plus 20% extraction (2.9 per explant) and leaves (6.8 per explant)”
t0“pear”
Claim · model-written summary
The longest shoots measured 2.97 cm under each previous treatment.
- Taxon
- pearPyrus communist0
- Outcome
- longest shootse0
- Direction
- longeste0
- Value
- 2.97 cme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The longest shoots (2.97 cm) were obtained in each previous treatment”
t0“pear”
Claim · model-written summary
ISSR analysis reported 89.1 % monomorphism in produced shoots relative to classical multiplication.
- Taxon
- pearPyrus communist0
- Outcome
- mono morphism percentagee0
- Value
- 89.1 %e0
- Comparator
- classical multiplication by MSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these shoots were similar with those produced from classical multiplication by MS according to ISSR analysis which scored 89.1 % of mono morphism percentage and 10.1 % polymorphism”
t0“pear”
Claim · model-written summary
ISSR analysis reported 10.1 % polymorphism in produced shoots relative to classical multiplication.
- Taxon
- pearPyrus communist0
- Outcome
- polymorphisme0
- Value
- 10.1 %e0
- Comparator
- classical multiplication by MSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these shoots were similar with those produced from classical multiplication by MS according to ISSR analysis which scored 89.1 % of mono morphism percentage and 10.1 % polymorphism”
t0“pear”
Claim · model-written summary
The highest ISSR similarity index for P5-P9 was 0.986301.
- Taxon
- pearPyrus communist0
- Material
- P5-P9e0
- Outcome
- similarity index percentagee0
- Value
- 0.986301e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ISSR analysis shows the highest similarity index percentage for P5-P9 0.986301”
t0“pear”
The same questions in other papers
N-benzyladenine · growth · Pyrus communis (pear)Results agree
11 papers in the same direction as this paper
- positive
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See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.