Abstract
Drought stress severely threatens the yield of cereal crops. Therefore, understanding the molecular mechanism of drought stress response of plants is crucial for developing drought-tolerant cultivars. NAC transcription factors (TFs) play important roles in abiotic stress of plants, but the functions of NAC TFs in sorghum are largely unknown. Here, we characterized a sorghum NAC gene, SbNAC9, and found that SbNAC9 can be highly induced by polyethylene glycol (PEG)-simulated dehydration treatments. We therefore investigated the function of SbNAC9 in drought stress response. Sorghum seedlings overexpressing SbNAC9 showed enhanced drought-stress tolerance with higher chlorophyll content and photochemical efficiency of PSII, stronger root systems, and higher reactive oxygen species (ROS) scavenging capability than wild-type. In contrast, sorghum seedlings with silenced SbNAC9 by virus-induced gene silencing (VIGS) showed weakened drought stress tolerance. Furthermore, SbNAC9 can directly activate a putative peroxidase gene SbC5YQ75 and a putative ABA biosynthesis gene SbNCED3. Silencing SbC5YQ75 and SbNCED3 led to compromised drought tolerance and reduced ABA content of sorghum seedlings, respectively. Therefore, our findings revealed the important role of SbNAC9 in response to drought stress in sorghum and may shed light on genetic improvement of other crop species under drought-stress conditions.
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Claims 10
Claim · model-written summary
PEG-simulated dehydration highly induced SbNAC9.
- Treatment
- polyethylene glycol (PEG)-simulated dehydration treatmentse0
- Gene
- SbNAC9e0
- Outcome
- SbNAC9e0
- Direction
- highly inducede0
SorghumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SbNAC9 can be highly induced by polyethylene glycol (PEG)-simulated dehydration treatments”
t0“Sorghum”
Claim · model-written summary
SbNAC9 overexpression enhanced drought-stress tolerance relative to wild-type seedlings.
- Treatment
- overexpressing SbNAC9e0
- Gene
- SbNAC9e0
- Outcome
- drought-stress tolerancee0
- Direction
- enhancede0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sorghum seedlings overexpressing SbNAC9 showed enhanced drought-stress tolerance with higher chlorophyll content and photochemical efficiency of PSII, stronger root systems, and higher reactive oxygen species (ROS) scavenging capability than wild-type.”
t0“Sorghum”
Claim · model-written summary
SbNAC9 overexpression increased chlorophyll content and PSII photochemical efficiency relative to wild type.
- Treatment
- overexpressing SbNAC9e0
- Gene
- SbNAC9e0
- Outcome
- chlorophyll content and photochemical efficiency of PSIIe0
- Direction
- highere0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sorghum seedlings overexpressing SbNAC9 showed enhanced drought-stress tolerance with higher chlorophyll content and photochemical efficiency of PSII, stronger root systems, and higher reactive oxygen species (ROS) scavenging capability than wild-type.”
t0“Sorghum”
Claim · model-written summary
SbNAC9 overexpression produced stronger root systems than wild type.
- Treatment
- overexpressing SbNAC9e0
- Gene
- SbNAC9e0
- Outcome
- root systemse0
- Direction
- strongere0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sorghum seedlings overexpressing SbNAC9 showed enhanced drought-stress tolerance with higher chlorophyll content and photochemical efficiency of PSII, stronger root systems, and higher reactive oxygen species (ROS) scavenging capability than wild-type.”
t0“Sorghum”
Claim · model-written summary
SbNAC9 overexpression increased ROS scavenging capability relative to wild type.
- Treatment
- overexpressing SbNAC9e0
- Gene
- SbNAC9e0
- Outcome
- reactive oxygen species (ROS) scavenging capabilitye0
- Direction
- highere0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sorghum seedlings overexpressing SbNAC9 showed enhanced drought-stress tolerance with higher chlorophyll content and photochemical efficiency of PSII, stronger root systems, and higher reactive oxygen species (ROS) scavenging capability than wild-type.”
t0“Sorghum”
Claim · model-written summary
Silencing SbNAC9 weakened drought-stress tolerance.
- Treatment
- silenced SbNAC9 by virus-induced gene silencing (VIGS)e0
- Gene
- SbNAC9e0
- Outcome
- drought stress tolerancee0
- Direction
- weakenede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sorghum seedlings with silenced SbNAC9 by virus-induced gene silencing (VIGS) showed weakened drought stress tolerance”
t0“Sorghum”
Claim · model-written summary
SbNAC9 directly activated SbC5YQ75.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SbNAC9 can directly activate a putative peroxidase gene SbC5YQ75 and a putative ABA biosynthesis gene SbNCED3”
t0“Sorghum”
Claim · model-written summary
SbNAC9 directly activated SbNCED3.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SbNAC9 can directly activate a putative peroxidase gene SbC5YQ75 and a putative ABA biosynthesis gene SbNCED3”
t0“Sorghum”
Claim · model-written summary
Silencing SbC5YQ75 compromised drought tolerance.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing SbC5YQ75 and SbNCED3 led to compromised drought tolerance and reduced ABA content of sorghum seedlings, respectively.”
t0“Sorghum”
Claim · model-written summary
Silencing SbNCED3 reduced ABA content.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing SbC5YQ75 and SbNCED3 led to compromised drought tolerance and reduced ABA content of sorghum seedlings, respectively.”
t0“Sorghum”
The same questions in other papers
loss of function · tolerance: abiotic · SorghumPositive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.