Abstract
Abstract This study investigated the antioxidant activity of gum Arabic (GA) during androgenesis in barley anthers. After stress pretreatment the anthers were cultured in the presence of 10 mg L −1 GA (Gm) or on control medium (Cm) and compared with respect to activity of selected antioxidant and respiratory enzymes and endogenous hydrogen peroxide (H 2 O 2 ) content. The anthers from Cm and Gm differed in the strategy against H 2 O 2 overproduction, in that the total peroxidase (POX) activity and the number of POX isoforms were significantly higher in Gm- than in Cm-cultured material. High POX activity on Gm paralleled with H 2 O 2 decrease, suggesting the utilization of this chemical for the POX-mediated cell wall formation and reconstruction during growth of multicellular structures. The total superoxide dismutase (SOD) activity on Cm and Gm were at similar level for most of the culture period but the activity of MnSOD was dozen times higher on Gm and this coincided with high activity of fumarase and cytochrome c oxidase. It indicates close interplay between efficient antioxidative protection and high metabolic rate accompanying efficient androgenesis. Mass spectrometry analysis confirmed the presence of POX and other antioxidative and defense enzymes in protein fraction of GA used in the experiments, however as revealed by 2,2-diphenyl-1-picrylhydrazyl assay, the Gm and Cm displayed similar total antioxidant capacity. Thus, the effect of GA on androgenic cultures of barley can be linked to its influence on the activity of anther antioxidant system, rather than its native antioxidative properties.
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Claims 9
Claim · model-written summary
Gum Arabic medium increased total peroxidase activity and the number of POX isoforms in barley anthers.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Treatment
- Gme1
- Outcome
- total peroxidase (POX) activity and the number of POX isoformse1
- Direction
- significantly highere1
- Comparator
- Cme1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“barley anthers”
e1“the total peroxidase (POX) activity and the number of POX isoforms were significantly higher in Gm- than in Cm-cultured material”
Claim · model-written summary
High POX activity on gum Arabic medium paralleled a decrease in H2O2 in barley anthers.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley anthers”
e2“High POX activity on Gm paralleled with H2O2 decrease, suggesting the utilization of this chemical for the POX-mediated cell wall formation and reconstruction during growth of multicellular structures”
Claim · model-written summary
Total SOD activity was at a similar level on gum Arabic and control media in barley anthers for most of the culture period.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- Gme3
- Outcome
- total superoxide dismutase (SOD) activitye3
- Direction
- similar levele3
- Comparator
- Cme3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley anthers”
e3“The total superoxide dismutase (SOD) activity on Cm and Gm were at similar level for most of the culture period”
Claim · model-written summary
MnSOD activity was dozen times higher on gum Arabic medium in barley anthers.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- Gme4
- Gene
- MnSODe4
- Outcome
- activity of MnSODe4
- Direction
- highere4
- Value
- dozen timese4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley anthers”
e4“the activity of MnSOD was dozen times higher on Gm and this coincided with high activity of fumarase and cytochrome c oxidase”
Claim · model-written summary
High fumarase and cytochrome c oxidase activities coincided with gum Arabic medium in barley anthers.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- Gme4
- Outcome
- activity of fumarase and cytochrome c oxidasee4
- Direction
- increaseinferrede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley anthers”
e4“the activity of MnSOD was dozen times higher on Gm and this coincided with high activity of fumarase and cytochrome c oxidase”
Claim · model-written summary
Close interplay between efficient antioxidative protection and high metabolic rate accompanies efficient androgenesis in barley.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- close interplay between efficient antioxidative protection and high metabolic ratee5
- Qualifier
- accompanying efficient androgenesise5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley anthers”
e5“It indicates close interplay between efficient antioxidative protection and high metabolic rate accompanying efficient androgenesis”
Claim · model-written summary
Mass spectrometry confirmed POX and other antioxidative and defence enzymes in the protein fraction of gum Arabic.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- presence of POX and other antioxidative and defense enzymese6
- Qualifier
- in protein fraction of GAe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley anthers”
e6“Mass spectrometry analysis confirmed the presence of POX and other antioxidative and defense enzymes in protein fraction of GA used in the experiments”
Claim · model-written summary
Gum Arabic medium and control medium displayed similar total antioxidant capacity in barley anthers.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- Gme7
- Outcome
- total antioxidant capacitye7
- Direction
- similare7
- Comparator
- Cme7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley anthers”
e7“as revealed by 2,2-diphenyl-1-picrylhydrazyl assay, the Gm and Cm displayed similar total antioxidant capacity”
Claim · model-written summary
The effect of gum Arabic on barley androgenic cultures is linked to anther antioxidant enzyme activity rather than its native antioxidant properties.
- Taxon
- barleyHordeum vulgaret0
- Compound
- GAe0
- Outcome
- activity of anther antioxidant systeme0
- Qualifier
- rather than its native antioxidative propertiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley anthers”
e0“Thus, the effect of GA on androgenic cultures of barley can be linked to its influence on the activity of anther antioxidant system, rather than its native antioxidative properties”