Abstract
Cotyledons detached from 4‐d‐old cucumber (Cucumis sativus L.) seedlings were subjected to water stress (air‐drying or PEG‐treatment) to examine the effects of the stress on carbohydrate metabolism. Amylolytic activity in the cotyledon was increased about 6‐fold by water stress within 1 d. The substrate specificity and the action pattern indicated that β‐amylase is responsible for the activity. Activities of azocaseinase, malate dehydrogenase and triose‐phosphate isomerase were not affected by water stress, indicating that the effect of the stress on β‐amylase is rather specific. Cycloheximide‐treatment strongly reduced the enhancement of β‐amylase activity. The hypocotyl of cucumber seedlings also exhibited an increase in the enzyme activity when subjected to water stress. The major free sugars in cucumber cotyledons were glucose, fructose, maltose, and sucrose; sucrose being the most abundant. Sucrose content in excised, unstressed cotyledons increased markedly during the incubation. Changes in other free sugars were small compared with that of sucrose. Starch also accumulated in unstressed cotyledons. In stressed cotyledons more sucrose and less starch accumulated than in unstressed ones. Such results were discussed in relation to the enhancement of β‐amylase activity.
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Claims 11
Claim · model-written summary
Water stress raised cotyledon amylolytic activity about sixfold within one day.
- Taxon
- cucumber (Cucumis sativus L.)
Cucumis sativuse1 - Organ / stage
- cotyledone0
- Treatment
- water stresse0
- Outcome
- Amylolytic activitye0
- Direction
- increasede0
- Value
- about 6‐folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Amylolytic activity in the cotyledon was increased about 6‐fold by water stress within 1 d.”
e1“cucumber (Cucumis sativus L.) seedlings”
Claim · model-written summary
Substrate specificity and action pattern implicated β-amylase in the amylolytic activity.
- Taxon
- cucumber (Cucumis sativus L.)Cucumis sativuse1
- Gene
- β‐amylasee0
- Outcome
- activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The substrate specificity and the action pattern indicated that β‐amylase is responsible for the activity.”
e1“cucumber (Cucumis sativus L.) seedlings”
Claim · model-written summary
Water stress did not affect azocaseinase, malate dehydrogenase, or triose-phosphate isomerase activities.
- Taxon
- cucumber (Cucumis sativus L.)Cucumis sativuse1
- Treatment
- water stresse0
- Outcome
- Activities of azocaseinase, malate dehydrogenase and triose‐phosphate isomerasee0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Activities of azocaseinase, malate dehydrogenase and triose‐phosphate isomerase were not affected by water stress”
e1“cucumber (Cucumis sativus L.) seedlings”
Claim · model-written summary
Cycloheximide strongly reduced water-stress enhancement of β-amylase activity.
- Taxon
- cucumber (Cucumis sativus L.)Cucumis sativuse1
- Treatment
- Cycloheximide‐treatmente0
- Outcome
- enhancement of β‐amylase activitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cycloheximide‐treatment strongly reduced the enhancement of β‐amylase activity.”
e1“cucumber (Cucumis sativus L.) seedlings”
Claim · model-written summary
Water stress increased enzyme activity in cucumber hypocotyls.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- hypocotyle0
- Treatment
- water stresse0
- Outcome
- enzyme activitye0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hypocotyl of cucumber seedlings also exhibited an increase in the enzyme activity when subjected to water stress.”
Claim · model-written summary
Glucose, fructose, maltose and sucrose were the major free sugars, with sucrose most abundant.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- cotyledonse0
- Outcome
- major free sugarse0
- Qualifier
- sucrose being the most abundante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The major free sugars in cucumber cotyledons were glucose, fructose, maltose, and sucrose; sucrose being the most abundant.”
Claim · model-written summary
Sucrose increased markedly in excised unstressed cotyledons during incubation.
- Taxon
- cucumber (Cucumis sativus L.)Cucumis sativuse1
- Organ / stage
- excised, unstressed cotyledonse0
- Outcome
- Sucrose contente0
- Direction
- increased markedlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose content in excised, unstressed cotyledons increased markedly during the incubation.”
e1“cucumber (Cucumis sativus L.) seedlings”
Claim · model-written summary
Other free sugars changed little compared with sucrose.
- Taxon
- cucumber (Cucumis sativus L.)Cucumis sativuse1
- Outcome
- Changes in other free sugarse0
- Direction
- smalle0
- Comparator
- sucrosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Changes in other free sugars were small compared with that of sucrose.”
e1“cucumber (Cucumis sativus L.) seedlings”
Claim · model-written summary
Starch accumulated in unstressed cotyledons.
- Taxon
- cucumber (Cucumis sativus L.)Cucumis sativuse1
- Organ / stage
- unstressed cotyledonse0
- Outcome
- Starche0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Starch also accumulated in unstressed cotyledons.”
e1“cucumber (Cucumis sativus L.) seedlings”
Claim · model-written summary
Stressed cotyledons accumulated more sucrose than unstressed cotyledons.
- Taxon
- cucumber (Cucumis sativus L.)Cucumis sativuse1
- Treatment
- stressed cotyledonse0
- Outcome
- sucrosee0
- Direction
- moree0
- Comparator
- unstressed onese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In stressed cotyledons more sucrose and less starch accumulated than in unstressed ones.”
e1“cucumber (Cucumis sativus L.) seedlings”
Claim · model-written summary
Stressed cotyledons accumulated less starch than unstressed cotyledons.
- Taxon
- cucumber (Cucumis sativus L.)Cucumis sativuse1
- Treatment
- stressed cotyledonse0
- Outcome
- starche0
- Direction
- lesse0
- Comparator
- unstressed onese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In stressed cotyledons more sucrose and less starch accumulated than in unstressed ones.”
e1“cucumber (Cucumis sativus L.) seedlings”
The same questions in other papers
drought · molecular: metabolites · Cucumis sativus (cucumber)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- no effect
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.